TITLE

Development and trends of biosurfactant analysis and purification using rhamnolipids as an example

AUTHOR(S)
Heyd, M.; Kohnert, A.; Tan, T.-H.; Nusser, M.; Kirschhöfer, F.; Brenner-Weiss, G.; Franzreb, M.; Berensmeier, S.
PUB. DATE
July 2008
SOURCE
Analytical & Bioanalytical Chemistry;Jul2008, Vol. 391 Issue 5, p1579
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
During the last few decades, increasing interest in biological surfactants led to an intensification of research for the cost-efficient production of biosurfactants compared with traditional petrochemical surface-active components. The quest for alternative production strains also is associated with new demands on biosurfactant analysis. The present paper gives an overview of existing analytical methods, based on the example of rhamnolipids. The methods reviewed range from simple colorimetric testing to sophisticated chromatographic separation coupled with detection systems like mass spectrometry, by means of which detailed structural information is obtained. High-performance liquid chromatography (HPLC) coupled with mass spectrometry currently presents the most precise method for rhamnolipid identification and quantification. Suitable approaches to accelerate rhamnolipid quantification for better control of biosurfactant production are HPLC analysis directly from culture broth by adding an internal standard or Fourier transform infrared attenuated total reflectance spectroscopy measurements of culture broth as a possible quasi-online quantification method in the future. The search for alternative rhamnolipid-producing strains makes a structure analysis and constant adaptation of the existing quantification methods necessary. Therefore, simple colorimetric tests based on whole rhamnolipid content can be useful for strain and medium screening. Furthermore, rhamnolipid purification from a fermentation broth will be considered depending on the following application.
ACCESSION #
32679636

 

Related Articles

  • Determination of Alkylphenol Ethoxylates in Textiles by Normal-Phase Liquid Chromatography and Reversed-Phase Liquid Chromatography/Electrospray Mass Spectrometry. Chao Wang; Qiang Ma; Xing Wang // Journal of AOAC International;Sep/Oct2007, Vol. 90 Issue 5, p1411 

    The article reports on the development of comprehensive methods based on normal-phase liquid chromatography with ultraviolet detection and reversed-phase liquid chromatography/electrospray mass spectrometry for the determination of alkylphenol ethoxylates (APEOs) in textiles. According to the...

  • Alternative Solvents for Extractables and Leachables Evaluation. Roark, Jennifer M.; Jacques, Mai N.; Tullo, Erica J.; Blakinger, Andrew T.; Lehman, Thomas C. // Pharmaceutical Technology;Aug2013, Vol. 37 Issue 8, p51 

    The article discusses a study on extractables and leachables screening of components of bioprocessing systems and packaging components. It informs that the study used solvents for evaluation of process components such as surfactants that interfere with chromatographic detection to contaminate...

  • Ion-pair hollow-fiber liquid-phase microextraction of the quaternary ammonium surfactant dicocodimethylammonium chloride. Hultgren, Sofie; Larsson, Niklas; Nilsson, Bo F.; Jönsson, Jan Åke // Analytical & Bioanalytical Chemistry;Feb2009, Vol. 393 Issue 3, p929 

    A two-phase hollow-fiber (HF) liquid-phase microextraction (LPME) method was developed for determination of a quaternary ammonium compound surfactant, dicocodimethylammonium chloride, in aqueous samples. The porous HF was fixed on a metal rod support and was impregnated with approximately 6.6...

  • Structural isomers of polyfluorinated di- and tri-alkylated phosphate ester surfactants present in industrial blends and in microwave popcorn bags. Trier, Xenia; Nielsen, Nikoline; Christensen, Jan // Environmental Science & Pollution Research;Sep2011, Vol. 18 Issue 8, p1422 

    Introduction: In this study, we provide strategies for detecting and quantifying the structural isomers of polyfluorinated di- and tri-alkyl surfactants (PFAS) by mass spectrometry (MS). We specifically investigate polyfluorinated dialkylated phosphate ester surfactants ( x:2/ y:2 diPAPS,...

  • CLEAN CHROMATOGRAMS. Thompson, Jonathan E.; Omar, Lena // Science Teacher;Dec2003, Vol. 70 Issue 9, p56 

    Investigates the effectiveness of common laundry agents in removing grass stains. Physical characteristics of common surfactants; Effect of surfactant concentration on the stain removal for the three surfactants tested; Observation of the efficacy of the grass stain removal by various laundry...

  • Recent advances of chromatography and mass spectrometry in lipidomics. Min Li; Zhigui Zhou; Honggang Nie; Yu Bai; Huwei Liu // Analytical & Bioanalytical Chemistry;Jan2011, Vol. 399 Issue 1, p243 

    Lipidomics, as a novel branch of metabolomics, which is aimed at comprehensive analysis of lipids and their biological roles with respect to health and diseases, has attracted increased attention from biological and analytical scientists. As a result of the complexity and diversity of lipids,...

  • Distinct hepatic lipid profile of hypertriglyceridemic mice determined by easy ambient sonic-spray ionization mass spectrometry. Alberici, Luciane; Oliveira, Helena; Catharino, Rodrigo; Vercesi, Anibal; Eberlin, Marcos; Alberici, Rosana // Analytical & Bioanalytical Chemistry;Dec2011, Vol. 401 Issue 5, p1655 

    Easy ambient sonic-spray ionization mass spectrometry (EASI-MS) was used to interrogate the hepatic lipid profiles of hypertriglyceridemic and control normotriglyceridemic mice. The analyses of ex vivo complex lipid mixtures were made directly with EASI-MS without accompanying separation steps....

  • Quantitative and qualitative analyses of human salivary NEFA with gas-chromatography and mass spectrometry. Kulkarni, Bhushan V.; Wood, Karl V.; Mattes, Richard D. // Frontiers in Physiology;Aug2012, Vol. 3, p1 

    Salivary non-esterified fatty acids (NEFA) are proposed to play a role in oral health, oral fat detection, and they may hold diagnostic and prognostic potential. Yet, little is known about the array and concentrations of NEFA in saliva. The aim of the study was to conduct qualitative and...

  • Identifying Compounds Despite Chromatography Limitations: Organophosphates in Treated Sewage. Grange, Andrew H.; Osemwengie, Lantis I.; Sovocool, G. Wayne // LC-GC North America;Nov2003, Vol. 21 Issue 11, p1062 

    Describes a novel high resolution mass spectral technique called ion composition elucidation for identifying compounds in complex mixtures. Limitations of chromatographic analysis; Sample applications in organophosphates in treated sewage.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics