Effect of the immobilisation of DNA aptamers on the detection of thrombin by means of surface plasmon resonance

Ostatná, Veronika; Vaisocherová, Hana; Homola, Jiří; Hianik, Tibor
July 2008
Analytical & Bioanalytical Chemistry;Jul2008, Vol. 391 Issue 5, p1861
Academic Journal
We report a multichannel surface plasmon resonance (SPR) sensor for detection of thrombin via DNA aptamers immobilized on the SPR sensor surface. A detailed investigation of the effect of the immobilisation method on the interaction between thrombin and DNA aptamers is presented. Three basic approaches to the immobilisation of aptamers on the surface of the SPR sensor are examined: (i) immobilisation based on chemisorption of aptamers modified with SH groups, (ii) immobilisation of biotin-tagged aptamers via previously immobilized avidin, neutravidin or streptavidin molecular linkers, and (iii) immobilisation employing dendrimers as a support layer for subsequent immobilisation of aptamers. A level of nonspecific binding of thrombin to immobilized human serum albumin (HSA) for each of the immobilisation methods is determined. Immobilisation of aptamers by means of the streptavidin–biotin system yields the best results both in terms of sensor specificity and sensitivity.


Related Articles

  • Detection of Biomolecular Binding Through Enhancement of Localized Surface Plasmon Resonance (LSPR) by Gold Nanoparticles. Hyung Min Kim; Seung Min Jin; Seok Kee Lee; Min-Gon Kim; Yong-Beom Shin // Sensors (14248220);2009, Vol. 9 Issue 4, p2334 

    To amplify the difference in localized surface plasmon resonance (LSPR) spectra of gold nano-islands due to intermolecular binding events, gold nanoparticles were used. LSPR-based optical biosensors consisting of gold nano-islands were readily made on glass substrates using evaporation and heat...

  • Single-Round Patterned DNA Library Microarray Aptamer Lead Identification. Martin, Jennifer A.; Mirau, Peter A.; Chushak, Yaroslav; Chávez, Jorge L.; Naik, Rajesh R.; Hagen, Joshua A.; Kelley-Loughnane, Nancy // Journal of Analytical Methods in Chemistry;5/14/2015, Vol. 2015, p1 

    A method for identifying an aptamer in a single round was developed using custom DNA microarrays containing computationally derived patterned libraries incorporating no information on the sequences of previously reported thrombin binding aptamers. The DNA library was specifically designed to...

  • Anomalous thermal denaturing of proteins adsorbed to nanoparticles. Teichroeb, J. H.; Forrest, J. A.; Ngai, V.; Jones, L. W. // European Physical Journal E -- Soft Matter;Sep2006, Vol. 21 Issue 1, p19 

    We have used localized surface plasmon resonance (LSPR) to monitor the structural changes that accompany thermal denaturing of bovine serum albumin (BSA) adsorbed onto gold nanospheres of size 5nm-60nm. The effect of the protein on the LSPR was monitored by visible extinction spectroscopy. The...

  • Size-dependent denaturing kinetics of bovine serum albumin adsorbed onto gold nanospheres. Teichroeb, J. H.; Forrest, J. A.; Jones, L. W. // European Physical Journal E -- Soft Matter;Aug2008, Vol. 26 Issue 4, p411 

    We have used localized surface plasmon resonance (LSPR) to monitor the kinetics of thermal denaturing of bovine serum albumin (BSA) adsorbed onto gold nanospheres of size 5 nm-100 nm. The effect of the protein on the LSPR was monitored by visible extinction spectroscopy. The wavelength of the...

  • Graphene oxide-based SPR biosensor chip for immunoassay applications. Chiu, Nan-Fu; Huang, Teng-Yi; Lai, Hsin-Chih; Liu, Kou-Chen // Nanoscale Research Letters;Dec2014, Vol. 9 Issue 1, p1 

    This work develops a highly sensitive immunoassay sensor for use in graphene oxide sheet (GOS)-based surface plasmon resonance (SPR) chips. This sensing film, which is formed by chemically modifying a GOS surface, has covalent bonds that strongly interact with the bovine serum albumin (BSA),...

  • Solution-Phase vs Surface-Phase Aptamer-Protein Affinity from a Label-Free Kinetic Biosensor. Daniel, Camille; Roupioz, Yoann; Gasparutto, Didier; Livache, Thierry; Buhot, Arnaud // PLoS ONE;Sep2013, Vol. 8 Issue 9, p1 

    Aptamers are selected DNA ligands that target biomolecules such as proteins. In recent years, they are showing an increasing interest as potential therapeutic agents or recognition elements in biosensor applications. In both cases, the need for characterizing the mating between the target and...

  • Detection of DNA hybridisation in a diluted serum matrix by surface plasmon resonance and film bulk acoustic resonators. Auer, Sanna; Nirschl, Martin; Schreiter, Matthias; Vikholm-Lundin, Inger // Analytical & Bioanalytical Chemistry;Aug2011, Vol. 400 Issue 5, p1387 

    Nanomolar quantities of single-stranded DNA products ∼100 nucleotides long can be detected in diluted 1% serum by surface plasmon resonance (SPR) and film bulk acoustic resonators (FBARs). We have used a novel FBAR sensor in parallel with SPR and obtained promising results with both the...

  • Characterizing the interaction between aptamers and human IgE by use of surface plasmon resonance. Jinli Wang; Renji Lv; Jingjuan Xu; Danke Xu; Hongyuan Chen // Analytical & Bioanalytical Chemistry;Feb2008, Vol. 390 Issue 4, p1059 

    Human immunoglobulin E (hIgE) is such an important protein, because of its involvement in allergic disease, that it is of significance to study the interactions between it and its recognizing elements. In this report an analytical strategy based on surface plasmon resonance (SPR) was developed...

  • Reversible immobilization of proteins with streptavidin affinity tags on a surface plasmon resonance biosensor chip. Li, Yong-Jin; Bi, Li-Jun; Zhang, Xian-En; Zhou, Ya-Feng; Zhang, Ji-Bin; Chen, Yuan-Yuan; Li, Wei; Zhang, Zhi-Ping // Analytical & Bioanalytical Chemistry;Dec2006, Vol. 386 Issue 5, p1321 

    Dissociation of biotin from streptavidin is very difficult due to their high binding affinity. The re-use of streptavidin-modified surfaces is therefore almost impossible, making devices containing them (e.g. surface plasmon resonance (SPR) sensor chips) expensive. This paper describes a new...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics