TITLE

ADSA-TRIS: a new method to study interfacial phenomena at polymer–aqueous solution interfaces

AUTHOR(S)
Nötzold, Kerstin; Jäger, Stefanie; Michel, Stefan; Reichelt, Senta; Eichhorn, Klaus-Jochen; Gauglitz, Günter; Grundke, Karina
PUB. DATE
July 2008
SOURCE
Analytical & Bioanalytical Chemistry;Jul2008, Vol. 391 Issue 5, p1739
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Axisymmetric drop shape analysis-profile (ADSA-P) was combined with total reflectometric interference spectroscopy (TRIS) in one experimental setup to study the interfacial phenomena at solid–liquid and liquid–vapor interfaces caused by adsorption/desorption (dissolution) of surface-active substances. Using sessile liquid droplets on polymer film/chromium-coated glass substrates that were optically matched with an immersion oil to a TRIS reflection prism, the optical thickness (product of physical thickness d and refractive index n) of the polymer film can be estimated by evaluating the wavelength-dependent intensity of reflected light. The sessile droplet is analyzed simultaneously by an ADSA setup arranged in a transverse direction to the path of the white-light beam of TRIS. From this analysis, the solid–vapor interfacial tension γ lv( t), contact angle θ( t), contact radius r( t), drop volume V( t), and solid–liquid interfacial tension γ sl( t) can be monitored as a function of time. The new method was applied to study polystyrene and poly(4-hydroxystyrene) surfaces in contact with aqueous buffer solutions and with protein solutions. The time-dependent changes in the optical film thickness caused by the adsorption of human serum albumin (HSA) and lysozyme (LSZ) were accompanied by changes in the solid–liquid interfacial tension. From the detailed study of both parameters, conclusions can be drawn with regard to the adsorption kinetics of the proteins on the hydrophobic polystyrene surfaces and to conformational changes occurring within the adsorbed protein layers. [Figure not available: see fulltext.]
ACCESSION #
32679620

 

Related Articles

  • Surface Acoustic Wave (SAW) Resonators for Monitoring Conditioning Film Formation. Hohmann, Siegfried; Kögel, Svea; Brunner, Yvonne; Schmieg, Barbara; Ewald, Christina; Kirschhöfer, Frank; Brenner-Weiß, Gerald; Länge, Kerstin // Sensors (14248220);2015, Vol. 15 Issue 5, p11873 

    We propose surface acoustic wave (SAW) resonators as a complementary tool for conditioning film monitoring. Conditioning films are formed by adsorption of inorganic and organic substances on a substrate the moment this substrate comes into contact with a liquid phase. In the case of implant...

  • Synthesis and Characterization of PEG-Modified Polystyrene Particles and Isothermal Equilibrium Adsorption of Bovine Serum Albumin on these Particles. Chern, Chorng-Shyan; Lee, Cheng-Kang; Liu, Kuan-Chaung // Journal of Polymer Research;Sep2006, Vol. 13 Issue 3, p247 

    A series of monodisperse submicron polystyrene (PS) particles with different surface monomethoxy poly(ethylene glycol) (mPEG) densities were prepared and characterized. The effects of the chemically grafted mPEG chains ( MW = 2,000) on the adsorption of bovine serum albumin (BSA) molecules onto...

  • DETERMINATION OF AEROSOLS POLLUTANTS ON MEMBRANES. 1. MEMBRANES PREPARATION AND CHARACTERIZATION. Aldea, Florian; Craciun, Mihaela Emanuela; Iarca, Ludmila; Nechifor, Gheorghe // Environmental Engineering & Management Journal (EEMJ);Aug2010, Vol. 9 Issue 8, p1097 

    The aim of this work is to produce polysulfone and polyetheretherketone membrane filters with different compositions used in determining pollutants levels (dust and lead aerosol) of gaseous effluents. The studies include composite membranes polysulfone/nanospecies, by comparison with...

  • Local modification of the silicon surface with protein molecules. Paribok, I.; Zhavnerko, G.; Agabekov, V.; Zmachinskaya, Yu.; Yantsevich, A.; Usanov, S. // Russian Journal of General Chemistry;Mar2007, Vol. 77 Issue 3, p363 

    Adsorption of bovine serum albumin, horse radish peroxidase, and green fluorescent protein on the hydrophilic silicon surface was studied. The possibility of preparing microstructured one-, two-, and three-component films on the solid surface by combining the methods of microcontact printing and...

  • Enhanced protein binding on femtosecond laser ablated poly(methyl methacrylate) surfaces. Xiaodong Ma; Haibin Huo; Ming Wei; Lingling Wang; Mengyan Shen; Barry, Carol; Mead, Joey // Applied Physics Letters;4/25/2011, Vol. 98 Issue 17, p171101 

    Poly(methyl methacrylate) (PMMA) substrates were ablated through a fast femtosecond (fs) laser scanning process to create patterns for enhanced protein binding. Typically, two patterns with lines and grids were produced and the protein binding was evaluated by studying the adsorption of...

  • A Novel Affinity Disks for Bovine Serum Albumin Purification. Tuzmen, Nalan; Kalburcu, Tülden; Uygun, Deniz; Akgol, Sinan; Denizli, Adil // Applied Biochemistry & Biotechnology;Jan2015, Vol. 175 Issue 1, p454 

    The adsorption characteristics of bovine serum albumin (BSA) onto the supermacroporous poly(hydroxyethylmethacrylate)-Reactive Green 19 [p(HEMA)-RG] cryogel disks have been investigated in this paper. p(HEMA) cryogel disks were prepared by radical polymerization initiated by N, N, N′,...

  • Thermodynamic parameters of bovine serum albumin adsorption at the surface of various nanoparticles. Mosiagin, I.; Furasova, A.; Kozlova, M.; Osmolowsky, M.; Osmolovskaya, O. // Russian Journal of General Chemistry;Nov2015, Vol. 85 Issue 11, p2654 

    Adsorption of a model protein (bovine serum albumin) at hydroxyapatite and maghemite nanoparticles as examples of promising biomedical materials has been studied. Thermodynamic parameters of the process have been determined, adsorption isotherms for various temperatures have been obtained, and...

  • Adsorption of human serum albumin: Dependence on molecular architecture of the oppositely charged... Sukhishvili, Svetlana A.; Granick, Steve // Journal of Chemical Physics;5/22/1999, Vol. 110 Issue 20, p10153 

    Contrasts the adsorption of human serum albumin onto two solid substrates previously primed with the same polyelectrolyte of net opposite charge to form one of two alternative structures. Formation of the structures either by the adsorption of quaternized poly-4-vinylpyridine (QPVP) or by...

  • Encapsulation of Protein-Polysaccharide HIP Complex in Polymeric Nanoparticles. Gaudana, Ripal; Khurana, Varun; Parenky, Ashwin; Mitra, Ashim K. // Journal of Drug Delivery;2011, p1 

    The objective of the present study is to formulate and characterize a nanoparticulate-based formulation of a macromolecule in a hydrophobic ion pairing (HIP) complex form. So far, HIP complexation approach has been studied only for proteins with molecular weight of 10-20 kDa. Hence, we have...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics