Sensing of oxygen in microtiter plates: a novel tool for screening drugs against pathogenic yeasts

Wesolowski, Janine; Hassan, Rabeay Y. A.; Hodde, Stephan; Bardroff, Christian; Bilitewski, Ursula
July 2008
Analytical & Bioanalytical Chemistry;Jul2008, Vol. 391 Issue 5, p1731
Academic Journal
Most antibiotics were discovered via their inhibition of growth of target organisms. However, yeasts in particular have the capability to adapt metabolic pathways to the availability of nutrients e.g. yeasts can easily switch between respiratory and fermentative pathways in response to oxygen concentration, or can even use both simultaneously. Thus, we cultivated S. cerevisiae BY4741 and C. albicans 1386 in microtiter plates with integrated oxygen sensors to characterize the availability of oxygen for the organisms and to detect influences of fungicides on the oxygen consumption rates. The relevance of the respiratory pathway was indicated by the almost total consumption of oxygen during the first 1–3 h of the cultivation in the microtiter plates, when an increase in turbidity could hardly be seen. Moreover, the sensitivity of S. cerevisiae to inhibitors of the respiratory chain, such as myxothiazol, could be detected via a reduced oxygen consumption rate, whereas no inhibition of growth was observed. Thus, not only was the sensitivity of the test organism for the test compound detectable, but the affected pathway was also highlighted. Other compounds, such as pyrrolnitrin and ambruticin VS-3, inhibited growth of C. albicans 1386 and of S. cerevisiae (only pyrrolnitrin), which was additionally observed as reduced oxygen consumption rates. Thus, the determination of oxygen in microtiter plates via fluorescent dyes is a versatile supplement to standard growth inhibition tests.


Related Articles

  • Azole Fungicides Affect Mammalian Steroidogenesis by Inhibiting Sterol 14α-Demethylase and Aromatase. Zarn, Jürg A.; Brüschweiler, Beat J.; Schlatter, Josef R. // Environmental Health Perspectives;Mar2003, Vol. 111 Issue 3, p255 

    Azole compounds play a key role as antifungals in agriculture and in human mycoses and as nonsteroidal antiestrogens in the treatment of estrogen-responsive breast tumors in postmenopausal women. This broad use of azoles is based on their inhibition of certain pathways of steroidogenesis by...

  • Resistance control is crucial. Spackman, Paul; Abel, Charles // Farmers Weekly;3/11/2005, Vol. 142 Issue 10, p58 

    Presents information on fungicide resistance management strategy. Levels of strobilurin-resistant Septoria; Enhancement of the activity of azoles; Importance of a strategy accounting for various modes of action.

  • Cryptococcus gattii : in vitro susceptibility to the new antifungal albaconazole versus fluconazole and voriconazole. Morera-López, Yolanda; Torres-Rodríguez, Josep M.; Jiménez-Cabello, Teresa; Baró-Tomás, Teresa // Medical Mycology;Sep2005, Vol. 43 Issue 6, p505 

    Minimal inhibitory concentrations (MIC) and minimal fungicidal activity of albaconazole, voriconazole and fluconazole against 55 strains of Cryptococcus gattii , clinically or environmentally isolated in Spain and some Latin American countries, were assessed. By means of the microbroth method...

  • A Study for Testing the Antifungal Susceptibility of Yeast by the Japanese Society for Medical Mycology (JSMM) Method - The Proposal of the Modified JSMM Method 2009. Nishiyama, Yayoi; Abe, Michiko; Ikeda, Reiko; Uno, Jun; Oguri, Toyoko; Shibuya, Kazutoshi; Maesaki, Shigefumi; Mohri, Shinobu; Yamada, Tsuyoshi; Ishibashi, Hiroko; Hasumi, Yayoi; Abe, Shigeru // Japanese Journal of Medical Mycology;Sep2010, Vol. 51 Issue 3, p153 

    The Japanese Society for Medical Mycology (JSMM) method used for testing the antifungal susceptibility of yeast. the MIC end point for azole antifungal agents, is currently set at IC80. It was recently shown, however that there is an inconsistency in the MIC value between the JSMM method and the...

  • Structure-inhibitory activity relationships of pyrrolnitrin analogues on its biosynthesis. Young Soo Keum; Yong-Zhe Zhu; Jeong-Han Kim // Applied Microbiology & Biotechnology;Feb2011, Vol. 89 Issue 3, p781 

    Pyrrolnitrin is a bacterial metabolite, served as a natural lead of agricultural fungicides. In a previous study, fenpiclonil was proven to inhibit the oxidative transformation of aminopyrrolnitrin to pyrrolnitrin, catalyzed by aminopyrrolnitrin oxidase (PrnD). This monooxygenase has an...

  • New azole looks set to beat Opus to top spot. Abel, Charles // Farmers Weekly;6/20/2003, Vol. 138 Issue 24, p58 

    Presents information on prothiocona1zole, an azole fungicide for cereals developed by British firm Bayer. Action on all stem base diseases; Results of the trial on the product conducted by Velcourt; Plan of the company to combine prothioconazole with fluoxastrobin.

  • Chemical oxidative synthesis of polypyrrole particles and functionalization by proteins. Ramanavičius, Arūnas; Mostovojus, Viktoras; Kaušaitė, Asta; Lapėnaitė, Ingrida; Finkelšteinas, Arnonas; Ramanavičienė, Almira // Biologija;2006, Issue 3, p43 

    Polypyrrole particles (Ppy) were chemically synthesized by H2O2 and K2Cr2O7 induced polymerization. We discuss the dependence of several oxidizing agents on the synthesis of polypyrrole particles and covalent immobilization of proteins. During modification of Ppy particles by secondary...

  • Microbial transformations of 2-substituted benzothiazoles. De Wever, H.; Besse, P.; Verachtert, H. // Applied Microbiology & Biotechnology;Dec2001, Vol. 57 Issue 5/6, p620 

    The occurrence of benzothiazoles in the environment seems to be restricted to aquatic compartments and is mainly associated with the manufacture and use of the rubber additive 2-mercaptobenzothiazole (MBT) and its derivatives. Although data on benzothiazole biotransformations in natural...

  • Fine tuning doses in wheat.  // Crops;3/12/2005, p24 

    This article focuses on the main messages to emerge from last season's dose response trials in wheat, and expert advice to help to determine the optimum fungicide doses and combinations this year. Despite widespread high levels of resistance to strobilurins, most products still gave a degree of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics