TITLE

A multianalyte ELISA for immunochemical screening of sulfonamide, fluoroquinolone and ß-lactam antibiotics in milk samples using class-selective bioreceptors

AUTHOR(S)
Adrian, Javier; Pinacho, Daniel G.; Granier, Benoit; Diserens, Jean-Marc; Sánchez-Baeza, Francisco; Marco, M.-Pilar
PUB. DATE
July 2008
SOURCE
Analytical & Bioanalytical Chemistry;Jul2008, Vol. 391 Issue 5, p1703
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A multianalyte ELISA has been developed for the simultaneous determination of the most frequently used antibiotic families in the veterinary field following the typical planar microarray configuration, where the identity of the target analyte is encoded by its location in the detection platform (Master et al. in Drug Discovery Today 11:1007–1011, 2006). To accomplish this aim, two individual enzyme-linked immunosorbent assays for sulfonamide and fluoroquinolone antibiotics and an enzyme-linked receptor assay for ß-lactam antibiotics have been combined. The strategy uses microplates coated with the corresponding haptenized proteins in specific sections of the microplate. The samples are mixed with a cocktail containing the bioreagents, and distributed in the wells of the microplate. Identification of the antibiotic present in a particular sample is consequently accomplished by detecting a positive response on the corresponding microplate section. Since the bioreceptors used show a wide recognition of the congeners of each antibiotic family, the multianalyte method is able to detect more than 25 different antibiotics from the three most important antibiotic families. The detectability reached in full-fat milk samples is below the European maximum residue limits. The accuracy and reliability of this multiplexed bioanalytical method have been demonstrated by analyzing blind spiked samples. [Figure not available: see fulltext.]
ACCESSION #
32679613

 

Related Articles

  • Multiresidue Determination of Quinolone and Fluoroquinolone Antibiotics in Fish and Shrimp by Liquid Chromatography/Tandem Mass Spectrometry. Dufresne, Guy; Fouquet, Andre; Forsyth, Don; Tittlemier, Sheryl A. // Journal of AOAC International;Mar/Apr2007, Vol. 90 Issue 2, p604 

    The article describes a multiresidue method for the measurement of quinolone and fluoroquinolone antibiotics in fish and shrimp by liquid chromatography/tandem mass spectrometry. The method satisfies requirements in analyte specificity, sensitivity and extraction solvent composition to as a...

  • Review on immunoanalytical determination of tetracycline and sulfonamide residues in edible products. Pastor-Navarro, Nuria; Maquieira, Ángel; Puchades, Rosa // Analytical & Bioanalytical Chemistry;Oct2009, Vol. 395 Issue 4, p907 

    The state of the art of immunoanalysis for the determination of tetracycline and sulfonamide residues in food products is reviewed. Special attention is paid to the design and synthesis of haptens, providing an overview of the efforts spent on developing antibiotic screening methods to determine...

  • Ultrasensitive Nano-ELISA for Detecting Sulfadimethoxine in Chicken Tissue. Chi-Fang Peng; Xiao-Hui Duan; Qiu-Li Pan; Li-Qiang Liu; Feng Xue // Journal of Chemistry;2013, p1 

    It is challenging to obtain a highly sensitive enzyme-linked sorbent immunoassay (ELISA) method for the rapid screening of veterinary drug residue in animal tissues. Here we present that a simple and sensitive detection for sulfadimethoxine (SDM) residue in animal tissues was achieved by...

  • Mixed immunoassay design for multiple chemical residues detection. Li, Yanshen; Li, Peng; Luo, Xiangshu; Hao, Zhihui; Wang, Zhanhui; Shen, Jianzhong; Cao, Xingyuan; Zhang, Suxia // Analytical & Bioanalytical Chemistry;Apr2013, Vol. 405 Issue 10, p3307 

    In this research, a mixed immunoassay design for multiple chemical residues detection based on combined reverse competitive enzyme-linked immunosorbent assay (ELISA) procedure was developed. This method integrated two reverse ELISA reactions in one assay by labeling horseradish peroxidase to...

  • Enzyme-linked immunosorbent assay and colloidal gold-based immunochromatographic assay for several (fluoro)quinolones in milk. Sheng, Wei; Li, Yuanzhen; Xu, Xin; Yuan, Meng; Wang, Shuo // Microchimica Acta;Jun2011, Vol. 173 Issue 3/4, p307 

    We have developed a heterologous direct competitive enzyme-linked immunosorbent assay (ELISA) and a visual colloidal gold-based immunochromatographic assay (CGIA) for simultaneous determination of ofloxacin, marbofloxacin, and fleroxacin residues in milk using polyclonal antibodies. The...

  • Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin. Nam-Gun Kim; Myeong-Ae Kim; Young-Il Park; Tae-Sung Jung; Seong-Wan Son; ByungJae So; Hwan-Goo Kang // Journal of Veterinary Science;Dec2015, Vol. 16 Issue 4, p431 

    Monoclonal anti-enrofloxacin antibody was prepared for a direct competitive enzyme-linked immunosorbent assay (ELISA) and purification system using monoclonal antibody (mAb) coupled magnetic nanoparticles (MNPs). The IC50 values of the developed mAb for enrofloxacin (ENR), ciprofloxacin,...

  • Bioactive Dehydrotyrosyl and Dehydrodopyl Compounds of Marine Origin. Sugumaran, Manickam; Robinson, William E. // Marine Drugs;Dec2010, Vol. 8 Issue 12, p2906 

    The amino acid, tyrosine, and its hydroxylated product, 3,4-dihydroxyphenylalanine (dopa), plays an important role in the biogenesis of a number of potentially important bioactive molecules in marine organisms. Interestingly, several of these tyrosyl and dopa-containing compounds possess dehydro...

  • Serogrupos, serosubtipos, inmunotipos y susceptibilidad antimicrobiana de cepas de Neisseria meningitidis aisladas de portadores. Zamora, Leydis; Martínez, Isabel; Gutiérrez, Mercedes; Núñez, Niuris; Ginebra, Mónica; Climent, Yanet; Sotolongo, Franklin; Camaraza, María Amalia // Revista VacciMonitor (Vacunología y Temas Afines);Jan2002, Vol. 11 Issue 1, p1 

    Considering the fundamental role played by asymptomatic Neisseria meningitidis carriers in the transmission of the meningococcal disease, it is necessary to study the issues related to the phenotypical characterization of this microorganism more deeply. With the purpose of contributing to the...

  • Inhibitory effect of rapamycin and dexamethasone on production of IL-17 and IFN-γ in Vogt–Koyanagi–Harada patients. K Yang // British Journal of Ophthalmology;Feb2009, Vol. 93 Issue 2, p249 

    AIMS: To evaluate the effect of rapamycin (RAPA) and dexamethasone (DEX) on the production of IL-17 and IFN-γ by peripheral blood mononuclear cells (PBMCs) from Vogt–Koyanagi–Harada (VKH) patients and healthy individuals. METHODS: Blood samples were drawn from 10 active VKH...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics