TITLE

Two immunoassay formats for fully automated CRP detection in human serum

AUTHOR(S)
Albrecht, Christiane; Kaeppel, Nina; Gauglitz, Guenter
PUB. DATE
July 2008
SOURCE
Analytical & Bioanalytical Chemistry;Jul2008, Vol. 391 Issue 5, p1845
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Immunoassays are a proven approach towards fast, sensitive, cost-effective and easy-to-use analytical systems which are able to measure a variety of interesting analytes, especially in medical diagnostics. Herein, we report two assay formats, binding inhibition and sandwich assay format, for detection of C-reactive protein (CRP) in human serum. Both assays were characterised and compared with respect to their suitability and adaption into a complete sensor system. An automated, optical biosensor system, based on evanescent field technology, was used to carry out a full threefold calibration in each case. Owing to the resulting working ranges, 0.044–2.9 mg L−1 and 0.13–22.9 mg L−1, respectively, the assays qualify for use in detecting high-sensitivity CRP (C-reactive protein).
ACCESSION #
32679599

 

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