Effect of histone deacetylase inhibitor on proliferation of biliary tract cancer cell lines

Li-Ning Xu; Xin Wang; Sheng-Quan Zou; Kozarek, Richard A.
April 2008
World Journal of Gastroenterology;4/28/2008, Vol. 14 Issue 16, p2578
Academic Journal
AIM: To explore the effect of histone deacetylase inhibitor, trichostatin A (TSA) on the growth of biliary tract cancer cell lines (gallbladder carcinoma cell line and cholangiocarcinoma cell line) in vivo and in vitro, and to investigate the perspective of histone deacetylase inhibitor in its clinical application. METHODS: The survival rates of gallbladder carcinoma cell line (Mz-ChA-l cell line) and cholangiocarcinoma cell lines (QBC939, KMBC and OZ cell lines) treated with various doses of TSA were detected by methylthiazoy tetrazolium (MTT) assay. A nude mouse model of transplanted gallbladder carcinoma (Mz-ChA-l cell line) was successfully established, and changes in the growth of transplanted tumor after treated with TSA were measured. RESULTS: TSA could inhibit the proliferation of gallbladder carcinoma cell line (Mz-ChA-l cell line) and cholangiocarcinoma cell lines (QBC939, KMBC and OZ cell lines) in a dose-dependent manner. After the nude mouse model of transplanted gallbladder carcinoma (Mz-ChA-l cell line) was successfully established, the growth of cancer was inhibited in the model after treated with TSA. CONCLUSION: TSA can inhibit the growth of cholangiocarcinoma and gallbladder carcinoma cell lines in vitro and in vivo.


Related Articles

  • CK1ϵ Is Required for Breast Cancers Dependent on β- Catenin Activity. So Young Kim1,4,5,6; Dunn, Ian F.; Firestein, Ron; Gupta, Piyush; Wardwell, Leslie; Repich, Kara; Schinzel, Anna C.; Wittner, Ben; Silver, Serena J.; Root, David E.; Boehm, Jesse S.; Ramaswamy, Sridhar; Lander, Eric S.; Hahn, William C. // PLoS ONE;2010, Vol. 5 Issue 2, p1 

    Background: Aberrant β-catenin signaling plays a key role in several cancer types, notably colon, liver and breast cancer. However approaches to modulate β-catenin activity for therapeutic purposes have proven elusive to date. Methodology: To uncover genetic dependencies in breast cancer...

  • Gallbladder diverticulum: A case report and review of the... Kramer, Andrew J.; Bregman, Andrew; Zeddies, Clark A.; Guynn, Vernon L. // American Surgeon;Apr1998, Vol. 64 Issue 4, p298 

    Discusses the pathophysiology of gallbladder diverticulum. Procedure involved in diagnosing the disease; Two possibilities for the origin of most true diverticula of the gallbladder; Case study of a patient who was diagnosed of the disease.

  • Quantitative cholescintigraphy and bile abnormalities in patients with acalculous biliary pain. Ponce, Julio; Pons, Vicente; Sopena, Ramón; Garrigues, Vicente; Ponce, Marta; Ortiz, Vicente; Pertejo, Virginia // European Journal of Nuclear Medicine & Molecular Imaging;2004, Vol. 31 Issue 8, p1160 

    Acalculous biliary pain has been related to gallbladder dysfunction that produces a gallbladder emptying defect—a condition which favours the development of lithiasis. It is therefore probable that microlithiasis is present in patients with gallbladder dysfunction. The aims of this study...

  • Serum CYFRA 21-1 in Biliary Tract Cancers: A Reliable Biomarker for Gallbladder Carcinoma and Intrahepatic Cholangiocarcinoma. Huang, Li; Chen, Wei; Liang, Peiwen; Hu, Wenjie; Zhang, Kunsong; Shen, Shunli; Chen, Jiancong; Zhang, Zhaohui; Chen, Bin; Han, Yuyan; Meng, Fanyin; DeMorrow, Sharon; Yin, Xiaoyu; Lai, Jiaming; Liang, Lijian // Digestive Diseases & Sciences;May2015, Vol. 60 Issue 5, p1273 

    Background: Biliary tract cancers encompass gallbladder carcinoma, and intrahepatic, perihilar and distal cholangiocarcinoma. Upregulated serum CYFRA 21-1 has been reported in intrahepatic cholangiocarcinoma. Aims: The present study aimed to explore the clinical significance of serum CYFRA 21-1...

  • The role of E-cadherin in the differentiation of gallbladder cancer cells. Mukai, Shinsuke; Miyazaki, Kohji; Yakushiji, Hiroyuki // Cell & Tissue Research;Oct2001, Vol. 306 Issue 1, p117 

    Cell adhesion molecules are essential for development and maintenance of epithelial architecture. To clarify the role of these molecules in the morphology of gallbladder cancers, four human gallbladder cancer cell lines (GB-d1, KMG-C, GBK-1, and G-415) were examined in vitro. They showed...

  • GALLBLADDER DISEASE IN NUNS. Nix, James T. // American Journal of Gastroenterology;Mar1965, Vol. 43 Issue 3, p208 

    Presents information on a study regarding the occurrence of gallbladder diseases among nuns in the U.S. Factors that affect the biliary tract disease in members of Catholic religious orders for women; Features of the standard environment embraced by the religious communities for women; Age...

  • What is the Role of Endoscopy in the Management of Biliary Pancreatitis, and When Should It Be Utilized? Ranson, John H. C.; Brandt, Lawrence; Rogers, Arvey I. // American Journal of Gastroenterology;Jan1990, Vol. 85 Issue 1, p10 

    Deals with the controversy and confusion that surrounds the management of patients with gallstone pancreatitis. Factors contributing to the confusion; Distinction of acute pancreatitis with gallstones from acute biliary disease; Risks associated with endoscopic retrograde...

  • Characteristics of transplanted mouse myeloproliferative disease developed after repeated injections of granulocytic colony-stimulating factor. A. Bigildeev; N. Sats; A. Grishchuk; I. Nifontova; D. Svinareva; N. Drize // Bulletin of Experimental Biology & Medicine;Feb2008, Vol. 145 Issue 2, p270 

    Abstract  Transplanted myeloproliferative disease developed in mice against the background of repeated injections of granulocytic CSF was characterized using morphological and molecular biological methods. It was demonstrated that transplanted myeloproliferative disease had a non-viral...

  • Endosonography/Bile Drainage Combination for Difficult-to-Diagnose Gallbladder Disease. Dill, James E. // Journal of Laparoendoscopic & Advanced Surgical Techniques;Dec98, Vol. 8 Issue 6, p361 

    Focuses on a study which examined the use of combined endoscopic ultrasound and stimulated biliary drainage (EUS/SBD) for diagnosing gallbladder disease. Background on difficulties in diagnosing gallblader disease; Method for performing EUS/SBD; Cost effectiveness of the procedure.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics