TITLE

Gene expression in a starch synthase IIa mutant of barley: changes in the level of gene transcription and grain composition

AUTHOR(S)
Clarke, B.; Liang, R.; Morell, M. K.; Bird, A. R.; Jenkins, C. L. D.; Li, Z.
PUB. DATE
August 2008
SOURCE
Functional & Integrative Genomics;Aug2008, Vol. 8 Issue 3, p211
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The barley shrunken grain mutant M292 has a novel high-amylose starch phenotype caused by a mutation in the starch synthase IIa gene ( SsIIa) located at the starch excess-6 (sex6) locus on chromosome 7H of barley. The loss of SSIIa enzyme activity leads to a decrease in amylopectin synthesis to less than 20% of the levels found in wild-type grains. Detailed composition analysis indicates that the contents of protein, non-starch polysaccharides, lipid, sucrose and hexoses, and fructo-oligosaccharides are increased in mature M292 grain compared to wild type. Using a microarray analysis, we characterize the differences between the transcription profiles of wild-type and mutant barley endosperms at mid-grain fill. The expression changes include genes involved in carbon storage, stress-related genes, and a number of transcripts with unassigned function. The changes in gene expression are discussed in terms of the altered grain composition of the mutant seed.
ACCESSION #
32562680

 

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics