Preimplantation genetic diagnosis as a source of human embryonic stem cell lines

Y., Verlinsky; N., Strelchenko; V., Kukharenko; A., Shkumatov; S., Rechitsky; O., Verlinsky; A., Kuliev
April 2008
Reproductive BioMedicine Online (Reproductive Healthcare Limited;Apr2008 Supplement 3, Vol. 16, pS-15
Academic Journal
Preimplantation genetic diagnosis (PGD) is presently the major source for the establishment of human embryonic stem cell (ESC) lines with genetic abnormalities, providing the basis for studying of the primary mechanisms of genetic disorders, and the development of the approaches for their effective therapy. We have presently developed the genetic disease specific human ESC line repository, consisting of a total of 58 genetically abnormal lines. It contains 10 human ESC lines with chromosomal abnormalities, including two translocations, three trisomies, two triploidies and three sex chromosomal aneuploidies, and 48 with different single gene disorders, 13 obtained from the embryos with autosomal recessive mutations, 12 X-linked and 23 autosomal dominant disorders, the latter including human ESC lines with dynamic mutations and those with genetic predisposition to cancer. The resulting collection represents the world's first human ESC bank with genetic disorders, currently available for stem cell research of genetic and acquired disorders (www.stemride. com).


Related Articles

  • Detection of Multiple Mutations in a Single Cell.  // Fertility Weekly;2/8/2010, p8 

    The article presents a study which determines the effect of preimplantation genetic diagnosis (PGD) in detecting three different mutations in one cell by T.M. Alberola and colleagues in Spain. It outlines how the researchers used PGD and has determined the successful procedure of detecting three...

  • An unhealthy practice.  // Nature;10/20/2005, Vol. 437 Issue 7062, p1065 

    Focuses on the development of techniques that could help make human embryonic stem-cell research morally acceptable. Application of a single-cell biopsy procedure called preimplantation genetic diagnosis (PGD) by Advanced Cell Technology Corp.; Development of the altered nuclear transfer (ANT)...

  • Erythropoietic differentiation of a human embryonic stem cell line harbouring the sickle cell anaemia mutation. Pryzhkova, Marina V.; Peters, Ann; Zambidis, Elias T. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Aug2010, Vol. 21 Issue 2, p196 

    Herein is reported efficient erythropoietic differentiation of a human embryonic stem cell (ESC) line derived from a preimplantation genetic diagnosis (PGD)-screened embryo that harbours the homozygous sickle cell disease (SCD) haemoglobinopathy mutation. This human ESC line possesses typical...

  • Preimplantation genetic diagnosis as a novel source of embryos for stem cell research. Pickering, Sue; Braude, Peter R.; Patel, Minal; Burns, Chris J.; Trussler, Jane; Bolton, Virginia; Minger, Stephen // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Oct2003, Vol. 7 Issue 3, p353 

    The generation of human embryonic stem (hES) cells has captured the public and professional imagination, largely due their potential as a means of overcoming many debilitating and degenerative diseases by cell replacement therapy. Despite this potential, few well-characterized hES cell lines...

  • Addendum.  // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Sep2008 Supplement 2, Vol. 17, pS-31 

    The article presents abstracts of research on breakthroughs in reproductive medicine and technology as of September 2008 including intracytoplasmic morphologically selected sperm injection (IMSI) technique on vitrified/warmed oocytes, preimplantation genetic diagnosis as a source of human...

  • Vitrified blastocysts from Preimplantation Genetic Diagnosis (PGD) as a source for human Embryonic Stem Cell (hESC) derivation. Aran, Begoña; Sole, Miquel; Rodriguez-Pizà, Ignasi; Parriego, Mònica; Muñoz, Yolanda; Boada, Montserrat; Barri, Pere; Izpisúa, Juan; Veiga, Anna // Journal of Assisted Reproduction & Genetics;Oct2012, Vol. 29 Issue 10, p1013 

    Embryos diagnosed as abnormal in Preimplantation Genetic Diagnosis (PGD) cycles are useful for the establishment of human Embryonic Stem Cells (hESC) lines with genetic disorders. These lines can be helpful for drug screening and for the development of new treatments. Vitrification has proved to...

  • Derivation and characterization of the first abnormal human embryonic stem cell carrying a mutated von Hippel Lindau allele. J., De Vos; S., Assou; D., Cerecedo; L., Nadal; T., Anahory; A., Girardet; H., Dechaud; B., Klein; S., Hamamah // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Apr2008 Supplement 3, Vol. 16, pS-34 

    Introduction: Human embryonic stem cells (ESC) have revolutionized the field of stem cells and are the proof of principle of in-vitro pluripotency. Human ESC provide great opportunities for regenerative medicine, pharmacological and toxicological investigation, and for the study of human...

  • Science and ethics of stem cell research. McLaren, Anne // Current Science (00113891);2/25/2007, Vol. 92 Issue 4, p424 

    The article focuses on stem cell research. The author believes that stem cells is significant in the regenerative medicine. She mentions the need to pursue research on adult and embryonic stem (ES) cells. She states that stem cells from cloned embryos can be used in the research on rare genetic...

  • Early embryos can yield stem cells... and survive. Pearson, Helen // Nature;8/24/2006, Vol. 442 Issue 7105, p858 

    The article offers insights into embryonic stem cell research. Embryonic stem cells are extracted from a blastocyst, thereby destroying the embryo. Studies shows that stem-cell lines can be grown from less developed embryos and the process could leave them unscarred. It opens up new possiblities...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics