Electrochemical sensor with flavin-containing monooxygenase for triethylamine solution

Hirokazu Saito; Takeshi Shirai; Hiroyuki Kudo; Kohji Mitsubayashi
June 2008
Analytical & Bioanalytical Chemistry;Jun2008, Vol. 391 Issue 4, p1263
Academic Journal
A bioelectronic sensor for triethylamine (TEA) was developed with a flavin-containing monooxygenase type 3 (FMO-3). The TEA biosensor consisted of a Clark-type dissolved-oxygen electrode and an FMO-3 immobilized membrane. The FMO-3 solution was mixed with a poly(vinyl alcohol) containing stilbazolium groups (PVA-SbQ), coated on to the dialysis membrane, and the membrane was irradiated with a fluorescent light to immobilize the enzyme. In order to amplify the biosensor output, a substrate regeneration cycle, obtained by coupling the monooxygenase with l-ascorbic acid (AsA) as reducing reagent system, was applied. The effect of pH on the determination of TEA was studied. The maximum response was achieved at pH >9.0. A drop of the phosphate buffer solution with the AsA was put on the sensing area of the oxygen electrode, and the FMO-3 immobilized membrane was placed on the oxygen electrode and covered with a supporting Nylon mesh net which was secured with a silicone O-ring. A measurement system for TEA solution was constructed using the FMO-3 biosensor, a personal computer, a computer-controlled potentiostat, and an A/D converter. The FMO-3 biosensor was used to measure TEA solution from 0.5 to 4.0 mmol L−1 with 10.0 mmol L−1 AsA. The biosensor also had good reproducibility, for example a 6.31% coefficient of variation for five measurements, and the output current was maintained over a few hours. In order to improve the selectivity of the TEA biosensor, three type of biosensor with FMO isomer types 1, 3, and 5 were constructed and used to measure nitrogen and sulfur compounds. The outputs of the isomer biosensors indicated individual patterns for each sample solution. The selectivity of TEA biosensor would be improved, and determination of sulfur and nitrogen compounds would be possible, by using the different output of biosensors prepared from different FMO isomers.


Related Articles

  • A low perfusion rate microreactor for continuous monitoring of enzyme characteristics: application to glucose oxidase. Posthuma-Trumpie, G. A.; Venema, K.; Van Berkel, W. J. H.; Korf, J. // Analytical & Bioanalytical Chemistry;Nov2007, Vol. 389 Issue 6, p2029 

    This report describes a versatile and robust microreactor for bioactive proteins physically immobilized on a polyether sulfone filter. The potential of the reactor is illustrated with glucose oxidase immobilized on a filter with a cut-off value of 30 kDa. A flow-injection system was used to...

  • Improvement of the electrochemical determination of antioxidant using cationic micellar environment. Mello, Lucilene Dornelles; dos Reis, Alaécio Pinheiro; Kubota, Lauro Tatsuo // Acta Scientiarum: Technology;Oct-Dec2010, Vol. 32 Issue 4, p421 

    Ascorbic acid (AA) is an important compound of the human diet, in the metabolism acting mainly as exogenous antioxidant. A method for electrochemical detection of Ascorbic acid (AA) with a GCE was developed using a cationic surfactant cetylpyridinium chloride (CPC). The presence of CPC...

  • A dopamine sensor based on a carbon paste electrode modified with DNA-doped poly(3,4-ethylenedioxythiophene). Xu, Guiyun; Wang, Wenting; Li, Beibei; Luo, Zhonglin; Luo, Xiliang // Microchimica Acta;Feb2015, Vol. 182 Issue 3/4, p679 

    An electrochemical dopamine sensor was developed by electrochemical modification of a carbon paste electrode with a biocomposite made from poly(3,4-ethylenedioxythiophene) (PEDOT) that was doped with double stranded or single stranded DNA. The resulting conducting biocomposite displays excellent...

  • A Review on Electrochemical Biosensors: Principles and Applications. Mohammed Asef Iqbal; S. G. Gupta; S. S. Hussaini // Advances in Bioresearch;Dec2012, Vol. 3 Issue 4, p158 

    Electrochemical sensors for multiple analytes were the first scitifically proposed as well as commersialised biosensors. Electrochemical biosensors have been studied for a long time. Presently, typical platform of transducers based on semiconductors and screen printed electrodes is in practice...

  • Hydrogen peroxide biosensor based on the direct electrochemistry of myoglobin immobilized on ceria nanoparticles coated with multiwalled carbon nanotubesby a hydrothermal synthetic method. Jian-Ding Qiu; San-Guan Cui; Ru-Ping Liang // Microchimica Acta;Dec2010, Vol. 171 Issue 3/4, p333 

    novel myoglobin-based electrochemical biosensor was developed. It is based on a nanocomposite prepared from multiwalled carbon nanotubes that were coated with ceria nanoparticles. UV-vis and electrochemical measurements displayed that the nanocomposite provides a biocompatible matrix for the...

  • Flexible micro(bio)sensors for quantitative analysis of bioanalytes in a nanovolume of human lachrymal liquid. Andoralov, Viktor; Shleev, Sergey; Arnebrant, Thomas; Ruzgas, Tautgirdas // Analytical & Bioanalytical Chemistry;Apr2013, Vol. 405 Issue 11, p3871 

    A flexible electrochemical micro(bio)sensor has been designed for determination of several biological compounds, specifically, ascorbate, dopamine, and glucose, in human lachrymal liquid (tears). The microsensor for simultaneous determination of ascorbate and dopamine concentrations was based on...

  • Electrochemical Sensors and Biosensors. Faridbod, Farnoush; Gupta, Vinod Kumar; Zamani, Hassan Ali // International Journal of Electrochemistry;2011, p1 

    The authors reflect on the applications of electrochemical sensors and biosensors. They state that many analytical instruments used in environment, food, pharmaceutical, or clinical laboratories use chemical sensors or biosensors. The authors suggest that the next generation of sensors or...

  • Development of an Electrochemical Biosensor for the Detection of Aflatoxin M1 in Milk. Paniel, Nathalie; Radoi, Antonio; Marty, Jean-Louis // Sensors (14248220);2010, Vol. 10 Issue 10, p9439 

    We have developed an electrochemical immunosensor for the detection of ultratrace amounts of aflatoxin M1 (AFM1) in food products. The sensor was based on a competitive immunoassay using horseradish peroxidase (HRP) as a tag. Magnetic nanoparticles coated with antibody (anti-AFM1) were used to...

  • Organic electrochemical transistors operating with electrolytes of increasing complexity for (Bio)sensing. Tarabella, Giuseppe; Coppedè, Nicola; Mosca, Roberto; Cicoira, Fabio; Iannotta, Salvatore // AIP Conference Proceedings;Sep2012, Vol. 1479 Issue 1, p1880 

    Organic Electrochemical transistors are of great interest for their applications as biosensor devices. Within the blossoming activity in this field our contribution here we show promising recent examples of application in sensing and biosensing based on the properties of electrolytes of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics