Synovial mesenchymal stem cells accelerate early remodeling of tendon-bone healing

Young-Jin Ju; Muneta, Takeshi; Yoshimura, Hideya; Koga, Hideyuki; Sekiya, Ichiro
June 2008
Cell & Tissue Research;Jun2008, Vol. 332 Issue 3, p469
Academic Journal
Tendon-bone healing is important for the successful reconstruction of the anterior cruciate ligament by using the hamstring tendon. Mesenchymal stem cells (MSCs) have attracted much interest because of their self-renewing potential and multipotentiality for possible clinical use. We previously reported that MSCs derived from synovium had a higher proliferation and differentiation potential than the other MSCs that we examined. The purpose of this study was to investigate the effect and mechanism of the implantation of the synovial MSCs on tendon-bone healing in rats. Half of the Achilles’ tendon grafts of rats were inserted into a bone tunnel from the tibial plateau to the tibial tuberosity with a suture-post fixation. The bone tunnel was filled with MSCs labeled with fluorescent marker DiI or without MSCs as the control. The tendon-bone interface was analyzed histologically, and collagen fibers were quantified. At 1 week, the tendon-bone interface was filled with abundant DiI-positive cells, and the proportion of collagen fiber area was significantly higher in the MSC group than in the control group. By 2 weeks, the proportion of oblique collagen fibers, which appeared to be Sharpey’s fibers, was significantly higher in the MSC group than in the control group. At 4 weeks, the interface tissue disappeared, and the implanted tendon appeared to attach to the bone directly in both groups. DiI-labeled cells could no longer be observed. Implantation of synovial MSCs into bone tunnel thus accelerated early remodeling of tendon-bone healing, as shown histologically.


Related Articles

  • Collagen synthesis by mesenchymal stem cells and aortic valve interstitial cells in response to mechanical stretch Ku, Ching-Hsin; Johnson, Philip H.; Batten, Puspa; Sarathchandra, Padmini; Chambers, Rachel C.; Taylor, Patricia M.; Yacoub, Magdi H.; Chester, Adrian H. // Cardiovascular Research;Aug2006, Vol. 71 Issue 3, p548 

    Abstract: Objective: The synthesis of appropriate extracellular matrix by cells in tissue engineered heart valve constructs will be important for the maintenance of valve cusp integrity and function. We have examined and compared the capacity of mesenchymal stem cells to synthesise collagen in...

  • collagen. Peters, Michael // BMA A-Z Family Medical Encyclopedia;2004, p184 

    An encyclopedia entry for "collagen" is presented. It refers to a tough, fibrous protein. Collagen is the major structural protein in the body. It forms an important part of tendons, bones, and connective tissue.

  • Efficacy of a mesenchymal stem cell loaded surgical mesh for tendon repair in rats. Schon, Lew C.; Gill, Nicholas; Thorpe, Margaret; Davis, Joel; Nadaud, Joshua; Jooyoung Kim; Molligan, Jeremy; Zijun Zhang // Journal of Translational Medicine;2014, Vol. 12 Issue 1, p1 

    Objectives The purpose of this study was to investigate the efficacy of a composite surgical mesh for delivery of mesenchymal stem cells (MSCs) in tendon repair. Methods The MSC-loaded mesh composed of a piece of conventional surgical mesh and a layer of scaffold, which supported MSC-embedded...

  • Recombinant mouse SPARC promotes parietal endoderm differentiation and cardiomyogenesis in embryoid bodies. Hrabchak, Christopher; Ringuette, Maurice; Woodhouse, Kimberly // Biochemistry & Cell Biology;Dec2008, Vol. 86 Issue 6, p487 

    In the absence of leukemia inhibitory factor, murine embryonic stem cells cultured in vitro spontaneously aggregate to from three-dimensional embryoid bodies that differentiate to produce hematopoietic, endothelial, muscle, and neuronal cell lineages in a manner recapitulating the events of...

  • Laminin-5 and type I collagen promote adhesion and osteogenic differentiation of animal serum-free expanded human mesenchymal stromal cells. Mittag, Falk; Falkenberg, Eva-Maria; Janczyk, Alexandra; Götze, Marco; Felka, Tino; Aicher, Wilhelm K.; Kluba, Torsten // Orthopedic Reviews;2012, Vol. 4 Issue 4, p160 

    Mesenchymal stromal cells (MSC) are differentiation competent cells and may generate, among others, mature osteoblasts or chondrocytes in vitro and in vivo. Laminin-5 and type I collagen are important components of the extracellular matrix. They are involved in a variety of cellular and...

  • In vitro cartilage formation of composites of synovium-derived mesenchymal stem cells with collagen gel. Yokoyama, Akiko; Sekiya, Ichiro; Miyazaki, Kyosuke; Ichinose, Shizuko; Hata, Yuiro; Muneta, Takeshi // Cell & Tissue Research;Nov2005, Vol. 322 Issue 2, p289 

    Graft implantation is one of the more popular procedures for repairing cartilage defects; however, sacrifices of the donor site have been an issue. Mesenchymal stem cells (MSCs) are a fascinating source for regenerative medicine because they can be harvested in a less invasive manner and are...

  • Urinary excretion of twenty peptides forms an early and accurate diagnostic pattern of acute kidney injury. Metzger, Jochen; Kirsch, Torsten; Schiffer, Eric; Ulger, Perihan; Mentes, Ebru; Brand, Korbinian; Weissinger, Eva M.; Haubitz, Marion; Mischak, Harald; Herget-Rosenthal, Stefan // Kidney International;Dec2010, Vol. 78 Issue 12, p1252 

    Early and accurate detection of acute kidney injury (AKI) is needed to prevent the progression to chronic kidney disease and to improve outcome. Here we used capillary electrophoresis-mass spectrometry to identify urinary peptides predictive of AKI in a training set of 87 urine samples...

  • Mesenchymal Stem Cell Responses to Bone-Mimetic Electrospun Matrices Composed of Polycaprolactone, Collagen I and Nanoparticulate Hydroxyapatite. Phipps, Matthew C.; Clem, William C.; Catledge, Shane A.; Yuanyuan Xu; Hennessy, Kristin M.; Thomas, Vinoy; Jablonsky, Michael J.; Chowdhury, Shafiul; Stanishevsky, Andrei V.; Vohra, Yogesh K.; Bellis, Susan L. // PLoS ONE;2011, Vol. 6 Issue 2, p1 

    The performance of biomaterials designed for bone repair depends, in part, on the ability of the material to support the adhesion and survival of mesenchymal stem cells (MSCs). In this study, a nanofibrous bone-mimicking scaffold was electrospun from a mixture of polycaprolactone (PCL), collagen...

  • The ultrastructural changes of tendon axonal profiles of medial rectus muscles according to duration in patients with intermittent exotropia. Kim, S.-H.; Cho, Y. A.; Park, C.-H.; Uhm, C.-S. // Eye;Aug2008, Vol. 22 Issue 8, p1076 

    PurposeThe goal of this study was to investigate the ultrastructural changes of tendon axonal profiles of medial recti in patients with intermittent exotropia at different ages. In addition, we compared the patterns of degeneration with those of secondary exotropia over time.MethodsThirteen...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics