TITLE

Involvement of TWEAK/Fn14 interaction in the synovial inflammation of RA

AUTHOR(S)
S. Kamijo; A. Nakajima; K. Kamata; H. Kurosawa; H. Yagita; K. Okumura
PUB. DATE
April 2008
SOURCE
Rheumatology;Apr2008, Vol. 47 Issue 4, p442
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Objective. TWEAK, TNF-like weak inducer of apoptosis, induces not only apoptosis of some tumour cells, but also proliferation of endothelial cells, and angiogenesis. It is known that TWEAK induces production of cytokines that are involved in the pathogenesis of RA. However, it is not clear how TWEAK takes part in the synovitis of RA. In this study, we investigated the role of TWEAK/fibroblast growth factor-inducible 14 (Fn14) interaction in the synovitis of RA. Methods. TWEAK and Fn14 expression on RA and OA synovial cells (SCs) were analysed by FACS. Synovial fibroblasts (SFs) or freshly isolated SCs were cultured in the presence or absence of recombinant TWEAK (rTWEAK) and anti-TWEAK or anti-Fn14 mAbs. Cell proliferation, cytokine/chemokine production and intercellular adhesion molecule (ICAM-1) expression were measured by WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium monosodium salt], ELISA and FACS, respectively. Results. TWEAK expression was detected on CD45-positive population in RA synovium, whereas Fn14 was detected on both CD45-positive and CD45-negative populations. Cultured RA and OA SFs showed higher proliferation and produced IL-6, IL-8 and MCP-1 in response to rTWEAK. Cell proliferation and cytokine production of freshly isolated SCs from RA patients were suppressed by anti-TWEAK and anti-Fn14 mAbs. ICAM-1 expression on RA, but not OA, SFs was up-regulated by rTWEAK. Conclusions. These data suggest that TWEAK/Fn14 interaction plays a substantial role in the synovitis of RA, by directly inducing the proliferation of SFs, and by up-regulating the production of inflammatory cytokines/chemokines as well as the expression of ICAM-1.
ACCESSION #
31865342

 

Related Articles

  • SYNOVIAL FLUID ANALYSIS  // Davis's Comprehensive Handbook of Laboratory & Diagnostic Tests ;Jan2006, p1198 

    Synovial fluid analysis is performed via arthrocentesis, an invasive procedure involving insertion of a needle into the joint space. Synovial effusions are associated with disorders or injuries involving the joints. The most commonly aspirated joint is the knee, although samples also can be...

  • Correlation between plasma and synovial fluid basic fibroblast growth factor with radiographic severity in primary knee osteoarthritis. Honsawek, Sittisak; Yuktanandana, Pongsak; Tanavalee, Aree; Saetan, Natthaphon; Anomasiri, Wilai; Parkpian, Vinai // International Orthopaedics;May2012, Vol. 36 Issue 5, p981 

    Purpose: The aim of this study was to investigate plasma and synovial fluid basic fibroblast growth factor (bFGF) levels in patients with primary knee osteoarthritis (OA) and to evaluate the correlation between bFGF levels and disease severity. Methods: Thirty-five patients with knee OA and 15...

  • Isolation of Kingella kingae from Synovial Fluids Using Four Commercial Blood Culture Bottles. Høst, B.; Schumacher, H.; Prag, J.; Arpi, M. // European Journal of Clinical Microbiology & Infectious Diseases;Aug2000, Vol. 19 Issue 8, p608 

    According to the literature, Kingella kingae may be an underdiagnosed cause of joint and bone infections in children. The use of the Bactec blood culture system for culture of joint fluids has dramatically improved the isolation of this fastidious bacterium. The aim of this study was to test the...

  • Automated Evaluation of Synovial and Ascitic Fluids With the Advia 2120 Hematology Analyzer. Froom, Paul; Barak, Mira; Diab, Alaa // American Journal of Clinical Pathology;Dec2013, Vol. 140 Issue 6, p828 

    Objectives: The purpose of this study was to determine if the Advia 2120 hematology analyzer (Siemens Healthcare Diagnostics, Deerfield, IL) can accurately quantify both WBCs and the proportion of neutrophils from synovial and ascitic body fluids. Methods: We analyzed 60 samples on the Advia and...

  • synovia.  // Taber's Cyclopedic Medical Dictionary;2005, p2130 

    A definition of the term "synovia" is presented. It refers to synovial fluid.

  • synovial.  // Taber's Cyclopedic Medical Dictionary;2005, p2130 

    A definition of the term "synovial" is presented. It refers to synovia, the lubricating fluid of the joints.

  • synovial fluid.  // Taber's Cyclopedic Medical Dictionary;2005, p2130 

    A definition of the term "synovial fluid" is presented. It refers to clear viscid lubricating fluid of the joint, bursae, and tendon sheaths. It is secreted by the synovial membrane of a joint.

  • synovial fold.  // Taber's Cyclopedic Medical Dictionary;2005, p2130 

    A definition of the term "synovial fold" is presented. It refers to one of the smooth folds of synovial membrane on the inner surface of the joint capsule.

  • synoviparous.  // Taber's Cyclopedic Medical Dictionary;2005, p2130 

    A definition of the term "synoviparous" is presented. It refers to the formation of synovia.

Share

Other Topics