Estrogenic regulation of gene and protein expression within the amygdala of female mice

Jessica Mong; Russell Romeo; Donald Pfaff
December 2007
Endocrine (1355008X);Dec2007, Vol. 32 Issue 3, p271
Academic Journal
Abstract  Estrogens exert important actions on fear and anxiety in both humans and non-humans. Currently, the mechanisms underlying estrogenic modulation of fear are not known. However, evidence suggests that estrogens may exert their influence on fear and anxiety within the amygdala. The purpose of the present study was to examine the genomic effects of estrogens within the amygdala of female mice using high-density oligonucleotide microarrays. We examined the effects of estrogens on gene expression at 2 and 24 h after an acute subcutaneous injection. Data from the microarrays revealed that 2 h following an acute injection of estradiol, 44 genes were significantly up- or downregulated, and at 24 h, 13 transcripts were significantly up- or downregulated. One interesting estrogen-regulated gene, (CaMKIIα), was downregulated ninefold 2 h following an acute estradiol injection but was not altered 24 h after injection. We further examined estrogen regulation of CaMKIIα, as well as CaMKIIβ and CaMKIV within the amygdala using quantitative PCR and western blot analysis. The data indicate that estrogen decreases CaMKIIα and CaMKIV but not CaMKIIβ gene expression within the amygdala. However, CaMKII protein levels were not different, and CaMKIV protein levels increased 2 h post-EB treatment. These results indicate that estrogen regulates CaMK gene expression and protein levels within the amygdala.


Related Articles

  • Oestrogen,endocrine disrupters and their non- genomic functions: a review of current opinions. Pretorius, E.; Bornman, M. S. // South African Journal of Science;Mar/Apr2005, Vol. 101 Issue 3/4, p125 

    Humans as well as animals are exposed to environmental products that mimic oestrogens, and are known as endocrine disrupters (EDCs). This review gives a general background to how these compounds imitate oestrogens, particularly via the receptors, ER-α and ER-β. In addition to invoking the...

  • Drug and Cell Type-Specific Regulation of Genes with Different Classes of Estrogen Receptor β-Selective Agonists. Paruthiyil, Sreenivasan; Cvoro, Aleksandra; Xiaoyue Zhao; Zhijin Wu; Yunxia Sui; Staub, Richard E.; Baggett, Scott; Herber, Candice B.; Griffin, Chandi; Tagliaferri, Mary; Harris, Heather A.; Cohen, Isaac; Bjeldanes, Leonard F.; Speed, Terence P.; Schaufele, Fred; Leitman, Dale C. // PLoS ONE;2009, Vol. 4 Issue 7, p1 

    Estrogens produce biological effects by interacting with two estrogen receptors, ERa and ERβ. Drugs that selectively target ERa or ERβ might be safer for conditions that have been traditionally treated with non-selective estrogens. Several synthetic and natural ERβ-selective compounds...

  • Estrogen receptor (ER) ß1 and ERßcx/ß2 inhibit ERa function differently in breast cancer cell line MCF7. Omoto, Yoko; Eguchi, Hidetaka; Yamamoto-Yamaguchi, Yuri; Hayashi, Shin-ichi // Oncogene;8/7/2003, Vol. 22 Issue 32, p5011 

    Estrogen receptor (ER) a plays an important role in the proliferation and progression of breast cancer. In order to explore the function of wild-type ERß (ERß1) and its variant form, ERßcx/ß2, stable transformants of ERa-positive breast cancer MCF7 cells with ERß1 or...

  • Association of a G2014A Transition in Exon 8 of the Estrogen Receptor-α Gene with Postmenopausal Osteoporosis. Ongphiphadhanakul, B.; Chanprasertyothin, S.; Payattikul, P.; Saetung, S.; Piaseu, N.; Chailurkit, L.; Rajatanavin, R. // Osteoporosis International;Dec2001, Vol. 12 Issue 12, p1015 

    : We report the association of a newly identified synonymous G2014A single nucleotide polymorphism (SNP) which does not alter the amino acid sequence in exon 8 of the estrogen receptor-α (ERα) gene with osteoporosis in Thai postmenopausal women. Subjects consisted of 228 postmenopausal...

  • Stochastic Model of In-Vivo X4 Emergence during HIV Infection: Implications for the CCR5 Inhibitor Maraviroc. Savkovic, Borislav; Symonds, Geoff; Murray, John M. // PLoS ONE;Jul2012, Vol. 7 Issue 7, p1 

    The emergence of X4 tropic viral strains throughout the course of HIV infection is associated with poorer prognostic outcomes and faster progressions to AIDS than for patients in whom R5 viral strains predominate. Here we investigate a stochastic model to account for the emergence of X4 virus...

  • Estrogen Leads to Reversible Hair Cycle Retardation through Inducing Premature Catagen and Maintaining Telogen. Hui-min Hu; Shou-bing Zhang; Xiao-hua Lei; Zhi-li Deng; Wei-xiang Guo; Zhi-fang Qiu; Shuang Liu; Xin-yue Wang; He Zhang; En-kui Duan // PLoS ONE;Jul2012, Vol. 7 Issue 7, p1 

    Estrogen dysregulation causes hair disorder. Clinical observations have demonstrated that estrogen raises the telogen/ anagen ratio and inhibits hair shaft elongation of female scalp hair follicles. In spite of these clinical insights, the properties of estrogen on hair follicles are poorly...

  • Prenatal Bisphenol A Exposure Alters Sex-Specific Estrogen Receptor Expression in the Neonatal Rat Hypothalamus and Amygdala. Cao, Jinyan; Rebuli, Meghan E.; Rogers, James; Todd, Karina L.; Leyrer, Stephanie M.; Ferguson, Sherry A.; Patisaul, Heather B. // Toxicological Sciences;May2013, Vol. 133 Issue 1, p157 

    Bisphenol A (BPA) exposure is ubiquitous, and in laboratory animals, early-life BPA exposure has been shown to alter sex-specific neural organization, neuroendocrine physiology, and behavior. The specific mechanisms underlying these brain-related outcomes, however, remain largely unknown,...

  • Expression of the murine homologue of FMR2 in mouse brain and during development. Chakrabarti, Lisa; Bristulf, Jesper; Foss, Grethe S.; Davies, Kay E. // Human Molecular Genetics;Mar1998, Vol. 7 Issue 3, p441 

    The expression of the FRAXE fragile site on the human X chromosome is associated with the expansion of a CCG repeat at the 5′ end of the FMR2 gene. The repeat expansion results in transcriptional silencing of the gene and this event has been found to be associated with mild mental handicap...

  • Antidepressants do not modulate estrogen receptor α-mediated gene expression. Hermann, B.; Vollmer, I.; Holsboer, F.; Rupprecht, R. // Journal of Neural Transmission;Oct2001, Vol. 108 Issue 10, p1197 

    Summary. The estrogen receptor α has been shown to be activated both in a ligand-dependent and in a ligand independent fashion. We investigated whether antidepressants may directly activate the estrogen receptor α or enhance ligand-dependent or ligand-independent activation of the estrogen...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics