Effects of recombinant H2 relaxin on the expression of matrix metalloproteinases and tissue inhibitor metalloproteinase in cultured early placental extravillous trophoblasts

Nobuyuki Maruo; Koji Nakabayashi; Senn Wakahashi; Ai Yata; Takeshi Maruo
December 2007
Endocrine (1355008X);Dec2007, Vol. 32 Issue 3, p303
Academic Journal
Abstract  Relaxin promotes softening of the uterine cervix and inhibits uterine contractility in rats, mice and pigs. Little information, however, is available about the role of relaxin in humans. In 2002, LGR7 and LGR8 were discovered to be receptors for relaxin and those receptors were identified in the human placenta. Thus, in this study, effects of recombinant H2 (rH2) relaxin on human early placental extravillous trophoblasts (EVTs) were examined. Isolation of EVTs from early placental trophoblasts was performed using the procedures established in our laboratory. After 48-h subculture, the presence of relaxin receptors in cultured EVTs was characterized by RT-PCR and immunoblotting. The cultured EVTs were treated with different doses (0.3–3 ng/ml) of rH2 relaxin for 24 h. The effects of rH2 relaxin on MMP-2, -3, -9 and TIMP-1 mRNAs levels were examined by real-time RT-PCR. RT-PCR and immunoblotting revealed that relaxin receptors are present in early placental EVTs. Treatment with rH2 relaxin increased MMP-2 and -9 mRNAs levels and decreased TIMP-1 mRNA levels in cultured EVTs, whereas rH2 relaxin did not affect MMP-3 mRNA levels. These results suggest that relaxin may promote the invasive potential of early placental EVTs through up-regulating MMP-2, -9 mRNAs and down-regulating TIMP-1 mRNA in EVTs.


Related Articles

  • Conference Abstracts.  // Fertility Weekly;6/3/2002, p18 

    The article presents various conference abstracts related to pregnancy published in the periodical "Fertility Weekly." According to an abstract presented by authors at the Meeting of the German Society of Endocrinology (DGE), held in Gottingen, Germany, in humans and non-human primates, relaxin...

  • Disparate effects of relaxin and TGFβ1: relaxin increases, but TGFβ1 inhibits, the relaxin receptor and the production of IGFBP‐1 in human endometrial stromal/decidual cells. L. Tseng // Human Reproduction;Jul2004, Vol. 19 Issue 7, p1513 

    BACKGROUND: The purpose of this study was to determine the effect of progestin, relaxin (RLX) and transforming growth factor β1 (TGFβ1) on the content of relaxin receptor (LGR7) mRNA. The effect of RLX on insulin‐like growth factor binding protein‐1 (IGFBP‐1) production...

  • Immuno-neutralization of circulating relaxin does not alter the breast cancer-protective action of parity in MNU-treated rats. Steinetz, Bernard G.; Sherwood, O. David; Lasano, Sally; Horton, Lori; Bosland, Maarten C. // Journal of Experimental Therapeutics & Oncology;2004, Vol. 4 Issue 1, p59 

    Early pregnancy and childbirth protects women against future development of breast cancer by an unknown mechanism. Parity likewise reduces mammary cancer incidence in rats exposed to the carcinogen, N-methyl-N-nitrosourea (MNU), providing a model for the human phenomenon. We hypothesized that...

  • The chemically synthesized human relaxin-2 analog, B-R13/17K H2, is an RXFP1 antagonist. Hossain, Mohammed Akhter; Samuel, Chrishan S.; Binder, Claudia; Hewitson, Tim D.; Tregear, Geoffrey W.; Wade, John D.; Bathgate, Ross A. D. // Amino Acids;Jul2010, Vol. 39 Issue 2, p409 

    Relaxin is a pleiotropic hormone which exerts its biological functions through its G-protein coupled receptor, RXFP1. While relaxin is well known for its reproductive and antifibrotic roles, recent studies suggest that it is produced by cancer cells and acts on RXFP1 to induce growth and...

  • Relaxin-1–deficient mice develop an age-related progression of renal fibrosis. Samuel, Chrishan S.; Chongxin Zhao; Bond, Courtney P.; Hewitson, Tim D.; Amento, Edward P.; Summers, Roger J. // Kidney International;Jun2004, Vol. 65 Issue 6, p2054 

    Relaxin-1–deficient mice develop an age-related progression of renal fibrosis. Background. Relaxin (RLX) is a peptide hormone that stimulates the breakdown of collagen in preparation for parturition and when administered to various models of induced fibrosis. However, its significance in...

  • Relaxin Does Not Improve Angiotensin II-Induced Target-Organ Damage. Haase, Nadine; Rugor, Julianna; Przybyl, Lukasz; Qadri, Fatimunnisa; Müller, Dominik N.; Dechend, Ralf // PLoS ONE;Apr2014, Vol. 9 Issue 4, p1 

    Relaxin is a corpus-luteum produced protein hormone with vasodilatatory, anti-fibrotic, and angiogenic properties that are opposite to angiotensin (Ang) II. We investigated whether or not relaxin ameliorates Ang II-induced target-organ damage. We used double transgenic rats harboring both human...

  • Role of the SLIT/ROBO Pathway in Luteolysis.  // Fertility Weekly;1/19/2009, p3 

    The article discusses the role of SLIT/roundabout (ROBO) pathway to the process of luteolysis in women based from a study conducted in Great Britain. It notes that tissue remodeling with increased apoptosis, matrix metalloproteinase (MMP) activity, and the influx of macrophages are involved in...

  • A histoarchitectural study of early human ectopic pregnancy. Zaidi, M. Tariq; Ansari, M. Salahuddin; Kirmani, Farhan; Khan, Aijaz A. // Biomedical Research (0970-938X);Jan-Mar2012, Vol. 23 Issue 1, p51 

    An ectopic pregnancy is one in which the embryo is implanted outside the uterine cavity. The incidence of such pregnancy has markedly increased in the past decade. This may be a reflection of prompt diagnosis due to easy availability of non-invasive diagnostic tools such as ultrasonography....

  • Circulating levels of matrix proteases and their inhibitors in pregnant women with and without a history of recurrent pregnancy loss. Anumba, Dilly O. C.; Gelany, Saad El; Elliott, Sarah L.; Li, Tin C. // Reproductive Biology & Endocrinology;2010, Vol. 8, p62 

    Background: We have recently shown that serum relaxin-2 levels are attenuated in women with a history of recurrent pregnancy loss (RPL). We sought to determine whether a history of RPL is also associated with changes in serum matrix metalloproteases (MMPs) and tissue inhibitors of matrix...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics