Serial changes in expression of functionally clustered genes in progression of liver fibrosis in hepatitis C patients

Takahara, Yoshiyuki; Takahashi, Mitsuo; Qing-Wei Zhang; Wagatsuma, Hirotaka; Mori, Maiko; Tamori, Akihiro; Shiomi, Susumu; Nishiguchi, Shuhei; Rippe, Richard A.
April 2008
World Journal of Gastroenterology;4/7/2008, Vol. 14 Issue 13, p2010
Academic Journal
AIM: To investigate the relationship of changes in expression of marker genes in functional categories or molecular networks comprising one functional category or multiple categories in progression of hepatic fibrosis in hepatitis C (HCV) patients. METHODS: Marker genes were initially identified using DNA microarray data from a rat liver fibrosis model. The expression level of each fibrosis associated marker gene was analyzed using reverse transcription-polymerase chain reaction (RT-PCR) in clinical biopsy specimens from HCV-positive patients (n = 61). Analysis of changes in expression patterns and interactions of marker genes in functional categories was used to assess the biological mechanism of fibrosis. RESULTS: The profile data showed several biological changes associated with progression of hepatic fibrosis. Clustered genes in functional categories showed sequential changes in expression. Several sets of clustered genes, including those related to the extracellular matrix (ECM), inflammation, lipid metabolism, steroid metabolism, and some transcription factors important for hepatic biology showed expression changes in the immediate early phase (F1/F2) of fibrosis. Genes associated with aromatic amino acid (AA) metabolism, sulfur-containing AA metabolism and insulin/Wnt signaling showed expression changes in the middle phase (F2/F3), and some genes related to glucose metabolism showed altered expression in the late phase of fibrosis (F3/F4). Therefore, molecular networks showing serial changes in gene expression are present in liver fibrosis progression in hepatitis C patients. CONCLUSION: Analysis of gene expression profiles from a perspective of functional categories or molecular networks provides an understanding of disease and suggests new diagnostic methods. Selected marker genes have potential utility for biological identification of advanced fibrosis.


Related Articles

  • Rapid and reliable detection of CD44 variants in gastric carcinoma using a nested reverse transcription-polymerase chain reaction. YAN WANG; YUHONG LIU; BAI XIAO // Oncology Letters;Nov2015, Vol. 10 Issue 5, p2962 

    The present study aimed to establish a rapid and reliable method for detecting the expression of cluster of differentiation 44 variant (CD44v) in gastric carcinoma, and to investigate the significance of CD44v in gastric carcinoma. Using a nested reverse transcription-polymerase chain reaction...

  • Low expression of T-cell transcription factor BCL11b predicts inferior survival in adult standard risk T-cell acute lymphoblastic leukemia patients. Bartram, Isabelle; Goekbuget, Nicola; Schlee, Cornelia; Heesch, Sandra; Fransecky, Lars; Schwartz, Stefan; Stuhlmann, Reingard; Schäfer-Eckhart, Kerstin; Starck, Michael; Reichle, Albrecht; Hoelzer, Dieter; Baldus, Claudia D.; Neumann, Martin // Journal of Hematology & Oncology;2014, Vol. 7 Issue 1, p1 

    Background Risk stratification, detection of minimal residual disease (MRD), and implementation of novel therapeutic agents have improved outcome in acute lymphoblastic leukemia (ALL), but survival of adult patients with T-cell acute lymphoblastic leukemia (T-ALL) remains unsatisfactory. Thus,...

  • Effects of Expression of Transcriptional Factor AP-1 FOSL1 Gene on Psoriatic Process. Sobolev, V. V.; Zolotorenko, A. D.; Soboleva, A. G.; Elkin, A. M.; Il'ina, S. A.; Serov, D. N.; Potekaev, N. N.; Tkachenko, S. B.; Minnibaev, M. T.; Piruzyan, A. L. // Bulletin of Experimental Biology & Medicine;Mar2011, Vol. 150 Issue 5, p632 

    We performed quantitative analysis of FOSL1 gene expression in lesional psoriatic skin. The expression of this gene in lesional psoriatic skin was significantly increased compared to that in unaffected areas. Enhanced FOSL1 expression significantly correlated with high psoriasis area and...

  • Expression Analysis of an R3-Type MYB Transcription Factor CPC-LIKE MYB4 (TRICHOMELESS2) and CPL4-Related Transcripts in Arabidopsis. Tominaga-Wada, Rumi; Nukumizu, Yuka // International Journal of Molecular Sciences;Mar2012, Vol. 13 Issue 3, p3478 

    The CAPRICE (CPC)-like MYB gene family encodes R3-type MYB transcription factors in Arabidopsis. There are six additional CPC-like MYB sequences in the Arabidopsis genome, including TRYPTICHON (TRY), ENHANCER OF TRY AND CPC1 and 2 (ETC1 and ETC2), ENHANCER OF TRY AND CPC3/CPC-LIKE MYB3...

  • Comparative analysis of sphingomyelin synthase 1 gene expression at the transcriptional and translational levels in human tissues. Limborska, Svetlana; Dergunova, Lyudmila; Sudarkina, Olga; Filippenkov, Ivan; Brodsky, Ilya // Molecular & Cellular Biochemistry;Aug2015, Vol. 406 Issue 1/2, p91 

    Sphingomyelin synthase 1 (SMS1) catalyses the biosynthesis of sphingomyelin in eukaryotic cells. We have previously determined the structure of the SGMS1 gene encoding this enzyme and a number of its alternative transcripts. Here, we describe a study of the expression of the full-length SMS1...

  • HEPATITIS C, RURAL AREAS HAVE "ICEBERGS" TOO.  // Gut;Apr2004 Supplement 3, Vol. 53, pA82 

    It is only the tip of the hepatitis C virus (HCV) "iceberg" that is seen for assessment; most data come from urban studies. North Cumbria Acute Hospitals NHS Trust (NCAHT) serves a rural community of 320 000; the size of the HCV problem is unknown. NCAHT receives all serology from primary and...

  • Gene expression and hepatitis C virus infection. T Asselah // Gut;Jun2009, Vol. 58 Issue 6, p846 

    Hepatitis C virus (HCV) is a major cause of chronic liver disease, with about 170 million people infected worldwide. Up to 70% of patients will have persistent infection after inoculation, making this disease a significant cause of morbidity and mortality. The severity of disease varies widely,...

  • Hepatitis C infection, reinfection, and immunity.  // Patient Care;1/15/1992, Vol. 27 Issue 1, p212 

    Discusses research being done on the lack of protective immunity against reinfection with hepatitis C virus (HCV). Reference to a study by P. Farci and colleagues, published in a 1992 issue of "Science" journal; Findings of the polymerase chain reaction analysis of the HCV RNA in chimpanzees...

  • Correlation Study Between HCV Genotypes Distribution Pattern and Viral Load in a Tertiary Care Hospital in Kolkata, India. BHATTACHARJEE, DEBOJYOTI; MUKHERJEE, KHEYA; CHAKROBORTI, GOUTAM; GHOSH, RANADEEP; MANDAL, NABARUN; BOSE, MOHUA // Journal of Clinical & Diagnostic Research;May2015, Vol. 9 Issue 5, p15 

    Background: Hepatitis C virus infection is a leading cause for chronic liver disease. It has wide population specific genotype variability. Genotype knowledge and viral load assessment are equally important for designing therapeutic strategies and as predictors of treatment outcome among...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics