TITLE

Detection of Polyfunctional Mycobacterium tuberculosis—Specific T Cells and Association with Viral Load in HIV-1—Infected Persons

AUTHOR(S)
Day, Cheryl L.; Mkhwanazi, Nompumelelo; Reddy, Sharon; Mncube, Zenele; van der Stok, Mary; Klenerman, Paul; Walker, Bruce D.
PUB. DATE
April 2008
SOURCE
Journal of Infectious Diseases;4/1/2008, Vol. 197 Issue 7, p990
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background. The human immunodeficiency virus type 1 (HIV-1) epidemic is associated with a significant increase in the incidence of tuberculosis (TB); however, little is known about the quality of Mycobacterium tuberculosis (MTB)-specific cellular immune responses in coinfected individuals. Methods. A total of 137 HIV-1-positive individuals in Durban, South Africa, were screened with the use of overlapping peptides spanning Ag85A, culture filtrate protein 10 (CFP-10), early secretory antigen target 6 (ESAT-6), and TB10.4, in an interferon (IFN)-γ enzyme-linked immunospot (ELISPOT) assay. Intracellular cytokine staining for MTB-specific production of IFN-γ, tumor necrosis factor (TNF)-α, and interleukin (IL)-2 was performed, as was ex vivo phenotyping of memory markers on MTB-specific T cells. Results. A total of 41% of subjects responded to ESAT-6 and/or CFP-10, indicating the presence of latent MTB infection. The proportion of MTB-specific IFN-γ+/TNF-α+ CD4+ cells was significantly higher than the proportion of IFN-α+/IL-2+ CD4+ cells (P = .0220), and the proportion of MTB-specific IL-2-secreting CD4 cells was inversely correlated with the HIV-1 load (P = .0098). MTB-specific CD8 T cells were predominately IFN-α+/TNF-α+/IL-2-. Ex vivo memory phenotyping of MTB-specific CD4 and CD8 T cells indicated an early to intermediate differentiated phenotype for the population of effector memory cells. Conclusions. Polyfunctional MTB-specific CD4 and CD8 T cell responses are maintained in the peripheral blood of HIV-1-positive individuals, in the absence of active disease, and the functional capacity of these responses is affected by HIV-1 disease status.
ACCESSION #
31779010

 

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