TITLE

Analysis of nitrophenols in cloud water with a miniaturized light-phase rotary perforator and HPLC-MS

AUTHOR(S)
Hofmann, Diana; Hartmann, Franziska; Herrmann, Hartmut
PUB. DATE
May 2008
SOURCE
Analytical & Bioanalytical Chemistry;May2008, Vol. 391 Issue 1, p161
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
An all-glass miniaturized light-phase rotary perforator for the enrichment of polar compounds has been modified/miniaturized and applied. Its application is demonstrated here for the analysis of nitrophenols and dinitrophenols from low-concentration/low-volume samples. For the method development of high-performance liquid chromatography–mass spectrometry (MS) four eluents were tested: (1) water–methanol, (2) acetic acid–methanol, (3) trifluoroacetic acid–methanol and (4) water–acetonitrile. The last eluent mentioned was used for the subsequent investigation of samples from field experiments. Detection limits varied between 1 ng and 50 pg. The relative standard deviation in repeated measurements was below 15%, corresponding to a good reproducibility. Recoveries ranged between 31 and 100%, showing a significant dependence on the extraction time and the final volume of the sample after evaporation. Quantification was carried out by using deuterated 4-nitrophenol and 2,4-dinitrophenol as standards and applying previously determined response factors. Structure determination of further substances under atmospheric pressure chemical ionization was performed by a first screening with a source collision-induced dissociation, followed by the definite analysis by MS n . The first results are shown for cloud water, fog water and rainwater samples from different locations.
ACCESSION #
31722411

 

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