TITLE

IMMUNOPHENOTYPING - A TOOL FOR THE DIFFERENTIAL DIAGNOSTICS OF LYMPHADENOMEGALY

AUTHOR(S)
Pikalova, Zuzana; Szotkowski, Tomas; Indrak, Karel
PUB. DATE
June 2007
SOURCE
Biomedical Papers of the Medical Faculty of Palacky University i;2007, Vol. 151 Issue 1, p99
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Backround: Flow cytometry is a method that enables the automated quantification of a set of parameters for a large number of cells or cell-like particles. It is also possible to analyze solid tissues after reduction to a single cell suspension. One of the applications of flow cytometry is immunophenotyping. Aim: The authors try to introduce the basic physical and biological principles of flow-cytometry to the broader public and to prove the benefits of method in lymph nodes assessment. Method: A common method of immunophenotyping with the help of labeled monoclonal antibodies applied to lymph node cells was used. Results: Flow cytometry revealed the infiltration of the extirpated lymph node with leukemic cells. Conclusions: The authors demonstrate flow cytometry as a relatively less common but efficient way of lymph node assessment in a patient with an anamnesis of acute lymphoblastic leukemia. They also discuss the contribution of immunophenotyping to the process of the differential diagnostics of lymph nodes enlargement.
ACCESSION #
31371737

 

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