TITLE

Determination of IGF-I in horse plasma by LC electrospray ionisation mass spectrometry

AUTHOR(S)
Popot, Marie-Agnes; Woolfitt, Adrian R.; Garcia, Patrice; Tabet, Jean-Claude
PUB. DATE
April 2008
SOURCE
Analytical & Bioanalytical Chemistry;Apr2008, Vol. 390 Issue 7, p1843
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The insulin-like-growth factor (IGF-I) peptide is considered to be the main indirect marker for growth hormone administration (GH) in a horse. Further to a previous investigation on measurement of IGF-I in plasma samples by mass spectrometry, this study focuses on quantitative and qualitative analysis of intact IGF-I in horse plasma. First, protein-transposing software has been developed for IGF-I to facilitate its quantification by HPLC–electrospray–ion-trap mass spectrometry. Second, product-ion scan experiments on IGF-I have been conducted on standard samples, non-fortified equine plasma samples, fortified plasma samples, and equine GH post-administration samples. This “top-down” approach method enables characterisation of fragment ions corresponding to the carboxy terminal end, which can be useful for the confirmation of the presence of IGF-I in plasma samples. [Figure not available: see fulltext.]
ACCESSION #
31342647

 

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