TITLE

Contribution of multi-nuclear solid state NMR to the characterization of the Thalassiosira pseudonana diatom cell wall

AUTHOR(S)
Tesson, Benoit; Masse, Sylvie; Laurent, Guillaume; Maquet, Jocelyne; Livage, Jacques; Martin-Jézéquel, Véronique; Coradin, Thibaud
PUB. DATE
April 2008
SOURCE
Analytical & Bioanalytical Chemistry;Apr2008, Vol. 390 Issue 7, p1889
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A major issue in the study of biosilicification processes is the harsh chemical conditions required for silica dissolution, which often lead to denaturation of the associated bio-organic matter. In order to demonstrate the potential of solid state NMR for investigating silicified materials of natural origin, this technique was applied to isotopically enriched Thalassiosira pseudonana diatom cells. 29Si, 1H,31P, 13C and 15N solid state NMR studies were performed on whole cells, SDS-extracted and H2O2-cleaned silica shells. Cross-polarization techniques were useful for identifying the presence of mobile and rigid molecules, allowing loosely bound and silica-entrapped species to be discriminated. Successive cleaning procedures efficiently eliminated weakly associated organic matter. The H2O2-cleaned silica shell still contained carbohydrates (mainly chitin) and proteins as well as lipids. This suggests that the role of lipids in diatom shell formation may have been underestimated so far, demonstrating the potential of solid state NMR for studying composite biomaterials.
ACCESSION #
31342644

 

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