TITLE

Home-built integrated microarray system (IMAS). A three-laser confocal fluorescence scanner coupled with a microarray printer

AUTHOR(S)
Tragoulias, Sotirios S.; Obeid, Pierre J.; Tataridis, Ioannis E.; Christopoulos, Theodore K.
PUB. DATE
March 2008
SOURCE
Analytical & Bioanalytical Chemistry;Mar2008, Vol. 390 Issue 6, p1563
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Microarray technology covers the urgent need to exploit the accumulated genetic information from large-scale sequencing projects and facilitate investigations on a genome-wide scale. Although most applications focus on DNA microarrays, the technology has expanded to microarrays of proteins, peptides, carbohydrates, and small molecules aiming either at detection/quantification of biomolecules or investigation of biomolecular interactions in a massively parallel manner. Microarray experiments require two specialized instruments: An arrayer (or printer), for construction of microarrays, and a readout instrument (scanner). We have designed, constructed, and characterized the first integrated microarray system (IMAS) that combines the functions of a microarrayer and a three-laser confocal fluorescence scanner into a single instrument and provides excellent flexibility for the researcher. The three-axis robotic system that moves the printing head carrying multiple pins for arraying is also used for moving the microarray slide in front of a stationary optical system during scanning. Since the translation stages are the most expensive and crucial components of microarray printers and scanners, the proposed design reduces considerably the cost of the instrument and enhances remarkably its operative flexibility. Experiments were carried out at resolutions of 2.5, 5, 10, and 20 μm. The scanner detects 0.128 nmol L−1 carboxyfluorescein (spots with diameters of 70 μm) corresponding to 1.8 molecules μm−2. The linear range extends over 3.5 orders of magnitude ( R 2 = 0.997) and the dynamic range covers almost five orders of magnitude. DNA microarray model experiments were carried out, including staining with SYBR Green I and hybridization with oligonucleotides labeled with the fluorescent dyes Alexa 488, Alexa 594, and Alexa 633. [Figure not available: see fulltext.]
ACCESSION #
31342614

 

Related Articles

  • Amphipathic Properties of HIV-1 gp41 Fusion Inhibitors. Gochin, Miriam; Guangyanu Zho // Current Topics in Medicinal Chemistry;Dec2011, Vol. 11 Issue 24, p3022 

    Small molecule inhibition of HIV fusion has been an elusive goal, despite years of effort by both pharmaceutical and academic laboratories. In this review, we will discuss the amphipathic properties of both peptide and small molecule inhibitors of gp41-mediated fusion. Many of the peptides and...

  • Synthesis of bioorthogonal and crosslinking amino acids for use in peptide synthesis. Sundaram, G. S. M.; Morgan, Ian R.; Tippmann, Eric M. // Amino Acids;Nov2010, Vol. 39 Issue 5, p1381 

    The ability to incorporate non-canonical amino acids into proteins by genetic or chemical methods allows one to introduce novel chemical properties into a protein at a defined residue. Such a residue may then be modified using common organic transformations. In this way, the structure or...

  • Classification of A1- and A24-supertype molecules by analysis of their MHC-peptide binding repertoires. Sidney, John; Southwood, Scott; Sette, Alessandro // Immunogenetics;Jul2005, Vol. 57 Issue 6, p393 

    At the functional level, the majority of human leukocyte antigen (HLA) class I MHC variants can be classified into about ten different major groups, or supertypes, characterized by overlapping peptide binding motifs and repertoires. Previous studies have detailed the peptide binding specificity...

  • Yeast Genetic Methods for the Detection of Membrane Protein Interactions. Fetchko, Michael; Auerbach, Daniel; Stagljar, Igor // BioDrugs;2003, Vol. 17 Issue 6, p413 

    Focuses on the feasibility of using inhibitors of protein interactions as drugs and the adaptation of yeast genetic techniques to select for inhibitors of defined protein interactions. Monitoring of known protein interactions and uncovering of novel interactions by techniques such as the...

  • Methods for selection of aptamers to protein targets. Kulbachinskiy, A. V. // Biochemistry (00062979);Dec2007, Vol. 72 Issue 13, p1505 

    Aptamers are synthetic single-stranded RNA or DNA molecules capable of specific binding to other target molecules. In this review, the main aptamer properties are considered and methods for selection of aptamers against various protein targets are described. Special attention is given to the...

  • Structural insights into a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans. Ying Yang; Lulu Hu; Ping Wang; Haifeng Hou; Yan Lin; Yi Liu; Ze Li; Rui Gong; Xiang Feng; Lu Zhou; Wen Zhang; Yuhui Dong; Huirong Yang; Hanqing Lin; Yiqin Wang; Chen, Charlie Degui; Yanhui Xu // Cell Research;Aug2010, Vol. 20 Issue 8, p886 

    Histone lysine methylation can be removed by JmjC domain-containing proteins in a sequence- and methylation-state-specific manner. However, how substrate specificity is determined and how the enzymes are regulated were largely unknown. We recently found that ceKDM7A, a PHD- and JmjC...

  • Short-time dynamics of polypeptides. Arashiro, Everaldo; Drugowich de Felício, J. R.; Hansmann, Ulrich H. E. // Journal of Chemical Physics;1/28/2007, Vol. 126 Issue 4, p045107 

    The authors study the short-time dynamics of helix-forming polypeptide chains using an all-atom representation of the molecules and an implicit solvation model to approximate the interaction with the surrounding solvent. The results confirm earlier observations that the helix-coil transition in...

  • Protein interaction blockers. Sinclair, Meeghan // Nature Biotechnology;Aug2000, Vol. 18 Issue 8, p815 

    Discusses the development of a genetic selection method for searching peptide libraries for molecules that inhibit protein-protein interactions.

  • ORAL DELIVERY OF RECOMBINANT PRODUCTION OF PEPTIDE HORMONES PART II: RECOMBINANT PRODUCTION OF THERAPEUTIC PEPTIDES. Mehta, Nozer M. // BioPharm International;Jul2004, Vol. 17 Issue 7, p44 

    Part II. Describes a direct expression process for the oral delivery and recombinant production of peptide hormones. Ability of the technology to enable the development of orally delivered peptide hormone drugs for chronic administration; Issues with recombinant production of peptides;...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics