TITLE

Competitive protein adsorption to polymer surfaces from human serum

AUTHOR(S)
Holmberg, Maria; Stibius, Karin B.; Larsen, Niels B.; Xiaolin Hou
PUB. DATE
May 2008
SOURCE
Journal of Materials Science: Materials in Medicine;May2008, Vol. 19 Issue 5, p2179
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Surface modification by “soft” plasma polymerisation to obtain a hydrophilic and non-fouling polymer surface has been validated using radioactive labelling. Adsorption to unmodified and modified polymer surfaces, from both single protein and human serum solutions, has been investigated. By using different radioisotopes, albumin and Immunoglobulin G (IgG) adsorption has been monitored simultaneously during competitive adsorption processes, which to our knowledge has not been reported in the literature before. Results show that albumin and IgG adsorption is dependent on adsorption time and on the presence and concentration of other proteins in bulk solutions during adsorption. Generally, lower albumin and IgG adsorption was observed on the modified and more hydrophilic polymer surfaces, but otherwise the modified and unmodified polymer surfaces showed the same adsorption characteristics.
ACCESSION #
31333979

 

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