Competitive protein adsorption to polymer surfaces from human serum

Holmberg, Maria; Stibius, Karin B.; Larsen, Niels B.; Xiaolin Hou
May 2008
Journal of Materials Science: Materials in Medicine;May2008, Vol. 19 Issue 5, p2179
Academic Journal
Surface modification by “soft” plasma polymerisation to obtain a hydrophilic and non-fouling polymer surface has been validated using radioactive labelling. Adsorption to unmodified and modified polymer surfaces, from both single protein and human serum solutions, has been investigated. By using different radioisotopes, albumin and Immunoglobulin G (IgG) adsorption has been monitored simultaneously during competitive adsorption processes, which to our knowledge has not been reported in the literature before. Results show that albumin and IgG adsorption is dependent on adsorption time and on the presence and concentration of other proteins in bulk solutions during adsorption. Generally, lower albumin and IgG adsorption was observed on the modified and more hydrophilic polymer surfaces, but otherwise the modified and unmodified polymer surfaces showed the same adsorption characteristics.


Related Articles

  • Evaluation of water sorption property and in vitro blood compatibility of poly(2-hydroxyethyl methacrylate) (PHEMA) based semi interpenetrating polymer networks (IPNs). Bajpai, A. K.; Kankane, Sanjana // Journal of Materials Science: Materials in Medicine;May2008, Vol. 19 Issue 5, p1921 

    pH responsive smart biomaterials of gelatin and poly(2-hydroxyethyl methacrylate-co-acrylic acid) were synthesized by redox polymerization and characterized by FTIR, Environmental Scanning Electron Microscopy (ESEM). The prepared environmental responsive biomaterials containing polyelectrolyte...

  • Automated phosphorylation site mapping. Jensen, Ole N. // Nature Biotechnology;Oct2006, Vol. 24 Issue 10, p1226 

    The article presents the author's comments on the research conducted by S. A. Beausoleil and colleagues on improved computational techniques that facilitate for automated phosphorylation site assignment in large-scale proteomic experiments. Protein phosphorylation is arguably the most important...

  • New perspective in degradation mechanism of SrTiO3:Pr,Al,Ga phosphors. Jin Young Kim; Yong Chan You; Jong Hyuk Kang; Duk Young Jeon; Weber, Jörg // Journal of Materials Research;Sep2004, Vol. 19 Issue 9, p2694 

    Under prolonged electron-beam exposure, perovskite-structured SrTiO3:Pr,Al,Ga (STO) phosphor can be easily reduced due to oxygen loss. In particular, it is well known that dissociative H2O molecules are well adsorbed on reduced STO surfaces. The hydroxyl species produced by such dissociative...

  • Adsorption study of a commonly used antidepressant drug, fluoxetine hydrochloride, onto a crosslinked β-cyclodextrin-carboxymethylcellulose polymer. Bonenfant, Danielle; Mimeault, Murielle; Niquette, Patrick; Hausler, Robert // Water Science & Technology;2012, Vol. 66 Issue 1, p224 

    A study was carried out by ultraviolet-visible (UV-vis) and Fourier transform infrared (FTIR) spectroscopies to establish the efficiency of adsorption of fluoxetine hydrochloride (FLU), onto a crosslinked β-cyclodextrin-carboxymethylcellulose (β-CD-CMC) polymer. The adsorption was...

  • Protein and Dye Adsorption on Poly(ethylene terephthalate) Nucleopore Membranes Modified with Polymers. Mitrofanova, N. V.; Nechaev, A. N.; Khokhlova, T. D.; Mchedlishvili, B. V. // Colloid Journal;Mar/Apr2003, Vol. 65 Issue 2, p222 

    Adsorption of basic, neutral, and acidic proteins on poly(ethylene terephthalate) nucleopore membranes was studied as a function of solution pH. Higher adsorption values were observed for basic and neutral proteins and lower, for acidic proteins. The value of pH corresponding to maximal...

  • Layer-to-layer adsorption of oppositely charged polyelectrolytes: The effect of molecular mass: 1. Formation of the first layer. Sergeeva, I. P.; Ermakova, T. B.; Semenov, D. A.; Sobolev, V. D.; Kiseleva, O. A.; Churaev, N. V. // Colloid Journal;Sep2008, Vol. 70 Issue 5, p609 

    The formation and properties of adsorption layers of poly(dimethyldiallylammonium chloride) with different molecular masses on the surface of fused quartz are studied by the capillary electrokinetics method. It is shown that the value of ζ potential depends on the flow rate of liquid...

  • Thiocyanate separation by imprinted polymeric systems. Özkütük, Ebru Birlik; Özalp, Elif; Ersöz, Arzu; Açıkkalp, Erol; Say, Rıdvan // Microchimica Acta;Apr2010, Vol. 169 Issue 1/2, p129 

    Novel polymers for the specific recognition of thiocyanate ion (SCN) have been prepared by template polymerization of SCN with chitosan-Zn(II), the N-(2-aminoethyl)-3-aminopropyl-trimethoxysilan-Zn(II) complex, epichlorohydrin, and tetraethoxysilane. After removal of SCN, the imprinted beads...

  • Transglutaminases as Possible Therapeutic Targets in Neurodegenerative Diseases. Martin, Antonio; De Vivo, Giulia; Ricotta, Mariangela; Iannuzzi, Maura; Gentile, Vittorio // Recent Patents on CNS Drug Discovery;Nov2010, Vol. 5 Issue 3, p195 

    Transglutaminases are ubiquitous enzymes which catalyze post-translational modifications of proteins. The main activity of these enzymes is the cross-linking of glutaminyl residues of a protein/peptide substrate to lysyl residues of a protein/peptide co-substrate. In addition to lysyl residues,...

  • Interaction of PARP2 with DNA structures mimicking DNA repair intermediates. Kutuzov, M. M.; Ame, J.-C.; Khodyreva, S. N.; Schreiber, V.; Lavrik, O. I. // Biopolymers & Cell;Oct2011, Vol. 27 Issue 5, p383 

    Poly(ADP-ribosyl)ation is a posttranslational protein modification significant for the genomic stability and cell survival in response to DNA damage. Poly(ADP-ribosyl)ation is catalyzed by poly(ADP-ribose)polymerases (PARPs). Whereas the role of PARP1 in response to DNA damage has been widely...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics