TITLE

Comparison of Western blot to rapid anti HIV testing: Usefulness in confirming true positivity

AUTHOR(S)
Shastry, S.; Katharia, R.; Dubey, A.; Elhence, P.; Chaudhary, R. K.
PUB. DATE
January 2008
SOURCE
Asian Journal of Transfusion Science;Jan-Jun2008, Vol. 2 Issue 1, p27
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: The National blood policy now requires that blood donors positive for HIV infection be informed of their result and referred to voluntary counseling and testing center (VGTC) for further work up. Since ELISA may be false reactive due to many reasons, confirmation of results may be required before informing the donors and referring them to VGTC for further work up. Till now Western blot is the Gold Standard as a confirmatory test but the cost is high. In recent years rapid anti HIV test kits with excellent sensitivity and specificity have been developed and anti HIV ELISA reactive samples are tested by three different rapid kits at VGTGs to give confirmatory diagnosis. Adm: To compare the performance characteristics of Western blot and rapid test assays as confirmatory test for HIV. Materials and Methods: Over a study period of 1 year (August 2006 to August 2007) out of 18192 blood donations, 57 donor samples were repeat ELISA reactive (0.0031%). Of these 17 were reactive by third generation Elisa and 40 by fourth generation ELISA These were further tested with Western blot ( J.Mitra & Co. Ltd) and three rapid test assays (HIV-Coomb: J.Mitra & Co. Ltd, HIV Bidot: Diagnos Ltd and HIV 1.2.0 Triline test: Transasia Biomedicals Ltd). Statistical tests were applied using SPSS version 13. P-value of <0.05 was considered significant. Results: Out of 57 repeatedly ELISA reactive samples, 16 (28%) were Western blot positive. The same samples turned out to be rapid test positive showing that correlation of Western blot with rapid test positivity is 100% and true HIV positivity in our donor population as 0.00088%. 7/17 (41.17%) of IIIrd generation ELISA reactive samples were true positive in comparison to 9/40 (22.5%) of fourth generation ELISA reactive samples. Cost analysis revealed that rapid test assays are more cost-effective compared to Western blot assays.
ACCESSION #
31323478

 

Related Articles

  • Performance characteristics of serologic tests for human immunodeficiency virus type 1 (HIV-1) antibody among Minnesota blood donors. Public health and clinical implications. MacDonald, Kristine L.; Jackson, J. Brooks; Bowman, Robert J.; Polesky, Herbert F.; Rhame, Frank S.; Balfour Jr., Henry H.; Osterholm, Michael T.; MacDonald, K L; Jackson, J B; Bowman, R J; Polesky, H F; Rhame, F S; Balfour, H H Jr; Osterholm, M T // Annals of Internal Medicine;4/15/89, Vol. 110 Issue 8, p617 

    Study Objective: To evaluate performance characteristics of sequential enzyme immunoassay (EIA) and Western blot human immunodeficiency virus type 1 (HIV-1) antibody testing in a low-risk population.Design: Three-year prospective study of a selected sample from a...

  • Human T-cell lymphotropic virus in family members of seropositive blood donors: silent dissemination. Catalan-Soares, Bernadette; Carneiro-Proietti, Anna Bárbara; Proietti, Fernando Augusto // Revista Panamericana de Salud Publica;Dec2004, Vol. 16 Issue 6, p387 

    Objective. To investigate human T-cell lymphotropic virus transmission among family members of asymptomatic carriers identified through blood donor screening tests; and to determine the most likely direction of transmission in sexual partners having the same (concordant) serological diagnosis....

  • Anti-HIV-Pozitif Hastalarda DoÄŸrulama Testi Sonuçları: BeÅž Yılllık Verilerin lrdelenmesi. Yüksel, Pelin; Ziver, Tevhide; İzmirli, Sena; Aslan, Mustafa; Saribaş, Suat; Güngördü, Zeynep; Yakar, Hakan; Kocazeybek, Bekir // Klimik Journal / Klimik Dergisi;Aug2010, Vol. 23 Issue 2, p51 

    Objective: In this retrospective study, we aimed to discuss the results of anti-HIV-1/2 ELISA and Western-Blot (WB) tests, and particularly the distribution of HIV-specific seropositive bands performed in patients with suspected HIV infection and in blood donors and preoperative patients in the...

  • Elevation of Anti-Cytokeratin 18 Antibody and Circulating Cytokeratin 18: Anti-Cytokeratin 18 Antibody Immune Complexes in Sera of Patients with Idiopathic Pulmonary Fibrosis. Dobashi, N.; Fujita, J.; Murota, M.; Ohtsuki, Y.; Yamadori, I.; Yoshinouchi, T.; Ueda, R.; Bandoh, S.; Kamei, T.; Nishioka, M.; Ishida, T.; Takahara, J. // Lung;2000, Vol. 178 Issue 3, p171 

    Abstract. In this study, we hypothesize that anti-cytokeratin 18 (CK18) antibody and CK18:anti-CK18 immune complex increase in sera in patients with idiopathic pulmonary fibrosis (IPF). To prove the existence of anti-CK18 antibodies in patients' sera, bovine CK18 was stained with patients' sera...

  • Dried blood spots are an acceptable and useful HIV surveillance tool in a remote developing world setting. Solomon, S. S.; Pulimi, S.; Rodriguez, I. I.; Chaguturu, S. K.; Satish Kumar, S. K.; Mayer, K. H.; Solomon, S. // International Journal of STD & AIDS;Oct2004, Vol. 15 Issue 10, p658 

    Enzyme-linked immunosorbent assay and Western blot analysis of dried blood spots (DBS) on filter paper have been shown to be as sensitive and specific as analysis of serum, and therefore may be a cost-effective and culturally appropriate HIV seroprevalence tool in remote areas. This study...

  • Testing for Human Immunodeficiency Virus. Goederi, James J. // Annals of Internal Medicine;Oct86, Vol. 105 Issue 4, p609 

    Editorial. Deals with the testing for HIV infection. Effectiveness of the enzyme-linked immunosorbent assay (ELISA); Evaluation of Western blot testing; Steps to show whether ELISA reactivity is a true-positive or false-positive result.

  • HIV Infection with Negative Serological Tests: Development of Seropositivity in Association with Highly Active Antiretroviral Therapy. Chockalingam, Mirabai; Clarke, Lorraine; McCormack, William M. // AIDS Patient Care & STDs;Jun2000, Vol. 14 Issue 6, p305 

    A 19-year-old woman with well-documented HIV-1 infection had persistently negative enzyme immunoassay (EIA) and Western blot serological tests. She has plasma HIV-1 RNA levels of >480,000 copies/mL and T-helper cell counts of ~100/mm[sup 3]. When treated with highly active antiretroviral therapy...

  • Evidence for HTLV-III infection in prostitutes in Tamil Nadu (India). Simoes, Eric A. F.; Babu, P. George; John, T. Jacob; Nirmala, S.; Solomon, Suniti; Lakshminarayana, C. S.; Quinn, Thomas C. // Indian Journal of Medical Research;Oct2012, Vol. 136 Issue 4, p335 

    Antibody to human T-lymphotropic virus type III has been detected by the enzyme-linked immunosorbent assay and confirmed by western blot analysis in 10 among 102 prostitutes in Tamil Nadu (India).

  • Testing for HIV conversion. M.O.R. // Cortlandt Forum;03/25/98, Vol. 11 Issue 3, p103 

    Discusses the case of young woman who had an HIV conversion of a positive enzyme-linked immunosorbent assay (ELISA) to negative result in the Western blot test. Cause of the conversion of the HIV tests results; Difference between the ELISA and Western blot HIV tests; Non-infection of the...

Share

Read the Article

Courtesy of NEW JERSEY STATE LIBRARY

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics