TITLE

Disruption of gastric barrier function by luminal nitrosative stress: a potential chemical insult to the human gastro-oesophageal junction

AUTHOR(S)
Ara, N.; Iijima, K.; Asanuma, K.; Yoshitake, J.; Ohara, S.; Shimosegawa, T.; Yoshimura, T.
PUB. DATE
March 2008
SOURCE
Gut;Mar2008, Vol. 57 Issue 3, p306
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Objective: The human gastro-oesophageal junction is exposed to abundant amounts of luminal reactive nitrogen oxide species (RNOS) derived from the enterosalivary recirculation of dietary nitrate. The aim of this study is to investigate the direct effects of luminal RNOS on the adjacent gastric barrier function using an ex vivo chamber model. Methods: A chamber model in which the rat gastric mucosal membrane was mounted between the two halves of a chamber was designed to simulate the microenvironment of the lumen and the adjacent mucosa of the gastro-oesophageal junction. On the mucosal side of the chamber, RNOS were generated by the acidification of physiological concentrations of sodium nitrite. The epithelial barrier function was evaluated by electrophysiological transmembrane resistance, and membrane permeability with [³H]mannitol flux. The expression of occludin was evaluated by immunohistochemistry and immunoblotting. Dinitrosyl dithiolato iron complex (DNIC) was also measured by means of electron paramagnetic resonance spectroscopy to confirm the diffusion of RNOS from the mucosal lumen into the mounted mucosa. Results: The administration of acidified nitrite to the mucosal lumen caused both a decrease in transmembrane resistance and an increase in epithelial permeability, suggesting a disturbance of the gastric barrier function. These changes were accompanied by a derangement of the expression of occludin. The diffusion of luminal RNOS into the mounted membrane was confirmed by showing the generation of DNIC within the tissue. Conclusions: Simulating the microenvironment of the human gastro-oesophageal junction, this study demonstrated that RNOS generated luminally at the human gastro-oesophageal junction can derange the barrier function of the adjacent tissue by disrupting the tight junction.
ACCESSION #
31301013

 

Related Articles

  • Erratum.  // Allergy & Asthma Proceedings;Jan/Feb2013, Vol. 34 Issue 1, p107 

    A correction to the article "Assessment of Fractionated Exhaled Nitric Oxide as a Biomarker for the Treatment of Eosinophilic Esophagitis" that was published in the previous issue is presented.

  • IMPACT OF ALCOHOL ON RAT HEART AND ENDOTHELIAL CELLULAR NITRIC OXIDE SYNTHASE ACTIVITY IN VITRO AND IN VIVO. Yugandhar, M.; Sripathi, Babu; Vijuyudu; Kumari, K. V.; Rao, M. Rajeswara // International Journal of Pharmacology & Biological Sciences;Dec2013, Vol. 7 Issue 3, p9 

    Nitric oxide (NO) has been implicated in the regulation of heart contractility, platelet aggregation in the cardio vascular system of mammals. Multiple authors reported alcohol as to interact with cardio vascular NO pathway parameters. The impact of 20% ethanol (w/v) at selected doses of 5gm/per...

  • Expression of NADPH-d in the vagal nuclei of the chronic esophagitis model in rats. SEHIRLI, Ümit Süleyman; TUGTEPE, Halil; VERIMLI, Ural; KIRAZLI, Özlem; ÖZKAN, Mazhar; DAGLI, Emrullah Tolga // Turkish Journal of Medical Sciences;Apr2014, Vol. 44 Issue 2, p243 

    Aim: To evaluate the changes in the number of NADPH diaphorase (NADPH-d) stained neurons in the vagal nuclei in a chronic esophagitis model. Materials and methods: There were 3 groups of rats examined: 1) a chronic gastroesophageal reflux rat group, which was created by a partial gastric outlet...

  • Arginase attenuates inhibitory nonadrenergic noncholinergic nerve-induced nitric oxide generation and airway smooth muscle relaxation. Maarsingh, Harm; Tio, Marieke A.; Zaagsma, Johan; Meurs, Herman // Respiratory Research;2005, Vol. 6, p23 

    Background: Recent evidence suggests that endogenous arginase activity potentiates airway responsiveness to methacholine by attenuation of agonist-induced nitric oxide (NO) production, presumably by competition with epithelial constitutive NO synthase for the common substrate, Larginine. Using...

  • Inhibition or knock out of Inducible nitric oxide synthase result in resistance to bleomycin-induced lung injury. Genovese, Tiziana; Cuzzocrea, Salvatore; Di Paola, Rosanna; Failla, Marco; Mazzon, Emanuela; Sortino, Maria Angela; Frasca, Giuseppina; Gili, Elisa; Crimi, Nunzio; Caputi, Achille P.; Vancheri, Carlo // Respiratory Research;2005, Vol. 6, p58 

    Background: In the present study, by comparing the responses in wild-type mice (WT) and mice lacking (KO) the inducible (or type 2) nitric oxide synthase (iNOS), we investigated the role played by iNOS in the development of on the lung injury caused by bleomycin administration. When compared to...

  • The potent relaxant effect of resveratrol in rat corpus cavernosum and its underlying mechanisms. Dalaklioglu, S; Ozbey, G // International Journal of Impotence Research;Sep/Oct2013, Vol. 25 Issue 5, p188 

    The aim of this study was to evaluate the relaxant effect of resveratrol (RVT), one of the most commonly employed dietary polyphenols, in rat corpus cavernosum, and to further investigate the contribution of possible underlying mechanisms. Strips of corpus cavernosum were used in organ baths for...

  • A canine model of gastroesophageal reflux disease (GERD). McMahon, R.L.; Clary, E.M.; Eubanks, W.S. // Surgical Endoscopy;Feb2003, Vol. 17 Issue 2, p355 

    Comments on a canine model of gastroesophageal reflux disease (GERD). Observations that confirm previous experiences obtained in a pilot work that in the dog, surgical attenuation of the lower esophageal sphincter (LES) alone, either through longitudinal myotomy or subtotal myectomy, is...

  • A novel rat model to determine interaction between reflux oesophagitis and bronchial asthma. Sugawa, T.; Fujiwara, Y.; Yamagami, H.; Watanabe, K.; Tanigawa 1, T.; Shiba, M.; Watanabe, T.; Tominaga, K.; Oshitani, N.; Higuchi, K.; Arakawa, T. // Gut;May2008, Vol. 57 Issue 5, p575 

    BACKGROUND: Several studies have shown a strong association between reflux oesophagitis (RO) and bronchial asthma (BA). The precise mechanisms of interaction between RO and BA are uncertain, possibly due to lack of animal models. AIMS: We established a novel rat model and examined pathogenic...

  • Influence of Temporomandibular Joint Pain on Sleep Patterns: Role of Nitric Oxide. Schütz, T. C. B.; Andersen, M. L.; Tufik, S. // Journal of Dental Research;Sep2004, Vol. 83 Issue 9, p693 

    Since nitric oxide is related to nociception and the sleep-wake cycle, this study sought to determine its involvement in the altered sleep pattern in a temporomandibular joint pain model by investigating the effect of the inhibitor of nitric oxide synthase (L-NAME) and that of its precursor...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics