TITLE

Upregulation of matrix and adhesion molecules induced by controlled topography

AUTHOR(S)
Andrews, K. D.; Hunt, J. A.
PUB. DATE
April 2008
SOURCE
Journal of Materials Science: Materials in Medicine;Apr2008, Vol. 19 Issue 4, p1601
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Electrostatic spinning is receiving increasing attention in the field of tissue engineering, due to its ability to produce 3-dimensional, multidirectional, microfibrous scaffolds. These structures are capable of supporting a wide range of cell growth; however, there is little knowledge relating material substrates with specific cellular interactions and responses. The aim of this research was to investigate if electrostatically spun scaffolds, with controlled topographical features, would affect the adhesion mechanisms of contacting cells. A range of electrostatically spun Tecoflex® SG-80A polyurethane scaffolds was characterized in terms of inter-fibre separation, fibre diameter, surface roughness, void fraction and fibre orientation. Human embryonic lung fibroblasts and human vein endothelial cells were cultured on these scaffolds for 7, 14, 28 days, and analysed for their expression of extracellular matrix and adhesion molecules using image analysis and laser scanning confocal microscopy. There were significant differences in adhesion mechanisms between scaffolds, cell types and culture periods. Fibroblast-scaffolds were stimulated and oriented to a greater degree, and at earlier cultures, by the controlled topographical features than the endothelial cells. These conclusions confirm that cellular behaviour can be influenced by the induced scaffold topography at both molecular and cellular levels, with implications for optimum application specific tissue engineering constructs.
ACCESSION #
31244660

 

Related Articles

  • Integrin-mediated adhesion and stem-cell-niche interactions. Ellis, Stephanie J.; Tanentzapf, Guy // Cell & Tissue Research;Jan2010, Vol. 339 Issue 1, p121 

    The regulation of stem cell behavior and maintenance typically involves the integration of both intrinsic and extrinsic cues. One such external cue, integrin-mediated cell adhesion to the extracellular matrix, plays an important part in regulating stem cell function and maintenance. In...

  • Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering. Lutolf, M. P.; Hubbell, J. A. // Nature Biotechnology;Jan2005, Vol. 23 Issue 1, p47 

    New generations of synthetic biomaterials are being developed at a rapid pace for use as three-dimensional extracellular microenvironments to mimic the regulatory characteristics of natural extracellular matrices (ECMs) and ECM-bound growth factors, both for therapeutic applications and basic...

  • Changes in coagulation condition, cytokine, adhesion molecule after repair of type A aortic dissection. Nomura, F.; Tamura, K.; Yoshitatsu, M.; Katayama, A.; Katayama, K.; Ihara, K. // European Journal of Cardio-Thoracic Surgery;Aug2004, Vol. 26 Issue 2, p348 

    Objective: Because residual dissection often exists even after the repair of a type A dissection, we evaluated coagulation conditions, cytokine levels, and adhesion molecule levels in mid-term follow up after repair of type A dissections. Methods: Thrombin–antithrombin III complex (TAT),...

  • An lower bound on receptor density for stable cell adhesion due to thermal undulations. Yuan Lin; Freund, L. B. // Journal of Materials Science;Nov2007, Vol. 42 Issue 21, p8904 

    The adhesion of a living cell to an extracellular matrix surface is effected through the bonding of receptor molecules in the cell membrane to compatible ligand molecules on the surface. In a series of experiments on adhesion of cells to a substrate surface with a controlled density of ligand...

  • Integrins. Barczyk, Malgorzata; Carracedo, Sergio; Gullberg, Donald // Cell & Tissue Research;Jan2010, Vol. 339 Issue 1, p269 

    Integrins are cell adhesion receptors that are evolutionary old and that play important roles during developmental and pathological processes. The integrin family is composed of 24 αβ heterodimeric members that mediate the attachment of cells to the extracellular matrix (ECM) but that also...

  • Improvement of thermostability of recombinant collagen-like protein by incorporating a foldon sequence. Chunling Du; Mingqi Wang; Jinying Liu; Mingli Pan; Yurong Cai; Juming Yao // Applied Microbiology & Biotechnology;May2008, Vol. 79 Issue 2, p195 

    Collagen is a popular biomaterial in many specific biological interactions as well as a structural element. In this work, the recombinant collagen-like proteins were synthesized using Escherichia coli expression system. A foldon sequence, GYIPEAPRDGQAYVRKDGEWVLLSTFL, derived from the native T4...

  • Mineralised collagen–an artificial, extracellular bone matrix–improves osteogenic differentiation of bone marrow stromal cells. Bernhardt, Anne; Lode, Anja; Boxberger, Sabine; Pompe, Wolfgang; Gelinsky, Michael // Journal of Materials Science: Materials in Medicine;Jan2008, Vol. 19 Issue 1, p269 

    In the field of bone tissue engineering there is a high demand on bone graft materials which promote bone formation. By combination of collagen type I with nanocrystalline hydroxyapatite (HA) we generated a resorbable material which structure and composition is close to those of the...

  • Biocompatibility studies of human fetal osteoblast cells cultured on gamma titanium aluminide. Rivera-Denizard, Omayra; Diffoot-Carlo, Nannette; Navas, Vivian; Sundaram, Paul A. // Journal of Materials Science: Materials in Medicine;Jan2008, Vol. 19 Issue 1, p153 

    Ti-48Al-2Cr-2Nb (at. %) (γTiAl), a gamma titanium aluminide alloy originally designed for aerospace applications, appears to have excellent potential for bone repair and replacement. The biological response to γTiAl implant is expected to be similar to other titanium-based biomaterials....

  • Synergistic control of cell adhesion by integrins and syndecans. Morgan, Mark R.; Humphries, Martin J.; Bass, Mark D. // Nature Reviews Molecular Cell Biology;Dec2007, Vol. 8 Issue 12, p957 

    The ability of cells to adhere to each other and to their surrounding extracellular matrices is essential for a multicellular existence. Adhesion provides physical support for cells, regulates cell positioning and enables microenvironmental sensing. The integrins and the syndecans are two...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics