3D-Cultivation of bone marrow stromal cells on hydroxyapatite scaffolds fabricated by dispense-plotting and negative mould technique

Detsch, R.; Uhl, F.; Deisinger, U.; Ziegler, G.
April 2008
Journal of Materials Science: Materials in Medicine;Apr2008, Vol. 19 Issue 4, p1491
Academic Journal
The main principle of a bone tissue engineering (BTE) strategy is to cultivate osteogenic cells in an osteoconductive porous scaffold. Ceramic implants for osteogenesis are based mainly on hydroxyapatite (HA), since this is the inorganic component of bone. Rapid Prototyping (RP) is a new technology in research for producing ceramic scaffolds. This technology is particularly suitable for the fabrication of individually and specially tailored single implants. For tissue engineering these scaffolds are seeded with osteoblast or osteoblast precursor cells. To supply the cultured osteoblastic cells efficiently with nutrition in these 3D-geometries a bioreactor system can be used. The aim of this study was to analyse the influence of differently fabricated HA-scaffolds on bone marrow stromal cells. For this, two RP-techniques, dispense-plotting and a negative mould method, were used to produce porous ceramics. The manufactured HA-scaffolds were then cultivated in a dynamic system (bioreactor) with an osteoblastic precursor cell line. In our study, the applied RP-techniques give the opportunity to design and process HA-scaffolds with defined porosity, interconnectivity and 3D pore distribution. A higher differentiation of bone marrow stromal cells could be detected on the negative mould fabricated scaffolds, while cell proliferation was higher on the dispense-plotted scaffolds. Nevertheless, both scaffold types can be used in tissue engineering applications.


Related Articles

  • Cytocompatibility of calcium phosphate coatings deposited by an ArF pulsed laser. Hashimoto, Y.; Kawashima, M.; Hatanaka, R.; Kusunoki, M.; Nishikawa, H.; Hontsu, S.; Nakamura, M. // Journal of Materials Science: Materials in Medicine;Jan2008, Vol. 19 Issue 1, p327 

    In the current studies, we deposited ultra-thin hydroxyapatite films on a pure titanium substrate by pulsed laser deposition, and we examined the effects of these surfaces on rat bone marrow (RBM) cells. This method allowed deposition of 500-, 2,000-, and 5,000-Ã…-thick hydroxyapatite films....

  • Porous polymer/hydroxyapatite scaffolds: characterization and biocompatibility investigations. Douglas, Timothy; Pamula, Elzbieta; Hauk, Dominik; Wiltfang, Jörg; Sivananthan, Sureshan; Sherry, Eugene; Warnke, Patrick H // Journal of Materials Science: Materials in Medicine;Sep2009, Vol. 20 Issue 9, p1909 

    Poly-lactic-glycolic acid (PLGA) has been widely used as a scaffold material for bone tissue engineering applications. 3D sponge-like porous scaffolds have previously been generated through a solvent casting and salt leaching technique. In this study, polymer–ceramic composite scaffolds...

  • Fabrication of porous polycaprolactone/hydroxyapatite (PCL/HA) blend scaffolds using a 3D plotting system for bone tissue engineering. Park, Su A.; Su Hee Lee; Wan Doo Kim // Bioprocess & Biosystems Engineering;May2011, Vol. 34 Issue 4, p505 

    For tissue engineering and regeneration, a porous scaffold with interconnected networks is needed to guide cell attachment and growth/ingrowth in three-dimensional (3D) structure. Using a rapid prototyping (RP) technique, we designed and fabricated 3D plotting system and three types of...

  • Mechanical properties and cytocompatibility of biomimetic hydroxyapatite-gelatin nanocomposites. Ching-Chang Ko; Oyen, Michelle; Fallgatter, Alison M.; Jin-Hong Kim; Fricton, Jim; Wei-Shou Hu // Journal of Materials Research;Dec2006, Vol. 21 Issue 12, p17 

    A hydroxyapatite-gelatin nanocomposite system has been developed to resemble the composition and ultrastructure of natural bone for the application of tissue engineering. In the current study, variations in composition-content of gelatin and glutaraldehyde crosslinker-were examined in the...

  • Production and Mechanical Properties of Commercial Synthetic Hydroxyapatite (CSHA) Composites. Demirkol, Nermin; Oktar, Faik Nuzhet; Kayali, Eyup Sabri // Key Engineering Materials;2014, Vol. 587, p147 

    Hydroxyapatite (HA), one of the calcium phosphate compounds, is the most widely used bioceramic. HA materials have a common usage in bone repairing due to its ability to accelerate the bone growth around the implant. HA is a biocompatible material and used in production of various kinds of...

  • Mechanochemical synthesis evaluation of nanocrystalline bone-derived bioceramic powder using for bone tissue engineering. Khandan, Amirsalar; Karamian, Ebrahim; Bonakdarchian, Morteza // Dental Hypotheses;Oct-Dec2014, Vol. 5 Issue 4, p155 

    Introduction: Bone tissue engineering proposes a suitable way to regenerate lost bones. Different materials have been considered for use in bone tissue engineering. Hydroxyapatite (HA) is a significant success of bioceramics as a bone tissue repairing biomaterial. Among different bioceramic...

  • Response of stem cells from different origins to biphasic calcium phosphate bioceramics. Lobo, Sonja; Glickman, Robert; Silva, Wagner; Arinzeh, Treena; Kerkis, Irina // Cell & Tissue Research;Aug2015, Vol. 361 Issue 2, p477 

    Biphasic calcium phosphate (BCP) bioceramics have been successfully applied in a broad variety of presentation forms and with different ratios of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP). BCPs have been loaded with stem cells from different origins for bone tissue engineering...

  • In vitro osteoinductive potential of porous monetite for bone tissue engineering. Idowu, Bernadine; Cama, Giuseppe; Deb, Sanjukta; Di Silvio, Lucy // Journal of Tissue Engineering;Jan-Dec2014, Vol. 5, p1 

    Tissue engineering-based bone grafts are emerging as a viable alternative treatment modality to repair and regenerate tissues damaged as a result of disease or injury. The choice of the biomaterial component is a critical determinant of the success of the graft or scaffold; essentially, it must...

  • Flow Cytometric Cell Sorting and In Vitro Pre-Osteoinduction Are Not Requirements for In Vivo Bone Formation by Human Adipose-Derived Stromal Cells. Liu, Yunsong; Zhao, Yan; Zhang, Xiao; Chen, Tong; Zhao, Xianghui; Ma, Gui-e; Zhou, Yongsheng // PLoS ONE;Feb2013, Vol. 8 Issue 2, p1 

    Human adipose-derived stromal cells (hASCs) are a promising cell source for bone tissue engineering. However, before the clinical application of hASCs for the treatment of bone defects, key questions require answers, including whether pre-osteoinduction (OI) and flow cytometric cell purification...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics