Cell type-specific aspects in biocompatibility testing: the intercellular contact in vitro as an indicator for endothelial cell compatibility

Peters, Kirsten; Unger, Ronald E.; Stumpf, Susanne; Schäfer, Julia; Tsaryk, Roman; Hoffmann, Bettina; Eisenbarth, Eva; Breme, Jürgen; Ziegler, Günter; Kirkpatrick, C. J.
April 2008
Journal of Materials Science: Materials in Medicine;Apr2008, Vol. 19 Issue 4, p1637
Academic Journal
Endothelial cells cover the inner surface of blood vessels and form the interface between the blood and the tissues. Endothelial cells are involved in regulating barrier function, which is maintained by the interendothelial cell contacts. These interendothelial cell contacts are established by the interaction of different molecules. The maintenance of the barrier requires an appropriate signalling between these molecules. Thus, a number of different signalling pathways are integrated within interendothelial contacts. Since endothelial cells are important in tissue-implant interactions (especially for stent materials) this study examines the expression pattern of different interendothelial contact molecules to determine the usefulness in the analysis of biocompatibility in vitro. The effects of different pro-inflammatory and toxic stimuli and contact of human microvascular endothelial cells to metallic surfaces were examined for their impact on the pattern of interendothelial contact molecules. Striking modifications in the arrangement of these molecules were induced and the mode of modification was dependent on the tested compound. Thus, examining the pattern of expression of specific interendothelial contact molecules in vitro may be useful for testing the endothelial cell compatibility of biomaterials and their corrosion products.


Related Articles

  • In vivo and in vitro response to electrochemically anodized Ti-6Al-4V alloy. Yu Mi Lee; Eun Jung Lee; Sung Tae Yee; Byung Il Kim; Eun Sang Choe; Hyun Wook Cho // Journal of Materials Science: Materials in Medicine;May2008, Vol. 19 Issue 5, p1851 

    Tissues’ reactions to metals depend on a variety of properties of the metal, most notably surface structure. Anodizing has been shown to alter the surface properties of metal, thus eliciting a change in the biocompatibility of the metal . In order to evaluate the biocompatibility of...

  • I Kr contributes to the altered ventricular repolarization that determines long-term cardiac memory. Obreztchikova, Maria N.; Patberg, Kornelis W.; Plotnikov, Alexei N.; Ozgen, Nazira; Shlapakova, Irina N.; Rybin, Andrew V.; Sosunov, Eugene A.; Danilo, Peter; Anyukhovsky, Evgeny P.; Robinson, Richard B.; Rosen, Michael R. // Cardiovascular Research;Jul2006, Vol. 71 Issue 1, p88 

    Abstract: Objective: Cardiac memory (CM) is characterized by an altered T-wave morphology, which reflects altered repolarization gradients. We hypothesized that the delayed rectifier currents, I Kr and I Ks, might contribute to these repolarization changes. Methods: We studied conscious,...

  • Ectopic expression of Arabidopsis CYCD2 and CYCD3 in tobacco has distinct effects on the structural organization of the shoot apical meristem. Boucheron, E.; Healy, J. H. S.; Bajon, C.; Sauvanet, A.; Rembur, J.; Noin, M.; Sekine, M.; Khamlichi, C. Riou; Murray, J. A. H.; Van Onckelen, H.; Chriqui, D. // Journal of Experimental Botany;Jan2005, Vol. 56 Issue 409, p123 

    Transgenic tobacco lines expressing Arath-CYCD2 or Arath-CYCD3 genes under a cauliflower mosaic virus 35S promoter are modified in the timing of their development, but not in the phenotype of their vegetative organs. They display an increased rate of leaf initiation, which is shown to be...

  • Alginate hydrogel as a promising scaffold for dental-derived stem cells: an in vitro study. Moshaverinia, Alireza; Chen, Chider; Akiyama, Kentaro; Ansari, Sahar; Xu, Xingtian; Chee, Winston; Schricker, Scott; Shi, Songtao // Journal of Materials Science: Materials in Medicine;Dec2012, Vol. 23 Issue 12, p3041 

    The objectives of this study were to: (1) develop an injectable and biodegradable scaffold based on oxidized alginate microbeads encapsulating periodontal ligament (PDLSCs) and gingival mesenchymal stem cells (GMSCs); and (2) investigate the stem cell viability, and osteogenic differentiation of...

  • The bHLH transcription factor SPATULA regulates root growth by controlling the size of the root meristem. Makkena, Srilakshmi; Lamb, Rebecca S. // BMC Plant Biology;2013, Vol. 13 Issue 1, p1 

    Background: The Arabidopsis thaliana gene SPATULA (SPT), encoding a bHLH transcription factor, was originally identified for its role in pistil development. SPT is necessary for the growth and development of all carpel margin tissues including the style, stigma, septum and transmitting tract....

  • Efficient method for slow cryopreservation of human embryonic stem cells in xeno-free conditions. Valbuena, Diana; Sánchez-Luengo, Silvia; Galán, Amparo; Sánchez, Eva; Gómez, Eva; Poo, M. Eugenia; Ruiz, Verónica; Genbacev, Olga; Krtolica, Ana; Pellicer, Antonio; Moreno, Rubén; Simón, Carlos // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Jul2008, Vol. 17 Issue 1, p127 

    An effective, consistent and xeno-free cryopreservation technique is crucial for any human embryonic stem cell (hESC) laboratory with future perspectives for clinical application. This study presents a new slow freezing--rapid thawing method in serum-free conditions that allows the...

  • Sperm quality and cryopreservation of Brazilian freshwater fish species: a review. Viveiros, A. T. M.; Godinho, H. P. // Fish Physiology & Biochemistry;Mar2009, Vol. 35 Issue 1, p137 

    The Brazilian freshwater fish diversity is the richest in the world. Only 0.7% of all Brazilian species have had any aspect of their sperm biology addressed up to this date. The majority of the fish species described in this review migrate during the spawning season (a phenomenon known as...

  • Efficiency of application original preservatives for conservation leukocytes at -40 °C. Zaytseva, O. O.; Polejaeva, T. V.; Svedentsov, E. P; Solomina, O. N; Utemov, S. V. // Journal of Stress Physiology & Biochemistry;2011, Vol. 7 Issue 4, p197 

    When frozen leukocytes exposed to harmful factors of the complex, due to their complex cellular structure and high metabolism. Cryopreservatives allow to avoid damages, but most of which are toxic. The aim of the present was to compare the efficacy of application of two non-toxic solutions for...

  • Affinity for, and localization of, PEG-functionalized silica nanoparticles to sites of damage in an ex vivo spinal cord injury model. Chen, Bojun; Zuberi, Mahvash; Ben Borgens, Richard; Youngnam Cho // Journal of Biological Engineering;2012, Vol. 6 Issue 1, p18 

    Background: Traumatic spinal cord injury (SCI) leads to serious neurological and functional deficits through a chain of pathophysiological events. At the molecular level, progressive damage is initially revealed by collapse of plasma membrane organization and integrity produced by breaches....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics