TITLE

Behaviors of keratinocytes and fibroblasts on films of PLA50–PEO–PLA50 triblock copolymers with various PLA segment lengths

AUTHOR(S)
Garric, Xavier; Garreau, Henri; Vert, Michel; Molès, Jean-Pierre
PUB. DATE
April 2008
SOURCE
Journal of Materials Science: Materials in Medicine;Apr2008, Vol. 19 Issue 4, p1645
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The growth of human primary keratinocytes and fibroblasts on PLA–PEO–PLA copolymer films was investigated as an intermediate stage of a strategy aimed at making implantable dermo-epidermal substitutes. Four PLA–PEO–PLA triblock copolymers with the same PEO block and different dl-lactic acid/ethylene oxide molar ratios (LA/EO) (0.8, 1.4, 1.8 and 2), were synthesized and characterized by 1H-nuclear magnetic resonance and infrared spectroscopy. The films made of these copolymers were more hydrophilic than PLA50 and than tissue culture polystyrene controls according to contact angles with water. Proliferation and adhesion of human skin cells were evaluated by MTT assay and by scanning electron microscopy. The presence of PEO in the triblock copolymers influenced cell adhesion and proliferation of fibroblasts, whereas keratinocyte adhesion and proliferation were not affected. These features emphasize the interest of PLA–PEO–PLA triblock copolymers to serve as better compounds than the racemic PLA previously investigated to make supports for human skin primary cells and scaffolds for skin engineering.
ACCESSION #
31244629

 

Related Articles

  • Glycan Dependence of Galectin-3 Self-Association Properties. Halimi, Hubert; Rigato, Annafrancesca; Byrne, Deborah; Ferracci, Géraldine; Sebban-Kreuzer, Corinne; ElAntak, Latifa; Guerlesquin, Francoise // PLoS ONE;Nov2014, Vol. 9 Issue 11, p1 

    Human Galectin-3 is found in the nucleus, the cytoplasm and at the cell surface. This lectin is constituted of two domains: an unfolded N-terminal domain and a C-terminal Carbohydrate Recognition Domain (CRD). There are still uncertainties about the relationship between the quaternary structure...

  • The influence of pore size on colonization of poly( l-lactide-glycolide) scaffolds with human osteoblast-like MG 63 cells in vitro. Pamula, Elzbieta; Bacakova, Lucie; Filova, Elena; Buczynska, Joanna; Dobrzynski, Piotr; Noskova, Lenka; Grausova, Lubica // Journal of Materials Science: Materials in Medicine;Jan2008, Vol. 19 Issue 1, p425 

    A degradable copolymer of l-lactide and glycolide (PLG) was synthesized by ring opening polymerization using zirconium acetylacetonate [Zr(acac)4] as a biocompatible initiator. The structure of the copolymer was studied by nuclear magnetic resonance spectroscopy (NMR) and gel permeation...

  • Synthesis and Bioactivity of Polymer‐Based Synthetic Mimics of Antimicrobial Peptides (SMAMPs) Made from Asymmetrically Disubstituted Itaconates. Boschert, David; Schneider‐chaabane, Alexandra; Himmelsbach, Andreas; Eickenscheidt, Alice; Lienkamp, Karen // Chemistry - A European Journal;6/7/2018, Vol. 24 Issue 32, p8217 

    Abstract: A series of asymmetrically disubstituted diitaconate monomers is presented. Starting from itaconic anhydride, functional groups could be placed selectively at the two nonequivalent carbonyl groups. By using 2D NMR spectroscopy, it was shown that the first functionalization step...

  • News in brief.  // Nature Methods;Apr2010, Vol. 7 Issue 4, p255 

    The article offers news briefs related to the biological researches. The researcher Thomas Vierbuchen demonstrated a way to directly convert fibroblasts into distantly related excitatory neurons. Cis-regulatory modules (CRM) controls gene expressions as parts of complex regulatory networks. In a...

  • Odd behavior of giant quadrupole resonance.  // Physics Today;Jan75, Vol. 28 Issue 1, p18 

    Focuses on the behavior of a giant electric quadrupole resonance in nuclear spectra. Properties; Details on the resonance's mass number; Significance of radiative-capture experiments.

  • Parallel Imaging Artifacts in Body Magnetic Resonance Imaging. Noël, Patricia; Bammer, Roland; Reinhold, Caroline; Haider, Masoom A. // Canadian Association of Radiologists Journal;Apr2009, Vol. 60 Issue 2, p91 

    Objective: To familiarize the reader with the fundamental concepts of partial parallel imaging (PPI); to review the technical aspects of PPI including calibration scan, coil geometry, and field of view (FOV); and to illustrate artifacts related to parallel imaging and describe solutions to...

  • Synthesis and characterization of linear polylactic acid-based urethanes using Tin modified solid cloisite-30B catalyst. Selukar, Balaji S.; Parwe, Sharad P.; Mohite, Kavita K.; Garnaik, Baijayantimala // Advanced Materials Letters;Apr2012, Vol. 3 Issue 2, p161 

    In recent year, biocompatible, biodegradable materials (linear polylactic acid-based urethanes) in the solid and/or liquid form have attracted tremendous attention in biomedical application particularly in stent coating. Low molecular weight poly (lactic acid) s (PLA), having narrow molecular...

  • MAS-NMR studies of geopolymers heat-treated for applications in biomaterials field. Oudadesse, H.; Derrien, A.; Lefloch, M.; Davidovits, J. // Journal of Materials Science;May2007, Vol. 42 Issue 9, p3092 

    In the field of biomaterials applied in bony restoration, systems based on amorphous silicate network present the ability to link to bone matrix. Amorphous geopolymers of the potassium-poly(sialate)-nanopolymer type with a mole ratio Si:Al = 31 were studied for their use as potential...

  • Feasibility study for the rapid screening of target molecules using translational diffusion coefficients: diffusion-ordered NMR spectroscopy of biological toxins. Henderson, Terry J. // Analytical & Bioanalytical Chemistry;Feb2010, Vol. 396 Issue 4, p1465 

    A panel of 15 biological toxins ranging between ~60–28,000 g/mol was used to evaluate the feasibility of screening aqueous samples for toxin analytes based on their translational diffusion coefficients, Dt. Toxin Dt values were measured by pulsed-field gradient 1H NMR spectroscopy using a...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics