TITLE

The effect of adsorbed vitamin D and K to hydroxyapatite on ALP activity of MC3T3-E1 cell

AUTHOR(S)
Ozeki, K.; Aoki, H.; Fukui, Y.
PUB. DATE
April 2008
SOURCE
Journal of Materials Science: Materials in Medicine;Apr2008, Vol. 19 Issue 4, p1753
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
This study describes the adsorptive property of vitamins on HA and the effect of the HA adsorbed vitamin on the alkaline phosphatase (ALP) activity for effective use as a bone graft substitute. The vitamins used were calciferol (D3), menaquirone (K2) and 25-hydroxycholecalciferol (25(OH)D3). These vitamins were adsorbed on HA at 4, 10, 20, 37 and 50 °C. The adsorption amount was constant below 20 °C, and decreased as the incubation temperature increased over 20 °C. The order of the adsorption amount was: 25(OH)D3 > K2 > D3. The HA adsorbed vitamins (HA/D3, HA/25(OH)D3 and HA/K2) were suspended in physiological saline for 48 h for the release test. The release ratio of all vitamins increased with incubation time. The order of the release ratio was: 25(OH)D3 > K2 > D3, which was proportional to that of the adsorption amount. The ALP activity of MC3T3-E1 osteoblast-like cells on HA, HA/D3, HA/25(OH)D3 and HA/K2 was also investigated. The ALP activity was higher on HA/25(OH)D3 than on any other samples. However, HA/K2 and HA/D3 showed similar ALP activity to HA.
ACCESSION #
31244625

 

Related Articles

  • The effect of composition on ion release from Ca—Sr—Na—Zn—Si glass bone grafts. Murphy, S.; Boyd, D.; Moane, S.; Bennett, M. // Journal of Materials Science: Materials in Medicine;Nov2009, Vol. 20 Issue 11, p2207 

    Controlled delivery of active ions from biomaterials has become critical in bone regeneration. Some silica-based materials, in particular bioactive glasses, have received much attention due to the ability of their dissolution products to promote cell proliferation, cell differentiation and...

  • THE IMPACT OF AUGMENTATION MATERIALS ON THE RECEIVER BONES. Papakoca, Kiro; Neamtu, Raluca; Dimitriu, Bogdan; Drafta, Sergiu; Mihai, Augustin // Metalurgia International;May2010 Supplement, p229 

    The use of different types of materials for bone augmentation has generated lots of controversies regarding the formation of the new bone substrata. Starting with the autogen bone, continuing with ceramic, plastic masses and finishing with metals, all these materials lead to a process of...

  • Vitamin D deficiency and its correction in children with sickle cell anaemia. Wykes, Clare; Arasaretnam, Anita; O'Driscoll, Sandra; Farnham, Laura; Moniz, Caje; Rees, David // Annals of Hematology;Dec2014, Vol. 93 Issue 12, p2051 

    Vitamin D deficiency is common in sickle cell anaemia (SCA, HbSS), although its significance and optimal means of correction are unknown. We conducted an audit to assess the clinical significance of 25-hydroxy vitamin D (25-OHD) deficiency in children with SCA and to evaluate two methods of...

  • The biodegradation of hydroxyapatite bone graft substitutes in vivo. Rumpel, E.; Wolf, E.; Kauschke, E.; Bienengräber, V.; Bayerlein, T.; Gedrange, T.; Proff, P. // Folia Morphologica;2006, Vol. 65 Issue 1, p43 

    Hydroxyapatite (HA) ceramics are widely used for bone reconstruction. They are osteoconductive and serve as structural scaffolds for the deposition of new bone. Generally, scaffold materials should be degradable as they affect the mechanical properties of the reconstructed bone negatively....

  • Mechanical properties of morcellised bone graft with the addition of hydroxyapatite. McNamara, I.; Howard, J.; Rayment, A.; Schalk, R.; Brooks, R.; Best, S.; Rushton, N. // Journal of Materials Science: Materials in Medicine;Feb2014, Vol. 25 Issue 2, p321 

    Mixtures of morcellised bone graft (MBG) and hydroxyapatite (HA) are frequently used in revision arthroplasty surgery. However, the changes in the mechanical properties from adding HA to MBG are unknown. This study used a uniaxial compression test to replicate impaction bone grafting and...

  • The Spreader-Splay Graft Combination: A Treatment Approach for the Osseocartilaginous Vault Deformities Following Rhinoplasty. Acartürk, Sabri; Gencel, Eyüphan // Aesthetic Plastic Surgery;Aug2003, Vol. 27 Issue 4, p275 

    Deformities following primary rhinoplasty may be located at different anatomical regions related to the primary operation. Osseocartilaginous vault deformities such as open roof deformity, over-resected bony and cartilaginous dorsum, excessive width of the middle vault, inverted-V deformity and...

  • A Small Animal Model for Investigating Endosseous Dental Implants: Effect of Graft Materials on Healing of Endosseous, Porous-Surfaced Implants Placed in a Fresh Extraction Socket. Todescan Jr., Reynaldo; Pilliar, Robert M.; Melcher, Antony H. // International Journal of Oral & Maxillofacial Implants;Winter1987, Vol. 2 Issue 4, p76 

    A small animal model to investigate materials and implant designs intended for use as immediate replacements for extracted teeth has been developed. The effect of biological graft materials such as mineralized bone powder, demineralized bone powder, and collagen (Zyderm) on healing in relation...

  • Comparative ultrastructure evaluation of particle biomaterial of ovine origin. Zielak, João César; Locatelli, Patrícia; Pozzan, Sônia; Giovanini, Allan Fernando; Deliberador, Tatiana Miranda; de Andrade Urban, Cícero; Baratto-Filho, Flares // RSBO: Revista Sul-Brasileira de Odontologia;jan-mar2011, Vol. 8 Issue 1, p36 

    Introduction: Xenogenic bone grafting biomaterials have been vastly studied. Easy clinical use and low cost have attracted professionals and patients. Alternatively to bovine biomaterial, ovine biomaterial can be developed. Objective: To compare by scanning electron microscopy the ultrastructure...

  • BIPHASIC CERAMICS AND FIBRIN SEALANT FOR BONE RECONSTRUCTION IN EAR SURGERY. D'Arc, Maurice Bagot; Daculsi, Guy; Emam, Nadya // Annals of Otology, Rhinology & Laryngology;Sep2004, Vol. 113 Issue 9, p711 

    reconstruction is still a matter of concern in middle ear surgery despite the large number of surgical techniques proposed. A composite made of biphasic calcium phosphate ceramic granules mixed with human fibrin sealant, forming a moldable material that is easy to set for bone reconstruction,...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics