TITLE

Simultaneous determination of glutathione and cysteine concentrations and 2H enrichments in microvolumes of neonatal blood using gas chromatography–mass spectrometry

AUTHOR(S)
Küster, Alice; Tea, Illa; Sweeten, Shawn; Rozé, Jean-Christophe; Robins, Richard J.; Darmaun, Dominique
PUB. DATE
March 2008
SOURCE
Analytical & Bioanalytical Chemistry;Mar2008, Vol. 390 Issue 5, p1403
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A method is described whereby the concentrations and 2H isotope enrichment of glutathione (GSH) and cysteine can be simultaneously determined in a single gas chromatography–mass spectrometry run following derivatization as their N, S-ethoxycarbonyl methyl esters. Improvements of the derivatization protocol and the use of a short gas chromatography column combined with single-ion monitoring allow for rapid quantification of these parameters with acceptable precision and reproducibility (coefficient of variation less than 5%). The method makes possible their quantitative measurement in very small volumes (50 μL) of human umbilical cord blood, and is thus suitable for determining the cysteine and GSH concentrations and 2H isotope enrichments in blood samples from neonates or in other conditions in which sample size is restricted. It is shown that the fractional synthesis rate of human umbilical erythrocyte lysates in vitro is several-fold greater than that measured in vivo.
ACCESSION #
30055806

 

Related Articles

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics