TITLE

WPŁYW WYBRANYCH LEKÓW BISFOSFONIANOWYCH NA BŁONĘ ERYTROCYTÓW CZŁOWIEKA

AUTHOR(S)
Kopka, Anna; Bukowska, Bożena; Szadkowska, Bożena; Marczak, Agnieszka; Duda, Wirgiliusz
PUB. DATE
December 2007
SOURCE
Problemy Terapii Monitorowanej;dec2007, Vol. 18 Issue 4, p201
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Bisphosphonates are a group of synthetic substances that are derivatives of pirophosphonic acid, in which the atom of oxygen was introduced into pirophosphoran molecule in the place of carbon atom. These substances are resistant to the activities of phosphatases which enables their storage in bones and keeping therapeutic concentration. The affinity of bisphosphonates to the mineral, nascent bone tissue leads to deposition of these compounds in places of active bone restructure where they stop excessive resorption caused by osteoclast. When these medicines are administrated into alimentary tract they may cause very strong side effects. Taking the medicines during the meals leads to depletion of their absorption, and that is why it is necessary to take them before morning meal. Administrating the new, weekly doses, taken once a week, seems to have similar result and it limits the intensity of side effects. The best promising result seems to be the application of oral forms of bisphosphonates. This form of therapy may be the promise method of osteoporosis treatment in future, however it is hard to estimate what side effects may appear in the result of such treatment. That is why, it is advisable to examine the side effects of bisphosphonates an human erythrocytes. In this study, we continued our investigations on the interaction of bisphosphonates with human erythrocytes and now we focused our researches on the effect of these compounds on the fluidity erythrocyte membrane and the activity of ATPase. In the work, two groups of bisphosphonates have been investigated: disodium etidronate (19 - 3581 µM) and sodium alendronate (0,33 - 100 µM). The fluorometric method and fluorescent probes (ANS, DPH and TMA -- DPH) were used to estimate the fluidity of erythrocyte membrane. Additionally, an analysis of the disturbances of erythrocytes size and shape, accompanied by the application of flow cytometry was performed.
ACCESSION #
29366383

 

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