TITLE

Crystal structure of subunit VPS25 of the endosomal trafficking complex ESCRT-II

AUTHOR(S)
Wernimont, Amy K.; Weissenhorn, Winfried
PUB. DATE
January 2004
SOURCE
BMC Structural Biology;2004, Vol. 4, p10
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Down-regulation of plasma membrane receptors via the endocytic pathway involves their monoubiquitylation, transport to endosomal membranes and eventual sorting into multi vesicular bodies (MVB) destined for lysosomal degradation. Successive assemblies of Endosomal Sorting Complexes Required for Transport (ESCRT-I, -II and III) largely mediate sorting of plasma membrane receptors at endosomal membranes, the formation of multivesicular bodies and their release into the endosomal lumen. In addition, the human ESCRT-II has been shown to form a complex with RNA polymerase II elongation factor ELL in order to exert transcriptional control activity. Results: Here we report the crystal structure of Vps25 at 3.1 Ã… resolution. Vps25 crystallizes in a dimeric form and each monomer is composed of two winged helix domains arranged in tandem. Structural comparisons detect no conformational changes between unliganded Vps25 and Vps25 within the ESCRT-II complex composed of two Vps25 copies and one copy each of Vps22 and Vps36 [1,2]. Conclusions: Our structural analyses present a framework for studying Vps25 interactions with ESCRT-I and ESCRT-III partners. Winged helix domain containing proteins have been implicated in nucleic acid binding and it remains to be determined whether Vps25 has a similar activity which might play a role in the proposed transcriptional control exerted by Vps25 and/or the whole ESCRT-II complex.
ACCESSION #
29323554

 

Related Articles

  • AMPH-1/Amphiphysin/Bin1 functions with RME-1/Ehd1 in endocytic recycling. Pant, Saumya; Sharma, Mahak; Patel, Kruti; Caplan, Steve; Carr, Chavela M.; Grant, Barth D. // Nature Cell Biology;Dec2009, Vol. 11 Issue 12, p1399 

    RME-1/EHD1 (receptor mediated endocytosis/Eps15 homology-domain containing 1) family proteins are key residents of the recycling endosome, which are required for endosome-to-plasma membrane transport in Caenorhabditis elegans and mammals. Recent studies suggest similarities between the RME-1/EHD...

  • The plasma membrane-bound phospholipase Dδ enhances freezing tolerance in Arabidopsis thaliana. Li, Weiqi; Li, Maoyin; Zhang, Wenhua; Welti, Ruth; Wang, Xuemin // Nature Biotechnology;Apr2004, Vol. 22 Issue 4, p427 

    Freezing injury is a major environmental limitation on the productivity and geographical distribution of plants. Here we show that freezing tolerance can be manipulated in Arabidopsis thaliana by genetic alteration of the gene encoding phospholipase Dd (PLDd), which is involved in membrane lipid...

  • FOXO1 Up-Regulates Human L-selectin Expression Through Binding to a Consensus FOXO1 Motif. Yuefen Lou; Xiaojiong Lu; Xitong Dang // Gene Regulation & Systems Biology;2012, Issue 6, p139 

    L-selectin plays important roles in lymphocyte homing and leukocyte rolling. Mounting evidence shows that it is involved in many disease entities including diabetes, ischemia/reperfusion injuries, inflammatory diseases, and tumor metastasis. Regulation of L-selectin at protein level has been...

  • 12-O-Tetradecanoylphorbol-13-Acetate Induces Up-Regulated Transcription of Variant 1 but Not Variant 2 of VIL2 in Esophageal Squamous Cell Carcinoma Cells via ERK1/2/AP-1/Sp1 Signaling. Zhang, Xiao-Dan; Xie, Jian-Jun; Liao, Lian-Di; Long, Lin; Xie, Yang-Min; Li, En-Min; Xu, Li-Yan // PLoS ONE;Apr2015, Vol. 10 Issue 4, p1 

    The membrane-cytoskeleton link organizer ezrin may be the most “dramatic” tumor marker, being strongly over-expressed in nearly one-third of human malignancies. However, the molecular mechanisms of aberrant ezrin expression still need to be clarified. Ezrin, encoded by the VIL2 gene,...

  • Transcriptional and post-translational regulation of mouse cation transport regulator homolog 1. Oh-hashi, Kentaro; Nomura, Yuki; Shimada, Kiyo; Koga, Hisashi; Hirata, Yoko; Kiuchi, Kazutoshi // Molecular & Cellular Biochemistry;Aug2013, Vol. 380 Issue 1/2, p97 

    Recently, cation transport regulator homolog 1 (Chac1) has been identified as a novel pro-apoptotic factor in cells under endoplasmic reticulum (ER) stress. Of the three major ER stress sensors, it is suggested that ATF4 participates in the transcriptional regulation of Chac1 gene expression....

  • Non-Equilibrium Hyperbolic Transport in Transcriptional Regulation. Hernández-Lemus, Enrique; Correa-Rodríguez, María D. // PLoS ONE;2011, Vol. 6 Issue 7, p1 

    In this work we studied memory and irreversible transport phenomena in a non-equilibrium thermodynamical model for genomic transcriptional regulation. Transcriptional regulation possess an extremely complex phenomenology, and it is, of course, of foremost importance in organismal cell...

  • Opinion: Coat proteins: shaping membrane transport. Bonifacino, Juan S.; Lippincott-Schwartz, Jennifer // Nature Reviews Molecular Cell Biology;May2003, Vol. 4 Issue 5, p409 

    Coat proteins allow the selective transfer of macromolecules from one membrane-enclosed compartment to another by concentrating macromolecules into specialized membrane patches and then deforming these patches into small coated vesicles. Recent findings indicate that coat proteins might also...

  • active transport:. Schlegel, Rolf H. J. // Encyclopedic Dictionary of Plant Breeding & Related Subjects;2003, p10 

    A definition of the term "Active Transport," which refers to the passage of substances across a cell membrane against a concentration gradient that requires energy, is presented.

  • Structural biology: Trimeric ion-channel design. Silberberg, Shai D.; Swartz, Kenton J. // Nature;7/30/2009, Vol. 460 Issue 7255, p580 

    The authors offer insights on research papers about the first X-ray crystal structure of a type of P2X receptor and the structure of another trimeric ion channel which reveals unanticipated similarities with P2X receptors. They explain that X-ray structure is a game-changer, providing crucial...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics