TITLE

Screening of transgenic proteins expressed in transgenic food crops for the presence of short amino acid sequences identical to potential, IgE -- binding linear epitopes of allergens

AUTHOR(S)
Kleter, Gijs A.; Peijnenburg, Ad A. C. M.
PUB. DATE
January 2002
SOURCE
BMC Structural Biology;2002, Vol. 2, p8
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Transgenic proteins expressed by genetically modified food crops are evaluated for their potential allergenic properties prior to marketing, among others by identification of short identical amino acid sequences that occur both in the transgenic protein and allergenic proteins. A strategy is proposed, in which the positive outcomes of the sequence comparison with a minimal length of six amino acids are further screened for the presence of potential linear IgE-epitopes. This double track approach involves the use of literature data on IgE-epitopes and an antigenicity prediction algorithm. Results: Thirty-three transgenic proteins have been screened for identities of at least six contiguous amino acids shared with allergenic proteins. Twenty-two transgenic proteins showed positive results of six- or seven-contiguous amino acids length. Only a limited number of identical stretches shared by transgenic proteins (papaya ringspot virus coat protein, acetolactate synthase GH50, and glyphosate oxidoreductase) and allergenic proteins could be identified as (part of) potential linear epitopes. Conclusion: Many transgenic proteins have identical stretches of six or seven amino acids in common with allergenic proteins. Most identical stretches are likely to be false positives. As shown in this study, identical stretches can be further screened for relevance by comparison with linear IgE-binding epitopes described in literature. In the absence of literature data on epitopes, antigenicity prediction by computer aids to select potential antibody binding sites that will need verification of IgE binding by sera binding tests. Finally, the positive outcomes of this approach warrant further clinical testing for potential allergenicity.
ACCESSION #
29323534

 

Related Articles

  • Identification and Characterization of Plant Allergens Using Proteomic Approaches. Law, Amanda; Gupta, Nitin; Louie, Melissa; Poddar, Richa; Ray, Animesh; Chakravarti, Deb N. // Current Proteomics;Jul2005, Vol. 2 Issue 2, p147 

    Rapid identification of protein allergens is important for understanding the mechanisms of pathogenesis of allergen induced diseases, and for improving diagnostic and therapeutic strategies. The most frequently used methods for characterizing protein allergens are two dimensional gel...

  • Pt2L4 Protein, a Homologue to Hev b 5 from Rubber Tree, May Not Be Responsible for the Cross-Reactions to Cassava Show by People Allergic to Latex. Batista de Souza, Cláudia Regina; Beezhold, Donald; Branco Carvalho, Luiz Joaquim C. // Protein & Peptide Letters;Sep2008, Vol. 15 Issue 9, p900 

    Pt2L4 is a protein from cassava homologue to Hevb5, a principal allergen from latex. Here we aimed to elucidate immunological relationships between these proteins. Our results revealed that epitopes found in Hev b 5 are not entirely conserved in Pt2L4 which is not recognized by IgE from patients...

  • Update of the List of Allergenic Proteins From Milk, Based on Local Amino Acid Sequence Identity with Known Epitopes From Bovine Milk Proteins -- a Short Report. Minkiewicz, Piotr; Dziuba, Jerzy; Gładkowska-Balewicz, Izabela // Polish Journal of Food & Nutrition Sciences;2011, Vol. 61 Issue 2, p153 

    The study involved the screening of protein sequence database nrdb 95 for sequences containing fragments identical with experimentally proven sequential epitopes of bovine milk proteins. Such fragments were found in proteins from milk of the buffalo (Bubalus bubalis), yak (Bos grunniens), goat...

  • Characterization of allergenic epitopes of Ory s1 protein from Oryza sativa and its homologs. Sharma, Ruchi; Singh, Ashok Kumar; Umashankar, Vetrivel // Bioinformation;2009, Vol. 4 Issue 1, p12 

    Vaccination is the most effective technique suggested now days for allergy treatment. Recombinant-based approaches are mostly focused on genetic modification of allergens to produce molecules with reduced allergenic activity and conserved antigenicity. The molecules developed for vaccination in...

  • An Allergen Portrait Gallery: Representative Structures and an Overview of IgE Binding Surfaces. Schein, Catherine H.; Ivanciuc, Ovidiu; Midoro-Horiuti, Terumi; Goldblum, Randall M.; Braun, Werner // Bioinformatics & Biology Insights;2010, Issue 4, p113 

    Recent progress in the biochemical classification and structural determination of allergens and allergen-antibody complexes has enhanced our understanding of the molecular determinants of allergenicity. Databases of allergens and their epitopes have facilitated the clustering of allergens...

  • NetCTLpan: pan-specific MHC class I pathway epitope predictions. Stranzl, Thomas; Larsen, Mette Voldby; Lundegaard, Claus; Nielsen, Morten // Immunogenetics;Jun2010, Vol. 62 Issue 6, p357 

    Reliable predictions of immunogenic peptides are essential in rational vaccine design and can minimize the experimental effort needed to identify epitopes. In this work, we describe a pan-specific major histocompatibility complex (MHC) class I epitope predictor, NetCTLpan. The method integrates...

  • Mycobacterium tuberculosis Rv0679c protein sequences involved in host-cell infection: Potential TB vaccine candidate antigen. Cifuentes, Diana P.; Ocampo, Marisol; Curtidor, Hernando; Vanegas, Magnolia; Forero, Martha; Patarroyo, Manuel E.; Patarroyo, Manuel A. // BMC Microbiology;2010, Vol. 10, p109 

    Background: To date, the function of many hypothetical membrane proteins of Mycobacterium tuberculosis is still unknown and their involvement in pathogen-host interactions has not been yet clearly defined. In this study, the biological activity of peptides derived from the hypothetical membrane...

  • New Tags for Recombinant Protein Detection and O-Glycosylation Reporters. Petris, Gianluca; Bestagno, Marco; Arnoldi, Francesca; Burrone, Oscar R. // PLoS ONE;May2014, Vol. 9 Issue 5, p1 

    Monoclonal antibodies (mAbs), because of their unique specificity, are irreplaceable tools for scientific research. Precise mapping of the antigenic determinants allows the development of epitope tagging approaches to be used with recombinant proteins for several purposes. Here we describe a new...

  • Prediction and mapping of IgE motif epitopes in proteins of Genetically Modified Foods for Immunotherapy strategy. Pallavi Somvanshi; Singh, Vijay; Seth, P. K. // Internet Journal of Infectious Diseases;2008, Vol. 6 Issue 2, p8 

    There is a constant need for assessment of potential allergenic and their patterns of cross-reactivity of genetically modified food (GMF) and pharmaceutical; in which novel proteins are introduced into the human food chain. The aspect for its identification is still in its nascent stage, which...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics