TITLE

Cross-species global and subset gene expression profiling identifies genes involved in prostate cancer response to selenium

AUTHOR(S)
Schlicht, Michael; Matysiak, Brian; Brodzeller, Tracy; Xinyu Wen; Hang Liu; Guohui Zhou; Dhir, Rajiv; Hessner, Martin J.; Tonellato, Peter; Suckow, Mark; Pollard, Morris; Datta, Milton W.
PUB. DATE
January 2004
SOURCE
BMC Genomics;2004, Vol. 5, p58
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Gene expression technologies have the ability to generate vast amounts of data, yet there often resides only limited resources for subsequent validation studies. This necessitates the ability to perform sorting and prioritization of the output data. Previously described methodologies have used functional pathways or transcriptional regulatory grouping to sort genes for further study. In this paper we demonstrate a comparative genomics based method to leverage data from animal models to prioritize genes for validation. This approach allows one to develop a disease-based focus for the prioritization of gene data, a process that is essential for systems that lack significant functional pathway data yet have defined animal models. This method is made possible through the use of highly controlled spotted cDNA slide production and the use of comparative bioinformatics databases without the use of cross-species slide hybridizations. Results: Using gene expression profiling we have demonstrated a similar whole transcriptome gene expression patterns in prostate cancer cells from human and rat prostate cancer cell lines both at baseline expression levels and after treatment with physiologic concentrations of the proposed chemopreventive agent Selenium. Using both the human PC3 and rat PAII prostate cancer cell lines have gone on to identify a subset of one hundred and fifty-four genes that demonstrate a similar level of differential expression to Selenium treatment in both species. Further analysis and data mining for two genes, the Insulin like Growth Factor Binding protein 3, and Retinoic X Receptor alpha, demonstrates an association with prostate cancer, functional pathway links, and protein-protein interactions that make these genes prime candidates for explaining the mechanism of Selenium's chemopreventive effect in prostate cancer. These genes are subsequently validated by western blots showing Selenium based induction and using tissue microarrays to demonstrate a significant association between downregulated protein expression and tumorigenesis, a process that is the reverse of what is seen in the presence of Selenium. Conclusions: Thus the outlined process demonstrates similar baseline and selenium induced gene expression profiles between rat and human prostate cancers, and provides a method for identifying testable functional pathways for the action of Selenium's chemopreventive properties in prostate cancer.
ACCESSION #
28859348

 

Related Articles

  • PTK6 Regulates IGF-1-Induced Anchorage-Independent Survival. Irie, Hanna Y.; Shrestha, Yashaswi; Selfors, Laura M.; Frye, Fabianne; Iida, Naoko; Zhigang Wang; Lihua Zou; Jun Yao; Yiling Lu; Epstein, Charles B.; Natesan, Sridaran; Richardson, Andrea L.; Polyak, Kornelia; Mills, Gordon B.; Hahn, William C.; Brugge, Joan S. // PLoS ONE;2010, Vol. 5 Issue 7, p1 

    Background: Proteins that are required for anchorage-independent survival of tumor cells represent attractive targets for therapeutic intervention since this property is believed to be critical for survival of tumor cells displaced from their natural niches. Anchorage-independent survival is...

  • Methylation of a CTCF-dependent boundary controls imprinted expression of the Igf2 gene. Bell, Adam C.; Felsenfeld, Gary // Nature;5/25/2000, Vol. 405 Issue 6785, p482 

    Discusses the expression of the insulin-like growth factor 2 (Igf2) and H19 genes. Differences in the expression of the respective allele, despite the fact that the two share an enhancer; Results of deletion from this region and the loss of the imprinting of both H19 and Igf2; Various methods...

  • IGF-1R inhibition enhances radiosensitivity and delays double-strand break repair by both non-homologous end-joining and homologous recombination. Chitnis, M M; Lodhia, K A; Aleksic, T; Gao, S; Protheroe, A S; Macaulay, V M // Oncogene;11/6/2014, Vol. 33 Issue 45, p5262 

    Inhibition of type 1 insulin-like growth factor receptor (IGF-1R) enhances tumor cell sensitivity to ionizing radiation. It is not clear how this effect is mediated, nor whether this approach can be applied effectively in the clinic. We previously showed that IGF-1R depletion delays repair of...

  • Effect of lycopene on insulin-like growth factor-I, IGF binding protein-3 and IGF type-I receptor in prostate cancer cells. Kanagaraj, P.; Vijayababu, M. R.; Ravisankar, B.; Anbalagan, J.; Aruldhas, M. M.; Arunakaran, J. // Journal of Cancer Research & Clinical Oncology;Jun2007, Vol. 133 Issue 6, p351 

    Prostate cancer is the second most common cancer that leads to death in elderly men. The risk of prostate cancer prevalence is often associated with the elevated level of insulin-like growth factor-I (IGF-I) and decreased level of IGF-binding protein 3 (IGFBP-3). Lycopene, a carotenoid, reduces...

  • Activation of Various Downstream Signaling Molecules by IGFBP-3. Mohammad Shahjee, Hanief; Bhattacharyya, Nisan // Journal of Cancer Therapy;Aug2014, Vol. 5 Issue 9, p830 

    Insulin-like growth factor binding protein-3 (IGFBP-3), a secretory protein, is the most abundant IGF binding protein present in human serum among all IGF binding proteins. IGFBP-3 shows decreased level of expression in cancerous cells but has been known to be present in significant amounts in...

  • Contribution of the orphan nuclear receptor Nur77 to the apoptotic action of IGFBP-3. Kuk-Wha Lee; Cobb, Laura J.; Paharkova-Vatchkova, Vladislava; Liu, Bingrong; Milbrandt, Jeffrey; Cohen, Pinchas // Carcinogenesis;Aug2007, Vol. 28 Issue 8, p1653 

    Tumor suppression by insulin-like growth factor-binding protein-3 (IGFBP-3) has been demonstrated to occur via insulin-like growth factor-dependent and -independent mechanisms in vitro and in vivo. We have recently described IGFBP-3-induced mitochondrial translocation of the nuclear receptors...

  • Duplication of the IGFBP-2 Gene in Teleost Fish: Protein Structure and Functionality Conservation and Gene Expression Divergence. Zhou, Jianfeng; Wenhong Li; Kamei, Hiroyasu; Duan, Cunming // PLoS ONE;2008, Vol. 3 Issue 12, p1 

    Background: Insulin-like growth factor binding protein-2 (IGFBP-2) is a secreted protein that binds and regulates IGF actions in controlling growth, development, reproduction, and aging. Elevated expression of IGFBP-2 is often associated with progression of many types of cancers....

  • MiRNA Expression in Psoriatic Skin: Reciprocal Regulation of hsa-miR-99a and IGF-1R. Lerman, Galya; Avivi, Camila; Mardoukh, Corine; Barzilai, Aviv; Tessone, Ariel; Gradus, Ben; Pavlotsky, Felix; Barshack, Iris; Polak-Charcon, Sylvie; Orenstein, Arie; Hornstein, Eran; Sidi, Yechezkel; Avni, Dror // PLoS ONE;2011, Vol. 6 Issue 6, p1 

    Background: Psoriasis is a complex disease at the cellular, genomic and genetic levels. The role of microRNAs in skin development was shown in a keratinocyte-specific Dicer knockout mouse model. Considering that two main characteristics of psoriasis are keratinocytes hyperproliferation and...

  • IGFBP-rP1 may be Involved in Decidualization of ESCs.  // Fertility Weekly;12/17/2007, p10 

    The article reports on the association of insulin-like growth factor-binding protein-related protein 1 (IGFBP-rP1) with decidualization of endometrial stromal cells (ESCs), based on a study in Japan. Enhanced IGFBP-rP1 and prolactin messenger RNA expression is upregulated in the mid-to-late...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics