Microarray genomotyping of key experimental strains of Neisseria gonorrhoeae reveals gene complement diversity and five new neisserial genes associated with Minimal Mobile Elements

Snyder, Lori A. S.; Davies, John K.; Saunders, Nigel J.
January 2004
BMC Genomics;2004, Vol. 5, p1
Academic Journal
Background: There are four widely used experimental strains of N. gonorrhoeae, one of which has been sequenced and used as the basis for the construction of a multi-strain, mutli-species panneisserial microarray. Although the N. gonorrhoeae population structure is thought to be less diverse than N. meningitidis, there are some recognized gene-complement differences between strains, including the 59 genes of the Gonococcal Genetic Island. In this study we have investigated the three experimental strains that have not been sequenced to determine the extent and nature of their similarities and differences. Results: Using the Pan-Neisseria microarray, three commonly used gonococcal laboratory experimental strains were investigated (F62, MS11, & FA19). Genes absent from these strains, but present in strain FA1090, were assessed as is possible with typical microarrays. Due to the design of this microarray, additional genes were also identified. Differences were associated with Minimal Mobile Elements (MMEs) or known divergences. Genomotyping indicates the presence of genes previously only described in meningococci and shows the presence of the complete Gonococcal Genetic Island in N. gonorrhoeae strain FA19. Five new neisserial genes were identified through microarray genomotyping and subsequent sequencing of two divergent MMEs in N. gonorrhoeae strain MS11 and four MMEs in N. gonorrhoeae strain FA19. No differences were identified between N. gonorrhoeae strains FA1090 and F62, indicating that these strains are very similar. Conclusion: This study shows extensive similarity between the experimental strains, associated with a varying number of strain-specific genes. This provides a framework for those working with these strains to refer to the available gonococcal genome sequence, and is the first detailed comparison of gene complements between gonococcal strains.


Related Articles

  • Comparative analysis of two Neisseria gonorrhoeae genome sequences reveals evidence of mobilization of Correia Repeat Enclosed Elements and their role in regulation. Snyder, Lori A. S.; Cole, Jeff A.; Pallen, Mark J. // BMC Genomics;2009, Vol. 10, Special section p1 

    Background: The Correia Repeat Enclosed Element (CREE) of the Neisseria spp., with its inverted repeat and conserved core structure, can generate a promoter sequence at either or both ends, can bind IHF, and can bind RNase III and either be cleaved by it or protected by it. As such, the presence...

  • Neisseria meningitidis and Neisseria gonorrhoeae Bacteremia Associated with C6, C7, or C8 Deficiency. Petersen, Bruce H.; Lee, Terrence J.; Snyderman, Ralph; Brooks, Geo F. // Annals of Internal Medicine;Jun79, Vol. 90 Issue 6, p917 

    Focuses on the diagnosed cases of homozygous deficiency of the sixth, seventh or eighth components of complement. Role of complement in the inflammatory response and in the immunologically meditated host defense; Data from patients with deficiency of sixth component of complement; Details on...

  • Clonal Expansion of Sequence Type (ST-)5 and Emergence of ST-7 in Serogroup A Meningococci, Africa. Nicolas, Pierre; Decousset, Laurent; Riglet, Vincent; Castelli, Philippe; Stor, Richard; Blanchet, Guy // Emerging Infectious Diseases;Sep/Oct2001, Vol. 7 Issue 5, p849 

    One hundred four serogroup A meningococci in our collection, isolated in Africa from 1988 to 1999, were characterized by multilocus sequence typing (MLST). Our results and data from the Internet indicate that sequence type 5 (ST-5) strains were responsible for most of African outbreaks and...

  • Previously unrecognized vaccine candidates against group B meningococcus identified by DNA microarrays. Grifantini, Renata; Bartolini, Erika; Muzzi, Alessandro; Draghi, Monia; Frigimelica, Elisabetta; Berger, Joel; Ratti, Giulio; Petracca, Roberto; Galli, Giuliano; Agnusdei, Mauro; Monica Giuliani, Marzia; Santini, Laura; Brunelli, Brunella; Tettelin, HervĂ©; Rappuoli, Rino; Randazzo, Filippo; Grandi, Guido // Nature Biotechnology;Sep2002, Vol. 20 Issue 9, p914 

    We have used DNA microarrays to follow Neisseria meningitidis serogroup B (MenB) gene regulation during interaction with human epithelial cells. Host-cell contact induced changes in the expression of 347 genes, more than 30% of which encode proteins with unknown function. The upregulated genes...

  • Heterologous oligonucleotide microarrays for transcriptomics in a non-model species; a proof-of-concept study of drought stress in Musa. Davey, Mark W.; Graham, Neil S.; Vanholme, Bartel; Swennen, Rony; May, Sean T.; Keulemans, Johan // BMC Genomics;2009, Vol. 10, p436 

    Background: 'Systems-wide' approaches such as microarray RNA-profiling are ideally suited to the study of the complex overlapping responses of plants to biotic and abiotic stresses. However, commercial microarrays are only available for a limited number of plant species and development costs are...

  • Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491. Parkhill, J.; Achtman, M. // Nature;3/30/2000, Vol. 404 Issue 6777, p502 

    Studies the complete genome sequence of a serogroup A strain of Neisseria meningitidis , z2491. G+C content of the genome; Unique characteristics of the Z2491; Natural competence of neisserial species; Repeat-mediated gene rearrangements at other sites; General operation of three mechanisms of...

  • Pathogenic neisseriae: surface modulation, pathogenesis and infection control. Virji, Mumtaz // Nature Reviews Microbiology;Apr2009, Vol. 7 Issue 4, p274 

    Although renowned as a lethal pathogen, Neisseria meningitidis has adapted to be a commensal of the human nasopharynx. It shares extensive genetic and antigenic similarities with the urogenital pathogen Neisseria gonorrhoeae but displays a distinct lifestyle and niche preference. Together, they...

  • Species status of Neisseria gonorrhoeae: evolutionary and epidemiological inferences from multilocus sequence typing. Bennett, Julia S.; Jolley, Keith A.; Sparling, P. Frederick; Saunders, Nigel J.; Hart, C. Anthony; Feavers, Ian M.; Maiden, Martin C. J. // BMC Biology;2007, Vol. 5, p1 

    Background: Various typing methods have been developed for Neisseria gonorrhoeae, but none provide the combination of discrimination, reproducibility, portability, and genetic inference that allows the analysis of all aspects of the epidemiology of this pathogen from a single data set....

  • The Long and Winding Road (Apologies to the Beatles). Seifert, Hank // PLoS Pathogens;8/27/2015, Vol. 11 Issue 8, p1 

    The author presents insights on the pathogens Neisseria (N.) gonorrheae and N. meningitidis. It notes that the pathogens are closely related organisms that colonize distinct physiologic sites within people and cause very different diseases. It cites that the pathogens most often colonize people...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics