The 10 sea urchin receptor for egg jelly proteins (SpREJ) are members of the polycystic kidney disease-1 (PKD1) family

Gunaratne, H Jayantha; Moy, Gary W; Kinukawa, Masashi; Miyata, Shinji; Mah, Silvia A; Vacquier, Victor D
January 2007
BMC Genomics;2007, Vol. 8, p235
Academic Journal
Background: Mutations in the human polycystic kidney disease-1 (hPKD1) gene result in ~85% of cases of autosomal dominant polycystic kidney disease, the most frequent human monogenic disease. PKD1 proteins are large multidomain proteins involved in a variety of signal transduction mechanisms. Obtaining more information about members of the PKD1 family will help to clarify their functions. Humans have five hPKD1 proteins, whereas sea urchins have 10. The PKD1 proteins of the sea urchin, Strongylocentrotus purpuratus, are referred to as the Receptor for Egg Jelly, or SpREJ proteins. The SpREJ proteins form a subfamily within the PKD1 family. They frequently contain C-type lectin domains, PKD repeats, a REJ domain, a GPS domain, a PLAT/LH2 domain, 1-11 transmembrane segments and a C-terminal coiled-coil domain. Results: The 10 full-length SpREJ cDNA sequences were determined. The secondary structures of their deduced proteins were predicted and compared to the five human hPKD1 proteins. The genomic structures of the 10 SpREJs show low similarity to each other. All 10 SpREJs are transcribed in either embryos or adult tissues. SpREJs show distinct patterns of expression during embryogenesis. Adult tissues show tissue-specific patterns of SpREJ expression. Conclusion: Possession of a REJ domain of about 600 residues defines this family. Except for SpREJ1 and 3, that are thought to be associated with the sperm acrosome reaction, the functions of the other SpREJ proteins remain unknown. The sea urchin genome is one-fourth the size of the human genome, but sea urchins have 10 SpREJ proteins, whereas humans have five. Determination of the tissue specific function of each of these proteins will be of interest to those studying echinoderm development. Sea urchins are basal deuterostomes, the line of evolution leading to the vertebrates. The study of individual PKD1 proteins will increase our knowledge of the importance of this gene family.


Related Articles

  • Gene Regulatory Network Interactions in Sea Urchin Endomesoderm Induction. Sethi, Aditya J.; Angerer, Robert C.; Angerer, Lynne M. // PLoS Biology;Feb2009, Vol. 7 Issue 2, pe1000029 

    A classic embryonic induction in sea urchins is described in terms of interacting gene regulatory networks and a signal transduction system that connects them.

  • Trp2 regulates entry of Ca2+ into mouse sperm triggered by egg ZP3. Jungnickel, Melissa K.; Marrero, Hector; Birnbaumer, Lutz; Lémos, José R.; Florman, Harvey M. // Nature Cell Biology;May2001, Vol. 3 Issue 5, p499 

    In many cells, receptor activation initiates sustained Ca[SUP2+] entry which is critical in signal transduction[SUP1]. Mammalian transient receptor potential (Trp) proteins, which are homologous to the Drosophila photoreceptor-cell Trp protein, have emerged as candidate subunits of the ion...

  • Innate immunity: Living without Toll-like receptors. Bell, Jennifer // Nature Reviews Immunology;Apr2003, Vol. 3 Issue 4, p261 

    Discusses the results of a study, which demonstrated that in humans, the main Toll-like receptor (TLR) signal-transduction pathway is not essential for survival. Information on the TLR signal; Classes of microorganisms, which are characterized by molecular patterns; Examples of extracellular...

  • How G proteins turn off. Goody, Roger S. // Nature;11/17/1994, Vol. 372 Issue 6503, p220 

    Reports on the importance of G proteins in transduction of signals that are received by cell-surface receptors. Structure of the alpha-subunits of two G proteins; Stable complexes from G proteins and guanosine diphosphate in the presence of fluoride ions; Guanosine triphosphate hydrolysis;...

  • Platelet receptor signalling. Clemetson, Kenneth J.; Clemetson, Jeannine M. // Hematology Journal;2004 Supplement 3, Vol. 5, pS159 

    Studies platelet receptor signaling. Receptor categories; Mechanisms of signaling; Crosstalk between receptors and their pathways.

  • New insights into pre-BCR and BCR signalling with relevance to B cell malignancies. Rickert, Robert C. // Nature Reviews Immunology;Sep2013, Vol. 13 Issue 9, p701 

    A correction to the article "New Insights Into Pre-BCR and BCR Signalling With Relevance to B Cell Malignancies" that was published in a 2013 issue is presented.

  • Genetic Interactions of STAT3 and Anticancer Drug Development. Bingliang Fang // Cancers;Mar2014, Vol. 6 Issue 1, p494 

    Signal transducer and activator of transcription 3 (STAT3) plays critical roles in tumorigenesis and malignant evolution and has been intensively studied as a therapeutic target for cancer. A number of STAT3 inhibitors have been evaluated for their antitumor activity in vitro and in vivo in...

  • Tissue Regeneration and Biomineralization in Sea Urchins: Role of Notch Signaling and Presence of Stem Cell Markers. Reinardy, Helena C.; Emerson, Chloe E.; Manley, Jason M.; Bodnar, Andrea G. // PLoS ONE;8/12/2015, Vol. 10 Issue 8, p1 

    Echinoderms represent a phylum with exceptional regenerative capabilities that can reconstruct both external appendages and internal organs. Mechanistic understanding of the cellular pathways involved in regeneration in these animals has been hampered by the limited genomic tools and limited...

  • Discrete Dynamics Model for the Speract-Activated Ca2+ Signaling Network Relevant to Sperm Motility. Espinal, Jesús; Aldana, Maximino; Guerrero, Adán; Wood, Christopher; Darszon, Alberto; Martínez-Mekler, Gustavo // PLoS ONE;2011, Vol. 6 Issue 8, p1 

    Understanding how spermatozoa approach the egg is a central biological issue. Recently a considerable amount of experimental evidence has accumulated on the relation between oscillations in intracellular calcium ion concentration ([Ca2z]i) in the sea urchin sperm flagellum, triggered by peptides...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics