TITLE

Sensitive and robust gene expression changes in fish exposed to estrogen -- a microarray approach

AUTHOR(S)
Gunnarsson, Lina; Kristiansson, Erik; Förlin, Lars; Nerman, Olle; Larsson, D G Joakim
PUB. DATE
January 2007
SOURCE
BMC Genomics;2007, Vol. 8, p149
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Vitellogenin is a well established biomarker for estrogenic exposure in fish. However, effects on gonadal differentiation at concentrations of estrogen not sufficient to give rise to a measurable vitellogenin response suggest that more sensitive biomarkers would be useful. Induction of zona pellucida genes may be more sensitive but their specificities are not as clear. The objective of this study was to find additional sensitive and robust candidate biomarkers of estrogenic exposure. Results: Hepatic mRNA expression profiles were characterized in juvenile rainbow trout exposed to a measured concentration of 0.87 and 10 ng ethinylestradiol/L using a salmonid cDNA microarray. The higher concentration was used to guide the subsequent identification of generally more subtle responses at the low concentration not sufficient to induce vitellogenin. A metaanalysis was performed with data from the present study and three similar microarray studies using different fish species and platforms. Within the generated list of presumably robust responses, several well-known estrogen-regulated genes were identified. Two genes, confirmed by quantitative RT-PCR (qPCR), fulfilled both the criteria of high sensitivity and robustness; the induction of the genes encoding zona pellucida protein 3 and a nucleoside diphosphate kinase (nm23). Conclusion: The cross-species, cross-platform meta-analysis correctly identified several robust responses. This adds confidence to our approach used for identifying candidate biomarkers. Specifically, we propose that analyses of an nm23 gene together with zona pellucida genes may increase the possibilities to detect an exposure to low levels of estrogenic compounds in fish.
ACCESSION #
28858832

 

Related Articles

  • Zona Pellucida Domain-Containing Protein &bgr;-Tectorin is Crucial for Zebrafish Proper Inner Ear Development. Chung-Hsiang Yang; Chia-Hsiung Cheng; Gen-Der Chen; Wei-Hao Liao; Yi-Chung Chen; Kai-Yun Huang; Hwang, Pung-Pung; Hwang, Sheng-Ping L.; Huang, Chang-Jen // PLoS ONE;2011, Vol. 6 Issue 8, p1 

    Background: The zona pellucida (ZP) domain is part of many extracellular proteins with diverse functions from structural components to receptors. The mammalian b-tectorin is a protein of 336 amino acid residues containing a single ZP domain and a putative signal peptide at the N-terminus of the...

  • Aromatase and estrogen receptor alpha mRNA expression as prognostic biomarkers in patients with astrocytomas. Dueñas Jiménez, J.; Candanedo Arellano, A.; Santerre, A.; Orozco Suárez, S.; Sandoval Sánchez, H.; Feria Romero, I.; López-Elizalde, R.; Alonso Venegas, M.; Netel, B.; Torre Valdovinos, B.; Dueñas Jiménez, S. // Journal of Neuro-Oncology;Sep2014, Vol. 119 Issue 2, p275 

    Estrogens are oncogenic hormones at a high level in breast, prostate, endometrial and lung cancer. Estrogens are synthesized by aromatase which has been used as a biomarker both in breast and lung cancer. Estrogen biological activities are executed by their classic receptors (ERα and ERβ)....

  • Estrogen receptor β promoter methylation: a potential indicator of malignant changes in breast cancer. Lei Gao; Xiaolong Qi; Kaiwen Hu; Ruili Zhu; Wei Xu; Shipeng Sun; Lixin Zhang; Ximing Yang; Baojin Hua; Guijian Liu; Gao, Lei; Qi, Xiaolong; Hu, Kaiwen; Zhu, Ruili; Xu, Wei; Sun, Shipeng; Zhang, Lixin; Yang, Ximing; Hua, Baojin; Liu, Guijian // Archives of Medical Science;Feb2016, Vol. 12 Issue 1, p129 

    Introduction: Estrogen receptor β (ERβ) always lacks expression in estrogen-dependent tumors, which may result from gene inactivation by methylation. In this study, we aimed to determine whether aberrant methylation of the ERβ promoter is associated with decreased ERβ...

  • Computational Analysis of mRNA Expression Profiles Identifies MicroRNA-29a/c as Predictor of Colorectal Cancer Early Recurrence. Tai-Yue Kuo; Edward Hsi; I-Ping Yang; Pei-Chien Tsai; Jaw-Yuan Wang; Suh-Hang Hank Juo // PLoS ONE;Feb2012, Vol. 7 Issue 2, p1 

    Colorectal cancer (CRC) is one of the leading malignant cancers with a rapid increase in incidence and mortality. The recurrences of CRC after curative resection are sometimes unavoidable and often take place within the first year after surgery. MicroRNAs may serve as biomarkers to predict early...

  • White Matter Correlates of Neuropsychological Dysfunction in Systemic Lupus Erythematosus. Jung, Rex E.; Chavez, Robert S.; Flores, Ranee A.; Qualls, Clifford; Sibbitt,Jr., Wilmer L.; Roldan, Carlos A. // PLoS ONE;Jan2012, Vol. 7 Issue 1, p1 

    Patients diagnosed with Systemic Lupus Erythematosus have similar levels of neuropsychological dysfunction (i.e., 20-50%) as those with Neuropsychiatric Systemic Lupus Erythematosus (NPSLE). We hypothesized a gradient between cognition and white matter integrity, such that strongest...

  • Characteristics of Hepatic IGF-II Expression and Monitored Levels of Circulating IGF-II mRNA in Metastasis of Hepatocellular Carcinoma. Jing Qian; Dengfu Yao; Zhizhen Dong; Wei Wu; Liwei Qiu; Ninghua Yao; Shanshan Li; Yinzhu Bian; Zhiwei Wang; Gongsheng Shi // American Journal of Clinical Pathology;Nov2010, Vol. 134 Issue 5, p799 

    The prognosis of hepatocellular carcinoma (HCC) remains dismal. Insulin-like growth factor II (IGF-II), a fetal growth factor, is highly expressed during HCC development. We examined serum IGF-II levels and circulating IGF-II messenger RNA (mRNA) expression and analyzed the clinicopathologic...

  • DDAH2 mRNA expression is inversely associated with some cardiovascular risk-related features in healthy young adults. Puchau, Blanca; Hermsdorff, Helen Hermana M.; Zulet, M. Ángeles; Martínez, J. Alfredo // Disease Markers;2009, Vol. 27 Issue 1, p37 

    The purpose of this study was to evaluate whether the mRNA expression profiles of three genes (PRMT1, DDAH2 and NOS3) are related to ADMA metabolism and signalling, and the potential relationships with anthropometrical, biochemical, lifestyle and inflammatory indicators in healthy young adults....

  • Pre-profiling factors influencing serum microRNA levels. MacLellan, Sara A.; MacAulay, Calum; Lam, Stephen; Garnis, Cathie // BMC Clinical Pathology;2014, Vol. 14 Issue 1, p1 

    Background MicroRNAs (miRNAs) are non-coding RNAs that negatively regulate gene expression by preventing the translation of specific mRNA transcripts. Recent studies have shown that miRNAs are stably expressed in human serum samples, making them good candidates for the non-invasive detection of...

  • Differential expression of p42.3 in low- and high-grade gliomas. Weiqing Wan; Xiaoqing Xu; Guijun Jia; Wenmei Li; Junmei Wang; Tong Ren; Zhen Wu; Junting Zhang; Liwei Zhang; Youyong Lu // World Journal of Surgical Oncology;2014, Vol. 12 Issue 1, p1 

    Background Malignant gliomas are the most common form of primary malignant brain tumor. It has recently been suggested that genetic changes are involved in the progression of malignant gliomas. In previous studies, a novel gene, p42.3, was characterized as a tumor-specific gene that encodes a...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics