TITLE

STATc is a key regulator of the transcriptional response to hyperosmotic shock

AUTHOR(S)
Jianbo Na; Tunggal, Budi; Eichinger, Ludwig
PUB. DATE
January 2007
SOURCE
BMC Genomics;2007, Vol. 8, p123
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Dictyostelium discoideum is frequently subjected to environmental changes in its natural habitat, the forest soil. In order to survive, the organism had to develop effective mechanisms to sense and respond to such changes. When cells are faced with a hypertonic environment a complex response is triggered. It starts with signal sensing and transduction and leads to changes in cell shape, the cytoskeleton, transport processes, metabolism and gene expression. Certain aspects of the Dictyostelium osmotic stress response have been elucidated, however, no comprehensive picture was available up to now. Results: To better understand the D. discoideum response to hyperosmotic conditions, we performed gene expression profiling using DNA microarrays. The transcriptional profile of cells treated with 200 mM sorbitol during a 2-hour time course revealed a time-dependent induction or repression of 809 genes, more than 15% of the genes on the array, which peaked 45 to 60 minutes after the hyperosmotic shock. The differentially regulated genes were applied to cluster analysis and functional annotation using gene GO terms. Two main responses appear to be the downregulation of the metabolic machinery and the up-regulation of the stress response system, including STATc. Further analysis of STATc revealed that it is a key regulator of the transcriptional response to hyperosmotic shock. Approximately 20% of the differentially regulated genes were dependent on the presence of STATc. Conclusion: At least two signalling pathways are activated in Dictyostelium cells subjected to hypertonicity. STATc is responsible for the transcriptional changes of one of them.
ACCESSION #
28858820

 

Related Articles

  • Control of Cyclin C Levels during Development of Dictyostelium. Greene, David M.; Duen-Wei Hsu; Pears, Catherine J. // PLoS ONE;2010, Vol. 5 Issue 5, p1 

    Background: Cdk8 and its partner cyclin C form part of the mediator complex which links the basal transcription machinery to regulatory proteins. The pair are required for correct regulation of a subset of genes and have been implicated in control of development in a number of organisms...

  • Identifying cis-acting DNA elements within the control region of glycogen phosphorylase 2 by DNaseI footprinting in Dictyostelium discoideum. Çöl, Bekir; Rutherford, Charles L. // Journal of Cell & Molecular Biology;2007, Vol. 6 Issue 2, p109 

    Glycogen phosphorylase 2 (encoded by gp2) is a key enzyme expressed during the development of Dictyostelium discoideum. The Gp2 enzyme breaks down glycogen into glucose monomers that are subsequently used to synthesize the terminal end products of cellular differentiation. This gene is an ideal...

  • Systematic evaluation of buffer influences on the development of Dictyostelium discoideum. Márquez López, Johanna; Sulzmann, Anja; Thewes, Sascha // Development Genes & Evolution;Jan2016, Vol. 226 Issue 1, p27 

    Development and cell differentiation are key features of the social amoeba Dictyostelium discoideum. Already at early developmental stages, the gene expression profile changes in the amoebae to make the cells aggregation competent. In the laboratory, development starts when the cells are washed...

  • PTEN Redundancy: Overexpressing lpten, a Homolog of Dictyostelium discoideum ptenA, the Ortholog of Human PTEN, Rescues All Behavioral Defects of the Mutant ptenA−. Lusche, Daniel F.; Wessels, Deborah; Richardson, Nicole A.; Russell, Kanoe B.; Hanson, Brett M.; Soll, Benjamin A.; Lin, Benjamin H.; Soll, David R. // PLoS ONE;Sep2014, Vol. 9 Issue 9, p1 

    Mutations in the tumor suppressor gene PTEN are associated with a significant proportion of human cancers. Because the human genome also contains several homologs of PTEN, we considered the hypothesis that if a homolog, functionally redundant with PTEN, can be overexpressed, it may rescue the...

  • Evolutionary biology: Farming writ small. Boomsma, Jacobus J. // Nature;1/20/2011, Vol. 469 Issue 7330, p308 

    The article presents a study which examines the bacterial husbandry practised by Dictyostelium slime moulds. The study reveals that an important fraction of Dictyostelium discoideum spores convey bacteria to inoculate new habitat with food, but the husbandry has stayed clone-specific. The...

  • Glycogen synthase kinase-3 enhances nuclear export of a Dictyostelium STAT protein. Singer, Rebecca S.; Dalton, Emma C.; Ryves, W. Jonathan; Masashi Fukuzawa; Williams, Jeffrey G.; Harwood, Adrian J. // EMBO Journal;10/15/2000, Vol. 19 Issue 20, p5483 

    Extracellular cAMP stimulates the rapid tyrosine phosphorylation and nuclear translocation of the Dictyostelium STAT protein Dd-STATa. Here we show that it also induces serine phosphorylation by GskA, a homologue of glycogen synthase kinase-3 (GSK-3). Tyrosine phosphorylation occurs within 10 s...

  • Rearrangement of cortex proteins constitutes an osmoprotective mechanism in Dictyostelium. Zischka, Hans; Oehme, Felix; Pintsch, Tanja; Ott, Alexander; Keller, Heike; Kellermann, Joseph; Schuster, Stephan C. // EMBO Journal;8/1/99, Vol. 18 Issue 15, p4241 

    Dictyostelium responds to hyperosmotic stress of 400 mOsm by a rapid reduction of its cell volume to 50%. The reduced cell volume is maintained as long as these osmotic conditions prevail. Dictyostelium does not accumulate compatible osmolytes to counteract the osmotic pressure applied. Using...

  • A functional connection of Dictyostelium paracaspase with the contractile vacuole and a possible partner of the vacuolar proton ATPase. Saheb, Entsar; Biton, Ithay; Maringer, Katherine; Bush, John // Journal of Biosciences;Sep2013, Vol. 38 Issue 3, p509 

    Dictyostelium discoideum possesses only one caspase family member, paracaspase ( pcp). Two separate mutant cell lines were first analysed: one cell line was an over-expressed GFP-tagged Pcp (GFP-Pcp), while the other cell line was a pcp-null ( pcp-). Microscopic analysis of cells expressing...

  • Silent information regulator 2 proteins encoded by Cryptosporidium parasites. Yasukawa, Hiro; Yagita, Kenji // Parasitology Research;Aug2010, Vol. 107 Issue 3, p707 

    Screening in a database has revealed that Cryptosporidium hominis encodes a silent information regulator 2 (Sir2), a nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylase. Cellular localization of the protein, ChSir2, was analyzed by the use of the social amoeba Dictyostelium...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics