TITLE

Derivation of species-specific hybridization-like knowledge out of cross-species hybridization results

AUTHOR(S)
Bar-Or, Carmiya; Bar-Eyal, Meira; Gal, Tali Z; Kapulnik, Yoram; Czosnek, Henryk; Koltai, Hinanit
PUB. DATE
January 2006
SOURCE
BMC Genomics;2006, Vol. 7, p110
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: One of the approaches for conducting genomics research in organisms without extant microarray platforms is to profile their expression patterns by using Cross-Species Hybridization (CSH). Several different studies using spotted microarray and CSH produced contradicting conclusions in the ability of CSH to reflect biological processes described by speciesspecific hybridization (SSH). Results: We used a tomato-spotted cDNA microarray to examine the ability of CSH to reflect SSH data. Potato RNA was hybridized to spotted cDNA tomato and potato microarrays to generate CSH and SSH data, respectively. Difficulties arose in obtaining transcriptomic data from CSH that reflected those obtained from SSH. Nevertheless, once the data was filtered for those corresponding to matching probe sets, by restricting proper cutoffs of probe homology, the CSH transcriptome data showed improved reflection of those of the SSH. Conclusions: This study evaluated the relative performance of CSH compared to SSH, and proposes methods to ensure that CSH closely reflects the biological process analyzed by SSH.
ACCESSION #
28858650

 

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