TITLE

EST2Prot: Mapping EST sequences to proteins

AUTHOR(S)
Shafer, Paul; Lin, David M; Yona, Golan
PUB. DATE
January 2006
SOURCE
BMC Genomics;2006, Vol. 7, p41
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: EST libraries are used in various biological studies, from microarray experiments to proteomic and genetic screens. These libraries usually contain many uncharacterized ESTs that are typically ignored since they cannot be mapped to known genes. Consequently, new discoveries are possibly overlooked. Results: We describe a system (EST2Prot) that uses multiple elements to map EST sequences to their corresponding protein products. EST2Prot uses UniGene clusters, substring analysis, information about protein coding regions in existing DNA sequences and protein database searches to detect protein products related to a query EST sequence. Gene Ontology terms, Swiss- Prot keywords, and protein similarity data are used to map the ESTs to functional descriptors. Conclusion: EST2Prot extends and significantly enriches the popular UniGene mapping by utilizing multiple relations between known biological entities. It produces a mapping between ESTs and proteins in real-time through a simple web-interface. The system is part of the Biozon database and is accessible at http://biozon.org/tools/est/.
ACCESSION #
28858609

 

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics