TITLE

Evaluation of low density array technology for quantitative parallel measurement of multiple genes in human tissue

AUTHOR(S)
Goulter, Andrew B; Harmer, Daniel W; Clark, Kenneth L
PUB. DATE
January 2006
SOURCE
BMC Genomics;2006, Vol. 7, p34
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Low density arrays (LDAs) have recently been introduced as a novel approach to gene expression profiling. Based on real time quantitative RT-PCR (QRT-PCR), these arrays enable a more focused and sensitive approach to the study of gene expression than gene chips, while offering higher throughput than more established approaches to QRT-PCR. We have now evaluated LDAs as a means of determining the expression of multiple genes simultaneously in human tissues and cells. Results: Comparisons between LDAs reveal low variability, with correlation coefficients close to 1. By performing 2-fold and 10-fold serial dilutions of cDNA samples in the LDAs we determined a clear linear relationship between the gene expression data points over 5 orders of magnitude. We also showed that it is possible to use LDAs to accurately and quantitatively detect 2-fold changes in target copy number as well as measuring genes that are expressed with low and high copy numbers in the range of 1 x 102 — 1 x 106 copies. Furthermore, the data generated by the LDA from a cell based pharmacological study were comparable to data generated by conventional QRT-PCR. Conclusion: LDAs represent a valuable new approach for sensitive and quantitative gene expression profiling.
ACCESSION #
28858607

 

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