TITLE

Comparative analysis of expression of histone H2a genes in mouse

AUTHOR(S)
Hiromi Nishida; Takahiro Suzuki; Hiroki Ookawa; Yasuhiro Tomaru; Yoshihide Hayashizaki
PUB. DATE
January 2005
SOURCE
BMC Genomics;2005, Vol. 6, p108
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: At least 18 replication-dependent histone H2a genes are distributed in 3 Hist gene clusters on different chromosomes of the mouse genome. In this analysis we designed specific PCR primers for each histone H2a transcript and studied the expression levels and patterns using quantitative RT-PCR (qRT-PCR). In addition, we compared histone H3 K9 acetylation levels in the promoter regions of H2a genes by ChIP (chromatin immunoprecipitation) — quantitative PCR (qPCR) analysis. Results: RT-PCR analysis indicated that all 20 histone H2a genes assessed in this study are expressed. The replication-dependent histone H2a genes have different expression levels but similar expression patterns. Among the 20 histone H2a genes, the expression-level of H2afz, a replication-independent gene, was highest, and that of Hist1h2aa, a replication-dependent gene, was lowest. Among 18 replication-dependent H2a genes, the expression level of Hist3h2a was highest. The ChIP-qPCR analysis showed that histone H3 K9 acetylation levels in promoter regions of both H2afz and Hist3h2a are clearly higher than that in the promoter region of Hist1h2aa. The H3 K9 acetylation level in the promoter of Hist1h2aa is similar to that in the γ-satellite region. Conclusion: These results strongly suggest that histone H3 K9 acetylation plays a role in the expression of histone genes.
ACCESSION #
28858544

 

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