TITLE

Detrimental role for CD4+ lymphocytes in murine diffuse peritonitis due to inhibition of local bacterial elimination

AUTHOR(S)
Busse, M.; Traeger, T.; Pötschke, C.; Billing, A.; Dummer, A.; Friebe, E.; Kiank, C.; Grunwald, U.; Jack, R. S.; Schütt, C.; Heidecke, C-D .; Maier, S.; Bröker, B. M.
PUB. DATE
February 2008
SOURCE
Gut;Feb2008, Vol. 57 Issue 2, p188
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Abdominal sepsis due to intestinal leakage of endogenous gut bacteria is a life-threatening condition. In healthy individuals, T lymphocytes have essential functions in balancing the immune response to the commensal gut flora. Aim: To determine how T lymphocytes shape the process of diffuse faecal peritonitis. Methods: In colon ascendens stent peritonitis (CASP), a clinically relevant mouse model of diffuse peritonitis, the kinetics of systemic T cell activation were investigated by assessment of activation markers. CD4+ T cells were then depleted with monoclonal antibodies, and survival, bacterial dissemination and cytokine concentrations were measured. T cell receptor signalling was blocked with tacrolimus. Results: In diffuse peritonitis, CD4+ T cells, both Foxp3- and Foxp3+, became systemically involved within hours and upregulated CTLA-4 and other activation markers. Depletion of the CD4+ T cells enhanced local bacterial clearance from the peritoneal cavity, reduced bacterial dissemination and improved survival. This was accompanied by increased immigration of granulocytes and macrophages into the peritoneum, indicating that CD4+ T cells inhibit the local innate immune response. Blockade of T cell receptor (TCR) signalling by tacrolimus did not influence the survival in this peritonitis model, showing that the inhibitory effects of the CD4+ T lymphocytes were independent of TCR-mediated antigen recognition. Conclusion: In diffuse peritonitis caused by commensal gut bacteria the CD4+ T lymphocytes exert a net negative effect on the local anti-bacterial defence, and thereby contribute to bacterial dissemination and poor outcome.
ACCESSION #
28843110

 

Related Articles

  • Neonates with Culture Proven Sepsis Have Lower Amounts and Percentage of CD45RA+ T Cells. A. Aygun; A. Citak Kurt; Ahmet Godekmerdan; Abdullah Kurt; Saadet Akarsu; M. Gurgoze; Erdal Yilmaz // Inflammation;Aug2008, Vol. 31 Issue 4, p222 

    Abstract  To evaluate the expression of lymphocyte subsets in newborns diagnosed as culture proven or culture negative sepsis and to investigate the differentiation. The aim of this study is to explore neonatal immunology in newborns diagnosed as culture proven or culture negative neonatal...

  • A Peptide Binding to the β-Site of APP Improves Spatial Memory and Attenuates Aβ Burden in Alzheimer's Disease Transgenic Mice. Shi-gao Yang; Shao-wei Wang; Min Zhao; Ran Zhang; Wei-wei Zhou; Ya-nan Li; Ya-jing Su; He Zhang; Xiao-lin Yu; Rui-tian Liu // PLoS ONE;Nov2012, Vol. 7 Issue 11, Special section p1 

    Amyloid precursor protein cleaving enzyme 1 (BACE1), an aspartyl protease, initiates processing of the amyloid precursor protein (APP) into β-amyloid (Aβ); the peptide likely contributes to development of Alzheimer's disease (AD). BACE1 is an attractive therapeutic target for AD treatment,...

  • Interleukin-12 (IL-12p70) promotes induction of highly potent Th1-like CD4+CD25+T regulatory cells that inhibit allograft rejection in unmodified recipients. Verma, Nirupama D.; Hall, Bruce M.; Plain, Karren M.; Robinson, Catherine M.; Boyd, Rochelle; Tran, Giang T.; Chuanmin Wang; Bishop, G. Alex; Hodgkinson, Suzanne J. // Frontiers in Immunology;Apr2014, Vol. 5, p1 

    In rat models, CD4+CD25+ T regulatory cells (Treg) play a key role in the induction and maintenance of antigen-specific transplant tolerance, especially in DA rats with PVG cardiac allografts (1, 2). We have previously described generation of alloantigen-specific Treg (Ts1), by culture of...

  • Opinion-Regulatory Lymphocytes: Natural versus adaptive regulatory T cells. Bluestone, Jeffrey A.; Abbas, Abul K. // Nature Reviews Immunology;Mar2003, Vol. 3 Issue 3, p253 

    The regulation of immune responses to self-antigens is a complex process that involves maintaining self-tolerance while retaining the capacity to mount robust immune responses against invading microorganisms. Over the past few years, many new insights into this process have been gained, leading...

  • THYMOCYTE APOPTOSIS AND PROLIFERATION MODELING DURING RAT THYMIC INVOLUTION IS INFLUENCED BY OVARIAN HORMONES IN A THYMOCYTE SUBSET-SPECIFIC MANNER. NEVENA, ARSENOVIĆ-RANIN; MIRJANA, NACKA-ALEKSIĆ; JASMINA, DJIKIĆ; MILICA, PERIŠIĆ; D., KOSEC; I., PILIPOVIĆ; ZORICA, STOJIĆ-VUKANIĆ; GORDANA, LEPOSAVIĆ // Acta Veterinaria;2013, Vol. 63 Issue 1, p3 

    The study was aimed to define the putative role of ovarian hormones in shaping thymocyte apoptosis and proliferation during thymic involution. Thymocytes from young adult and middle-aged rats ovariectomized (Ox) before puberty were examined for apoptosis and proliferation. Apoptosis and...

  • Th17 Cell-Mediated Neuroinflammation Is Involved in Neurodegeneration of Aβ1-42-Induced Alzheimer’s Disease Model Rats. Zhang, Jun; Ke, Kai-Fu; Liu, Zhan; Qiu, Yi-Hua; Peng, Yu-Ping // PLoS ONE;Oct2013, Vol. 8 Issue 10, p1 

    Neuroinflammation, especially innate immunocyte-mediated neuroinflammation, has been reported to participate in pathogenesis of Alzheimer’s disease (AD). However, the involvement of adaptive immune cells, such as CD4+ T lymphocytes, in pathogenesis of AD is not well clarified. Herein, we...

  • CD4+ T-cell help controls CD8+ T-cell memory via TRAIL-mediated activation-induced cell death. Janssen, Edith M.; Droin, Nathalie M.; Lemmens, Edward E.; Pinkoski, Michael J.; Bensinger, Steven J.; Ehst, Benjamin D.; Griffith, Thomas S.; Green, Douglas R.; Schoenberger, Stephen P. // Nature;3/3/2005, Vol. 434 Issue 7029, p88 

    The‘help’provided by CD4+ T lymphocytes during the priming of CD8+ T lymphocytes confers a key feature of immune memory: the capacity for autonomous secondary expansion following re-encounter with antigen. Once primed in the presence of CD4+ T cells,‘helped’CD8+ T cells...

  • Deficiency of CD4[sup +] lymphocytes due to intestinal loss after Fontan procedure. Garty, Ben-Zion // European Journal of Pediatrics;2001, Vol. 160 Issue 1, p58 

    Reports on the clinical case of a patient with CD4[sup+] lymphocyte deficiency due to intestinal loss after a Fontan procedure. Description of the patient studied; Information on the laboratory data of the patient; Events of cellulitis in the legs of the patient; Presence of bacteria in the...

  • Immunological memory: How T cells survive. Bell, Jennifer // Nature Reviews Immunology;Jul2003, Vol. 3 Issue 7, p516 

    Presents the result of a study on the factors that regulate the size of the memory CD4 T-cell. Analysis of the role of T-cell receptor signals; Consequences created by the lack of transgene signals; Function of interleukin-7 in the T-cell.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics