TITLE

Molecular cloning and sequence analysis of hamster CENP-A cDNA

AUTHOR(S)
Figueroa, Javier; Pendón, Carlos; Valdivia, Manuel M.
PUB. DATE
January 2002
SOURCE
BMC Genomics;2002, Vol. 3, p11
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: The centromere is a specialized locus that mediates chromosome movement during mitosis and meiosis. This chromosomal domain comprises a uniquely packaged form of heterochromatin that acts as a nucleus for the assembly of the kinetochore a trilaminar proteinaceous structure on the surface of each chromatid at the primary constriction. Kinetochores mediate interactions with the spindle fibers of the mitotic apparatus. Centromere protein A (CENP-A) is a histone H3-like protein specifically located to the inner plate of kinetochore at active centromeres. CENP-A works as a component of specialized nucleosomes at centromeres bound to arrays of repeat satellite DNA. Results: We have cloned the hamster homologue of human and mouse CENP-A. The cDNA isolated was found to contain an open reading frame encoding a polypeptide consisting of 129 amino acid residues with a C-terminal histone fold domain highly homologous to those of CENPA and H3 sequences previously released. However, significant sequence divergence was found at the N-terminal region of hamster CENP-A that is five and eleven residues shorter than those of mouse and human respectively. Further, a human serine 7 residue, a target site for Aurora B kinase phosphorylation involved in the mechanism of cytokinesis, was not found in the hamster protein. A human autoepitope at the N-terminal region of CENP-A described in autoinmune diseases is not conserved in the hamster protein. Conclusions: We have cloned the hamster cDNA for the centromeric protein CENP-A. Significant differences on protein sequence were found at the N-terminal tail of hamster CENP-A in comparison with that of human and mouse. Our results show a high degree of evolutionary divergence of kinetochore CENP-A proteins in mammals. This is related to the high diverse nucleotide repeat sequences found at the centromere DNA among species and support a current centromere model for kinetochore function and structural plasticity.
ACCESSION #
28834623

 

Related Articles

  • cDNA Cloning and Expression Analysis of a Novel Human F-Box Only Protein. Haipeng Cheng; Yushu Ma; Xiaohua Ni; Min Jiang; Lingchen Guo; Wei Jin; Weiwen Xu; Gentao Cao; Chaoneng Ji; Kang Ying; Shaohua Gu; Yuhong Ma; Yi Xie; Yumin Mao // Molecules & Cells (Springer Science & Business Media B.V.);2002, Vol. 14 Issue 1, p56 

    Provides information on a study that cloned cDNA and analyzed expression of a novel human F-box only (FBXO) protein. Nature of F-box proteins; Identification of the human FBXO16 cDNA; Gene structure and chromosomal localization of the human FBXO gene.

  • Molecular Cloning, Occurrence, and Expression of a Novel Partially Secreted Protein "Psoriasin" That Is Highly Up-Regulated in Psoriatic Skin. Madsen, Peder; Rasmussen, Hanne H.; Leffers, Henrik; Honoré, Bent; Dejgaard, Kurt; Olsen, Eydfinnur; Kiil, Jette; Walbum, Else; Andersen, Annette H.; Basse, Bodil; Lauridsen, Jette B.; Ratz, Gitte P.; Celis, Ariana; Vandekerckhove, Joel; Celis, Julio E. // Journal of Investigative Dermatology;Oct91, Vol. 97 Issue 4, p701 

    Analysis of the protein pattern of normal and psoriatic noncultured unfractionated keratinocytes has revealed several low-molecular-weight proteins that are highly up-regulated in psoriatic epidermis. Here, we have cloned and sequenced the cDNA (clone 1085) for one of these proteins that we have...

  • Molecular Cloning, Genomic Organization, Promoter Activity, and Tissue-Specific Expression of the Mouse Ryudocan Gene1. Tsuzuki, Shinobu; Kojima, Tetsuhito; Katsumi, Akira; Yamazaki, Tomio; Sugiura, Isamu; Saito, Hidehiko // Journal of Biochemistry;1997, Vol. 122 Issue 1, p17 

    Ryudocan, a ubiquitous heparan sulfate proteoglycan, is a member of the syndecan family of cell surface proteoglycans. The full-length cDNA encoding the murine ryudocan core protein has now been cloned and sequenced. The deduced primary structure of mouse ryudocan, including the three...

  • kinetochore.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p1271 

    A reference entry for "kinetochore" is presented, which refers to a protein disk attached to the DNA of the centromere.

  • Molecular cloning of a complementary DNA to rat cyclophilin-like protein mRNA. Iwai, Naoharu; Inagami, Tadashi // Kidney International;Jun1990, Vol. 37 Issue 6, p1460 

    Using the technique of differential plaque filter hybridization, a rat cDNA was isolated whose corresponding gene expression in the kidney was positively modulated up to threefold by sodium depletion. This mRNA was more abundantly expressed in the kidneys of 17-week-old spontaneously...

  • New families of site-specific repetitive DNA sequences that comprise constitutive heterochromatin of the Syrian hamster ( Mesocricetus auratus, Cricetinae, Rodentia). Yamada, Kazuhiko; Kamimura, Eikichi; Kondo, Mariko; Tsuchiya, Kimiyuki; Nishida-Umehara, Chizuko; Matsuda, Yoichi // Chromosoma;Feb2006, Vol. 115 Issue 1, p36 

    We molecularly cloned new families of site-specific repetitive DNA sequences from BglII- and EcoRI-digested genomic DNA of the Syrian hamster ( Mesocricetus auratus, Cricetrinae, Rodentia) and characterized them by chromosome in situ hybridization and filter hybridization. They were classified...

  • Centromere Replication Timing Determines Different Forms of Genomic Instability in Saccharomyces cerevisiae Checkpoint Mutants During Replication Stress. Wenyi Feng; Bachant, Jeff; Collingwood, David; Raghuraman, M. K.; Brewer, Bonita J. // Genetics;Dec2009, Vol. 183 Issue 4, p1249 

    Yeast replication checkpoint mutants lose viability following transient exposure to hydroxyurea, a replication-impeding drug. In an effort to understand the basis for this lethality, we discovered that different events are responsible for inviability in checkpoint-deficient cells harboring...

  • Molecular biology and regulation of nucleoside and nucleobase transporter proteins in eukaryotes and prokaryotes. Cabrita, Miguel A; Baldwin, Stephen A; Young, James D; Cass, Carol E // Biochemistry & Cell Biology;Oct2002, Vol. 80 Issue 5, p623 

    The molecular cloning of cDNAs encoding nucleoside transporter proteins has greatly advanced understanding of how nucleoside permeants are translocated across cell membranes. The nucleoside transporter proteins identified thus far have been categorized into five distinct superfamilies. Two of...

  • Molecular cloning and characterization of a cDNA encoding the N-acetyl-β-D-glucosaminidase homologue of Paracoccidioides brasiliensis. Santos, Mônica O.; Pereira, Maristela; Felipe, Maria Sueli S.; Jesuino, Rosalia Santos A.; Ulhoa, Cirano J.; Soares, Renata De Bastos A.; Soares, Celia Maria De A. // Medical Mycology;Jun2004, Vol. 42 Issue 3, p247 

    A cDNA encoding the N-acetyl-β-D-glucosaminidase (NAG) protein of Paracoccidioides brasiliensis , Pb NAG1, was cloned and characterized. The 2663-nucleotide sequence of the cDNA consisted of a single open reading frame encoding a protein with a predicted molecular mass of 64.73 kDa and an...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics