Lubricin Surface Modification Improves Extrasynovial Tendon Gliding in a Canine Model in Vitro

Taguchi, Manabu; Yu-Long Sun; Chunfeng Zhao; Zobitz, Mark E.; Chung-Ja Cha; Jay, Gregory D.; Kai-Nan An; Amadio, Peter C.
January 2008
Journal of Bone & Joint Surgery, American Volume;Jan2008, Vol. 90-A Issue 1, p129
Academic Journal
Background: Lubricin is the principal lubricant in synovial fluid. Although lubricin has been identified in tendons, especially on the surface of intrasynovial tendons such as the flexor digitorum profundus tendon, its ability to improve tendon gliding is unknown. The purpose of this study was to investigate the effects of exogenously applied lubricin on the gliding of extrasynovial tendons in a canine model in vitro. Methods: Forty peroneus longus tendons, along with the proximal pulley in the ipsilateral hind paw, were harvested from adult mongrel dogs. After the gliding resistance of the normal tendons was measured, the tendons were treated with one of the following solutions: saline solution, lubricin, carbodiimide derivatized gelatin (cd-gelatin), carbodiimide derivatized gelatin with hyaluronic acid (cd-HA-gelatin), or carbodiimide derivatized gelatin to which lubricin had been added in a second step (cd-gelatin plus lubricin). Tendon gliding resistance was measured for 1000 cycles of simulated flexion-extension motion of the tendon. Transverse sections of the tendons were examined qualitatively at 100x magnification to estimate surface smoothness after 1000 cycles. Results: There was no significant difference in the gliding resistance between the tendons treated with saline solution and those treated with lubricin alone, or between the tendons treated with cd-HA-gelatin and those treated with cd-gelatin plus lubricin; however, the gliding resistance of the tendons treated with cd-gelatin plus lubricin was significantly lower than that of the tendons treated with saline solution, lubricin alone, or cd-gelatin alone (p < 0.05). After 1000 cycles of tendon motion, the gliding resistance of the tendons treated with cd-gelatin plus lubricin decreased 18.7% compared with the resistance before treatment, whereas the gliding resistance of the saline-solution-treated controls increased >400%. The tendon surfaces treated with cd-gelatin plus lubricin or with cd-HA-gelatin appeared smooth even after 1000 cycles of tendon motion, whereas the other surfaces appeared roughened. Conclusions: While the addition of lubricin alone did not affect friction in this tendon gliding model, the results indicate that lubricin may preferentially adhere to a tendon surface pretreated with cd-gelatin and, when so fixed in place, lubricin does have an important effect on tendon lubrication. Clinical Relevance: This in vitro canine study suggests that modification of a tendon surface with lubricin can improve the tendon's gliding ability, potentially improving clinical outcomes of extrasynovial tendon grafting.


Related Articles

  • Dickkopf-1 (Dkk-1) in plasma and synovial fluid is inversely correlated with radiographic severity of knee osteoarthritis patients. Honsawek, Sittisak; Tanavalee, Aree; Yuktanandana, Pongsak; Ngarmukos, Srihatach; Saetan, Natthaphon; Tantavisut, Saran // BMC Musculoskeletal Disorders;2010, Vol. 11, p257 

    Background: Osteoarthritis (OA) is a common degenerative joint disease causing pain, stiffness, reduced motion, swelling, crepitus, and disability. Dickkopf-1 (Dkk-1) is a critical mediator of osteoblastogenesis and regulates the joint remodeling. The aim of this study was to examine plasma and...


    The development of the therapeutic paradigm viscosupplementation for long-lasting pain relief in human and animal joints with osteoarthritis or traumatic arthritis was based on the finding that in arthritic conditions, the average molecular weight and concentration of hyaluronan decreased and...

  • Biomechanical Properties and Collagen Fiber Orientation of TMJ Discs in Dogs: Part 1. Gross Anatomy and Collagen Fiber Orientation of the Discs. Teng Shengyi; Xu Yinghua // Journal of Craniomandibular Disorders;Winter1991, Vol. 5 Issue 1, p28 

    The present study investigated the gross anatomy and collagen fiber orientation of TMJ discs in dogs using macroscopic, stereomicroscopic, light microscopic, and scanning electron microscopic techniques. Results showed that (1) the surface of the disc has an undulated configuration (a...

  • The Early History of Synovia (Joint Fluid). Rodnan, Gerald P.; Beneder, Thomas G.; Panetta, William C. // Annals of Internal Medicine;Oct66, Vol. 65 Issue 4, p821 

    Deals with the history of the synovia or joint fluid. Greek and Roman descriptions; Origin of the term synovia; List of the synonyms applied to synovia or joint fluid.

  • Involvement of TWEAK/Fn14 interaction in the synovial inflammation of RA. S. Kamijo; A. Nakajima; K. Kamata; H. Kurosawa; H. Yagita; K. Okumura // Rheumatology;Apr2008, Vol. 47 Issue 4, p442 

    Objective. TWEAK, TNF-like weak inducer of apoptosis, induces not only apoptosis of some tumour cells, but also proliferation of endothelial cells, and angiogenesis. It is known that TWEAK induces production of cytokines that are involved in the pathogenesis of RA. However, it is not clear how...

  • WstÄ™pne obliczenia wpÅ‚ywu mikrostruktury mazi stawowej na trybologiÄ™ bioÅ‚ożyska. Kucaba-Piętal, Anna // Systems: Journal of Transdisciplinary Systems Science;2006, Vol. 11 Issue 1, p197 

    The micropolar fluid theory was applied to answer the question how the microstructure dependence of synovial fluid influences on tribological quantities of biobearing. The squeezing flow of micropolar fluid between two parallel circular plates was considered, as an approximation for the...

  • Viscoelastic Effects on the Unsteady Convective Diffusion in a Synovial Fluid of Human Joints. Bali, Rekha; Sharma, S. K. // International Journal of Theoretical & Applied Mechanics;2009, Vol. 4 Issue 2, p119 

    A generalized dispersion model is used to obtain exact solution for unsteady convective diffusion in a synovial fluid of human joints. In this paper, synovial fluid is represented by viscoelastic fluid. Analytically the problem is formulated as a two region namely diffusion and flow model. Flow...

  • Age-related changes and sex differences in chondroitin sulfate isomers and hyaluronic acid in normal synovial fluid. Uesaka, Shinji; Miyazaki, Kyosuke; Ito, Hiromoto // Modern Rheumatology (Springer Science & Business Media B.V.);Dec2004, Vol. 14 Issue 6, p470 

    The effects of factors such as age and sex on the metabolism of chondroitin sulfate (CS) and hyaluronic acid (HA) in knee joint tissues are believed to be profoundly important in the onset of joint diseases including osteoarthritis. To test whether age and sex influence CS isomers and HA in...

  • Synovial fluid levels of E-selectin and intercellular adhesion molecule-1: relationship to joint inflammation in children with chronic arthritis. Bloom, Bradley J.; Nelson, Sarah M.; Alario, Anthony J.; Miller, Laurie C.; Schaller, Jane G. // Rheumatology International;Sep2002, Vol. 22 Issue 5, p175 

    E-selectin and intercellular adhesion molecule (ICAM)-1 are crucial to the inflammatory response in chronic inflammatory arthritis. Soluble (s) levels of these molecules in sera and synovial fluid (SF) correlate with some clinical parameters and synovial tissue expression of the same molecules...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics