TITLE

Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography

AUTHOR(S)
Müller, Jens; El-Maarri, Osman; Oldenburg, Johannes; Pötzsch, Bernd; Mayer, Günter
PUB. DATE
February 2008
SOURCE
Analytical & Bioanalytical Chemistry;Feb2008, Vol. 390 Issue 4, p1033
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Monitoring of in-vitro selection experiments is crucial in evaluation of the success and outcome of such approaches. Furthermore, monitoring running in parallel with the selection procedure enables early intervention and adjustment of stringency to achieve the desired activities of the selected nucleic acid species. Here we describe the use of a non-radioactive method that enables monitoring of a SELEX procedure on the basis of sequence diversity. We employ denaturing HPLC and describe for the first time an experimental set-up that is useful both for analysis of the progression of in-vitro selection experiments and for separation of distinct aptamer sequences.
ACCESSION #
28804040

 

Related Articles

  • Denaturing HPLC of Nucleic Acids. Premstaller, Andreas; Oefner, Peter J. // LC-GC Europe;Jul2002, Vol. 15 Issue 7, p410 

    Discusses the denaturing high performance liquid chromatography (HPLC) of nucleic acids. Principle of partially denaturing HPLC; Sensitivity and specificity of denaturing HPLC; Denaturing HPLC in comparison with other methods commonly used in mutation analysis; Primer extension reactions; Mass...

  • Selenium and vitamin E concentrations in human milk and formula milk from Hungary. Sziklai-László, I.; Majchrzak, D.; Elmadfa, I.; Cser, M. Á. // Journal of Radioanalytical & Nuclear Chemistry;Feb2009, Vol. 279 Issue 2, p585 

    The metabolic roles of vitamin E and selenium are closely related, and to a very great extent, each can compensate for the deficiency of the other. The aim of the study was to determine and compare the Se and vitamin E (α- and γ-tocopherol) contents of breast milk and commercially...

  • Analysis of bioactive ingredients in the brown algaFucus vesiculosusby capillary electrophoresis and neutron activation analysis. Truus, Katie; Vaher, Merike; Koel, Mihkei; Mähar, Andres; Taure, Imants // Analytical & Bioanalytical Chemistry;Jul2004, Vol. 379 Issue 5/6, p849 

    Two different types of bioactive components of the seaweedFucus vesiculosuswere analysed: (1) polyphenols (phlorotannins) by capillary electrophoresis (CE) and (2) mineral part (including bioactive microelements) by neutron activation analysis (NAA). CE experiments were carried out using a UV...

  • ANTIOXIDATIVE PEPTIDES DERIVED FROM DENATURATED EGG WHITE PROTEIN. ZAMBROWICZ, A.; POKORA, M.; ECKERT, E.; CHRZANOWSKA, J.; SZOŁTYSIK, M.; DĄBROWSKA, A.; TRZISZKA, T. // Italian Journal of Food Science;2013, Vol. 25 Issue 2, p169 

    The antioxidant activity of denaturated egg white protein preparation (EP) was evaluated by enzymatic hydrolysis. The EP treated with digestive and microbial proteases resulted in different degradation products confirmed by degree of hydrolysis (DH) and free amino groups concentration (FAG)...

  • A novel extraction method of plasma peptides for peptidomics analysis. Yu Liu; Xiaohong Xun; Hui Li; Li Liu; Dongsong Nie; Yang Xiang; Jianming Yi; Jizu Yi // Journal of Chemical & Pharmaceutical Research;2014, Vol. 6 Issue 10, p291 

    Different methods for plasma peptidome preparation in peptidomics analysis had been compared in this study. Plasma proteins were either precipitated with acetonitrile or subjected to themal denaturation. The eluent was subjected to analyze by gel filtration high pressure liquid chromatography....

  • Technological developments in lipidomics. Hou, Weimin; Zhou, Hu; Elisma, Fred; Bennett, Steffany A. L.; Figeys, Daniel // Briefings in Functional Genomics & Proteomics;Sep2008, Vol. 7 Issue 5, p395 

    Lipid analysis is a well-established field of research that focuses on one lipid or a few lipids. The recent developments in mass spectrometry technologies have enabled more comprehensive studies to be performed on lipids present in a sample. The move towards extensive lipid research has led to...

  • Development of the HPLC method to determine the metabolites of isomajdine isolated from Vinca herbacea Waldst. et Kit. growing in Georgia. Gagua, Natia; Aubert, C.; Vachnadze, Valentina; Bakuridze, Aliosha // Journal of Natural Pharmaceuticals;Jul2011, Vol. 2 Issue 3, p163 

    Background: It was decided to develop a reliable HPLC method coupled with ultraviolet detection for simultaneous determination of isomajdine and its metabolites in in vitro experiment. Methods: A liquid liquid extraction was used to extract the compounds from plasma. The analysis was carried out...

  • The UHPLC-DAD fingerprinting method for analysis of extracellular metabolites of fungi of the genus Geosmithia (Acomycota: Hypocreales). Tylová, Tereza; Kolarík, Miroslav; Olšovská, Jana // Analytical & Bioanalytical Chemistry;Oct2011, Vol. 400 Issue 9, p2943 

    new simple ultra-high-performance liquid chromatography method with diode array detection (UHPLC-DAD) was developed for chemical fingerprinting analysis of extracellular metabolites in fermentation broth of Geosmithia spp. The SPE method employing Oasis MCX strong cation-exchange mixed-mode...

  • Identification and quantification of gossypol in cotton by using packed micro-tips columns in combination with HPLC. Meyer, R.; Vorster, S.; Dubery, I. // Analytical & Bioanalytical Chemistry;Oct2004, Vol. 380 Issue 4, p719 

    Self-packed micro-tip columns containing a C18-bonded silica stationary phase, based on the same principles as solid-phase extraction methods, were used to obtain gossypol and related sesquiterpenoid aldehyde-enriched fractions. The enriched metabolite fractions were then analyzed by optimized...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics