TITLE

Isolation and purification assay of ex vivo photosystem II D1 protein toward integrated biointeraction analysis

AUTHOR(S)
Muktiono, B.; Schulten, C.; Heemken, O.; Gandrass, J.; Prange, A.; Schnabl, H.; Cerboncini, C.
PUB. DATE
February 2008
SOURCE
Analytical & Bioanalytical Chemistry;Feb2008, Vol. 390 Issue 4, p1195
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Protein extracts of photosystem II were prepared from leaf chloroplasts of different plant species by fast and nondenaturing methods. Sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blot analysis of the proteins obtained showed that the extracts were enriched by D1 proteins, which appeared putatively in association with the 33-kDa oxygen-evolving-complex subunits. In further isolation steps D1 proteins were purified using salt-gradient chromatography (fast protein liquid chromatography) and characterized by western blot and mass spectrometry.
ACCESSION #
28804036

 

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