TITLE

T-cell and serological responses to Erp, an exported Mycobacterium tuberculosis protein, in tuberculosis patients and healthy individuals

AUTHOR(S)
Martinez, Valérie; Carcelain, Guislaine; Badell, Edgar; Jouan, Marc; Mauger, Isabelle; Sellier, Pierre; Truffot, Chantal; Bricaire, François; Arend, Sandra M.; Ottenhoff, Tom; Autran, Brigitte; Gicquel, Brigitte
PUB. DATE
January 2007
SOURCE
BMC Infectious Diseases;2007, Vol. 7 Issue 1, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: The identification of antigens able to differentiate tuberculosis (TB) disease from TB infection would be valuable. Cellular and humoral immune responses to Erp (Exported repetitive protein) - a recently identified M. tuberculosis protein - have not yet been investigated in humans and may contribute to this aim. Methods: We analyzed the cellular and humoral immune responses to Erp, ESAT-6, Ag85B and PPD in TB patients, in BCG+ individuals without infection, BCG+ individuals with latent TB infection (LTBI) and BCG- controls. We used lymphoproliferation, ELISpot IFN-?, cytokine production assays and detection of specific human antibodies against recombinant M. tuberculosis proteins. Results: We included 22 TB patients, 9 BCG+ individuals without TB infection, 7 LTBI and 7 BCGcontrols. Erp-specific T cell counts were higher in LTBI than in the other groups. Erp-specific T cell counts were higher in LTBI subjects than TB patients (median positive frequency of 211 SFC/106 PBMC (range 118-2000) for LTBI subjects compared to 80 SFC/106 PBMC (range 50-191), p =0.019); responses to PPD and ESAT-6 antigens did not differ between these groups. IFN-? secretion after Erp stimulation differed between TB patients and LTBI subjects (p = 0.02). Moreover, LTBI subjects but not TB patients or healthy subjects produced IgG3 against Erp. Conclusion: The frequencies of IFN-γ-producing specific T cells, the IFN-γ secretion and the production of IgG3 after Erp stimulation are higher in LTBI subjects than in TB patients, whereas PPD and ESAT-6 are not.
ACCESSION #
28796498

 

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