Metabolic acidosis inhibits soft tissue calcification in uremic rats

Mendoza, F. J.; Lopez, I.; Montes de Oca, A.; Perez, J.; Rodriguez, M.; Aguilera-Tejero, E.
February 2008
Kidney International;Feb2008, Vol. 73 Issue 4, p407
Academic Journal
Metabolic acidosis is common in patients with chronic kidney disease, which is known to affect bone metabolism. We examined the effect of metabolic acidosis on the development of vascular and other soft-tissue calcifications in uremic rats treated with calcitriol. Extraskeletal calcification was measured in vivo, in control rats and rats with a remnant kidney model of uremia with or without ammonium chloride-induced acidosis. Soft-tissue calcification was assessed histologically, by measurement of the expression of the sodium-dependent phosphate cotransporter Pit-1 and by quantification of tissue calcium and phosphorus. Calcitriol administration to uremic rats resulted in significant deposition of material positive for von Kossa stain in the aorta, stomach, and kidney, elevated aortic calcium and phosphorus, increased aortic Pit-1 expression, and high mortality. Calcitriol-treated uremic rats with acidosis did not develop aortic or soft-tissue calcification, did not increase aortic Pit-1 expression, and had significantly lower mortality. Additionally, an acidotic environment prevented calcification of vascular smooth muscle cells in vitro. Our study shows that metabolic acidosis inhibits extraskeletal calcification.Kidney International (2008) 73, 407–414; doi:10.1038/sj.ki.5002646; published online 7 November 2007


Related Articles

  • Metabolic acidosis and vascular calcification: Using blueprints from bone to map a new venue for vascular research. Al-Aly, Z. // Kidney International;Feb2008, Vol. 73 Issue 4, p377 

    Vascular calcification is an important problem in patients with chronic kidney disease. The pathobiology of vascular calcification is complex and is intricately related to bone remodeling. Mendoza et al. report that experimental metabolic acidosis prevents calcitriol-induced vascular...

  • ketoacidosis. Peters, Michael // BMA A-Z Family Medical Encyclopedia;2004, p446 

    An encyclopedia entry for "ketoacidosis," a combination of acidosis and ketosis, is presented.

  • Rapid diagnosis of alcoholic ketoacidosis by proton NMR. Godet, Cendrine; Hira, Michel; Adoun, Michèle; Eugène, Michel; Robert, René // Intensive Care Medicine;Apr2001, Vol. 27 Issue 4, p785 

    In alcoholic patients, metabolic acidosis can be related to lactate acidosis associated with sepsis or thiamine deficiency, ketoacidosis, methanol or ethylene glycol poisoning. High resolution proton nuclear magnetic resonance (NMR) can be used to detect abnormal organic acid metabolites in...

  • Cerebrovascular Vasodilation to Extraluminal Acidosis Occurs via Combined Activation of ATP-Sensitive and Ca2+-Activated Potassium Channels. Ute Lindauer // Journal of Cerebral Blood Flow & Metabolism;Oct2003, Vol. 23 Issue 10, p1227 

    SUMMARY: Albeit controversely discussed, it has been suggested by several authors that nitric oxide (NO) serves as a permissive factor in the cerebral blood flow response to systemic hypercapnia. Potassium channels are important regulators of cerebrovascular tone and may be modulated by a basal...

  • Seasonal variations of pH, pCO2, pO2, HCO3− and Ca2+ in the haemolymph: implications on the calcification physiology in Anodonta cygnea. Lopes-Lima, Manuel; Lopes, Anabela; Casaca, Paula; Nogueira, Isabel; Checa, António; Machado, Jorge // Journal of Comparative Physiology B: Biochemical, Systemic & Env;Apr2009, Vol. 179 Issue 3, p279 

    A study about the relationship between the physical–chemical parameters and the calcium carbonate balance between the haemolymph fluid and mantle calcareous structures was carried out in Anodonta cygnea. An intense peak of HCO3− and a highest pH in December–January months may...

  • Optimal correction of acidosis changes progression of dialysis osteodystrophy. Lefebvre, Alfred; De Vernejoul, Marie Christine; Gueris, Jean; Goldfarb, Bernard; Graulet, Anne Marie; Morieux, Caroline // Kidney International;Dec1989, Vol. 36 Issue 6, p1112 

    To investigate an eventual role of acidosis on hemodialysis osteodystrophy we prospectively studied 21 patients who were dialyzed with different amounts of bicarbonate in the dialysate for 18 months. According to the level of bone formation rate (BFR) on a prestudy bone biopsy, patients were...

  • Serious cardiac and pulmonary calcification in a young peritoneal dialysis patient: potential role of continuous correction of acidosis. Nakagawa, M.; Kise, K.; Okamoto, N.; Fujino, H.; Iwai, M.; Nomura, Y.; Sawa, H. // Clinical Nephrology;2005, Vol. 63 Issue 4, p313 

    We describe a 40-month-old male infant with renal failure, treated with peritoneal dialysis, who developed massive calcification of soft tissues including the heart and lungs with subsequent cardiopulmonary insufficiency. A diagnosis of Jeune syndrome was made. After starting peritoneal...

  • Lanthanum carbonate prevents accelerated medial calcification in uremic rats: role of osteoclast-like activity. Yu Che; Chen Bing; Javed Akhtar; Zhao Tingting; Yu Kezhou; Wang Rong // Journal of Translational Medicine;2013, Vol. 11 Issue 1, p2 

    Background Arterial medial calcification (AMC) is frequent prevalence in patients with end stage renal disease. Evidence about hyperphosphatemia induced anabolic crosstalk between osteoblast and osteoclast in AMC of uremia is rare. Lanthanum carbonate as an orally administered phosphate-binding...

  • Acid-base imbalance and the skeleton. Bushinsky, David A. // European Journal of Nutrition;2001, Vol. 40 Issue 5, p238 

    Summary Humans generally consume a diet that generates metabolic acids leading to a reduction in the concentration of systemic bicarbonate and a fall in pH. In vitro experiments indicate that this metabolic acidosis causes a release of calcium from bone that initially is simply due to...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics