Decreased expression of β1- and β2-adrenoceptors in human diabetic atrial appendage

Dinçer, U. Deniz; Güner, Şahika; Tay, Aydin; Ario&;#x011F;lu, Ebru; Taşdelen, Atilay; Aşlamaci, Sait; Bidasee, Keshore R.
January 2003
Cardiovascular Diabetology;2003, Vol. 2, p1
Academic Journal
Background: Using the streptozotocin-induced diabetic rat model, we have recently showed that the expression and function of β1-adrenoreceptor were decreased in the diabetic rat heart. However, the effect of diabetes on expression of β-adrenoreceptors in human cardiac tissue remains undefined. Therefore, the focus of the present study was to investigate the effect of diabetes on mRNA encoding β1- and β2-ARs in human atrial tissues. Methods: Right atrial appendages from five diabetic (mean age 65 ± 4.5; 4 female, 1 male) and five nondiabetic patients (mean age 56.2 ± 2.8; 4 male, 1 female) undergoing coronary artery bypass grafting were collected and assayed using reverse transcriptase-polymerase chain reaction (RTPCR) for their mRNA content. No patient from these two groups suffered from acute myocardial infarction and/or failure. All diabetic patients received insulin for at least two years and had been diagnosed as diabetics for at least five years. Results: When compared with levels in nondiabetics, steady state levels of mRNA encoding β1-adrenoreceptor decreased by 69.2 ± 7.6 % in diabetic patients while #x03B2;2-adrenoreceptor mRNA decreased by 32.2 ± 5.5 % (p < 0.001). Conclusions: Our findings show a decreased expression of β1- and β2-adrenoreceptors in human diabetic atrial appendage.


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